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Table 5 qPCR analysis: Relative fold change expression of Cad (cadherin), ALP (alkaline phosphatase), APN (aminopeptidase N), Try (trypsin), and CYP (cytochrome P450) in midgut of susceptible (Sus.), and Bt Cry1C tolerant (Tol.) strain of S. littoralis

From: Alterations in the expression of certain midgut genes of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and midgut histopathology in response to Bacillus thuringiensis Cry1C toxin

  Cad ALP APN Try CYP
ΔCt of Sus. ΔCt of Tol. ΔCt of Sus. ΔCt of Tol. ΔCt of Sus. ΔCt of Tol. ΔCt of Sus. ΔCt of Tol. ΔCt of Sus. ΔCt of Tol.
Replica 1 12.57 14.45 5.64 6.25 6.46 6.15 1.24 1.85 10.76 10.25
Replica 2 12.5 14.29 5.6 5.99 6.15 6.29 0.85 2.26 10.94 10.29
Replica 3 12.4 14.23 5.67 6.23 6.4 6.03 1.19 2.13 10.64 10.13
Average ΔCt 12.49 14.33 5.63 6.15 6.33 6.15 1.09 2.08 10.78 10.22
Fold of change = 2ΔΔCt 1.00 0.28 1.00 0.69 1.00 1.13 1.00 0.51 1.00 1.47
% of gene transcripts 72% downregulation 31% downregulation 13% not differ significantly 49% downregulation 47% significantly over-transcribed