Table 5 qPCR analysis: Relative fold change expression of Cad (cadherin), ALP (alkaline phosphatase), APN (aminopeptidase N), Try (trypsin), and CYP (cytochrome P450) in midgut of susceptible (Sus.), and Bt Cry1C tolerant (Tol.) strain of S. littoralis

Replica 1

12.57

14.45

5.64

6.25

6.46

6.15

1.24

1.85

10.76

10.25

Replica 2

12.5

14.29

5.6

5.99

6.15

6.29

0.85

2.26

10.94

10.29

Replica 3

12.4

14.23

5.67

6.23

6.4

6.03

1.19

2.13

10.64

10.13

Average ΔCt

12.49

14.33

5.63

6.15

6.33

6.15

1.09

2.08

10.78

10.22

Fold of change = 2^ΔΔCt

1.00

0.28

1.00

0.69

1.00

1.13

1.00

0.51

1.00

1.47

% of gene transcripts