Heterorhabditis alii n. sp. (Nematoda: Heterorhabditidae), a novel entomopathogenic nematode from Egypt used against the fall armyworm, Spodoptera frugiperda (Smith 1797) (Lepidoptera: Noctuidae)

Egyptian Journal of Biological Pest Control

Table 3 Mortality rate of Spodoptera frugiperda for four larval instars induced by three concentrations of infective juveniles (IJs) for two native and one foreign heterorhabditid species in three time intervals

Nematode species	Time in hours
	24			48			72
	IJs/ml./3 larvae*			IJs/ml./3 larvae*			IJs/ml./3 larvae*
	100	250	500	100	250	500	100	250	500
	S. frugiperda 3rd instar larvae
Heterorhabditis. alii	67^bcd	78^bcd	100^a	89^ab	100^a	100^a	100^a	100^a	100^a
Heterorhabditis. bacteriophora (HP88)	22^fgh	33^efg	44^def	67^bcd	67^bcd	89^ab	100^a	100^a	100^a
H. indica (Mango 2)	11^gh	22^fgh	22^fgh	56^cde	56^cde	67^bcd	100^a	100^a	100^a
LSD value at 0.05	22.909
LSD value at 0.05	4th instar larvae
H. alii	56^cde	78^abc	100^a	89^ab	100^a	100^a	100^a	100^a	100^a
H. bacteriophora (HP88)	22^fgh	33^efg	22^fgh	67^bcd	56^cde	67^bcd	89^ab	100^a	100^a
H. indica (Mango 2)	11^gh	22^fgh	11^gh	56^cde	44^def	78^abc	89^ab	100^a	100^a
LSD value at 0.05	22.874
5th instar larvae
H. alii	44^def	67^bcd	100^a	89^ab	100^a	100^a	89^ab	100^a	100^a
H. bacteriophora (HP88)	11^gh	11^gh	22^fgh	44^def	44^def	56^cde	78^abc	100^a	100^a
H. indica (Mango 2)	0^h	11^gh	11^gh	33^efg	44^def	44^def	89^ab	89^ab	100^a
LSD value at 0.05	22.807
6th instar larvae
H. alii	33^f	44^ef	78^bc	78^bc	100^a	100^a	89^ab	100^a	100^a
H. bacteriophora (HP88)	0^g	11^g	11^g	44^ef	44^ef	56^de	78^bc	100^a	100^a
H. indica (Mango 2)	0^g	0^g	11^g	33^f	44^ef	44^ef	67^cd	89^ab	100^a
LSD value at 0.05	21.333