Egg parasitoids belonging to the genus Trichogramma (Hymenoptera: Trichogrammatidae) are important natural enemies that have been successfully used in biological control programs and mostly reared on the Mediterranean flour moth, Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae). Host quality is a crucial factor that can determine parasitoid fitness.
Laboratory studies were carried out to evaluate the effects of 3 different larval diets CY diet (95% cornmeal + 5% yeast), CBGY diet (53.3% cornmeal + 26.7% wheat bran + 15% glycerine + 5% yeast), and WBGY diet (53.3% wheat flour + 26.7% wheat bran + 15% glycerine + 5% yeast) on some demographic parameters of E. kuehniella. Moreover, the performance of the species Trichogramma evanescens Westwood reared on the E. kuehniella eggs reared on the 3 artificial diets were determined. For the E. kuehniella rearing, the CY diet had a higher fecundity and greater emergence ratios. Nevertheless, CY diet also provided shorter developmental time, greater fecundity, and relatively higher female ratios of subsequent generations of the parasitoids.
The results suggest that the CY diet could be considered as a standard diet for the multigenerational rearing of T. evanescens.
Hymenopteran parasitoids are a key group of beneficial organisms that are used in augmentative biological control programs because they are more specific than predators and have a much more restricted host range, which is considered important in preventing undesirable side effects (Bigler et al. 2006; van Lenteren 2012). Egg parasitoids of the genus Trichogramma are important biological control agents that have been successfully used against a wide range of lepidopteran pests for more than 100 years via conservation strategies, artificial releases alone (El-Arnaouty et al. 2014; Wang et al. 2014) or in combination with insecticide applications (Parsaeyan et al. 2020). Ephestia kuehniella Zeller, Corcyra cephalonica (Strainton), Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae), Sitotroga cerealella (Olivier) (Lepidoptera: Gelechiidae), and Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae) are the most commonly used factitious hosts worldwide for the rearing of Trichogramma parasitoids (Abd El-Hafez 2001; El-Wakeil 2007; Wang et al. 2014).
Mass production of natural enemies is the base of augmentative biological control that is often a commercial activity because of the need for large scale regular releases of beneficial insects (van Lenteren 2012). Mass-produced natural enemies must compete with other pest control technologies to become commercially viable and sustainable. Producers of commercial natural enemies throughout the world have developed a variety of simple and highly productive rearing systems for Trichogramma spp. (Morales-Ramos et al. 2014). However, the costs of the host production are one of the most important parameters that can directly impact mass-rearing investments. Generally, factitious hosts are used to reduce the cost or to increase the efficiency of the mass-reared parasitoids (El-Wakeil 2007). E. kuehniella can be reared on basic and inexpensive diets, such as wheat/corn flour and many other cereal products (Locatelli et al. 2008). There have been several attempts to reduce the host costs with basic or complex diets (Farahani et al. 2016; Moghaddassi et al. 2019).
However, the economic optimization of the host mass rearing is not the unique factor that should be considered when defining a parasitoid commercial rearing. Indeed, the performance of parasitoids reared on cheaper hosts should be also taken into account for successful and sustainable mass-rearing and pest control performances (Ali and Wright 2020). For the egg parasitoids that all their immature stages develop inside the host, the host represents the sole source of nutrients (Farahani et al. 2016). The characteristics of host egg quality such as nutritional content, egg volume, chorion thickness, and size directly affect the parasitoid wasp fitness (Abd El-Hafez 2001; El-Wakeil 2007). Determining the role of host diet on the development and other biological parameters of Trichogramma spp. could improve the success of biological control programs.
Consequently, the aim of this study was to determine firstly: (i) the effects of different diets on some biological parameters of the factitious host, E. kuehniella, and (ii) biological traits of T. evanescens on the eggs of E. kuehniella fed on three different diets.
Materials and methods
Rearing of insects
The initial colony of E. kuehniella was derived from the Biological Control Research Institute, Adana, Turkey. The moths have been maintained in the laboratory for 3 years before being used in the experiments. Its larvae were reared on a wheat flour: wheat bran (2:1) mixture, in a climate chamber at 25 °C, 60% RH under a 14:10 h (L: D) photoperiod.
A colony of T. evanescens was established from parasitized egg-masses collected from maize fields. The parasitoid species was identified by Dr. Viktor Fursov, the Department of Entomophagous Insects and Biocontrol, Schmalhausen Institute of Zoology of National Academy of Sciences of Ukraine, Kyiv, Ukraine. The parasitoid wasps were reared on 1-day-old E. kuehniella eggs that were previously sterilized by ultraviolet irradiation, in climate chambers at 25 °C, 70% RH under a 14:10 h (L:D) photoperiod. The 0–24-h-old sterilized eggs of E. kuehniella were glued on paper strips (1 × 8 cm) with 10% Arabic gum. The strips were placed in glass tubes (2.5 × 16 cm) and exposed to the adults of T. evanescens with 50% honey-water solution that was provided in film form as nutrient source. To maintain the colony, parasitized eggs of E. kuehniella were transferred into new glass tubes after 8 days and kept at the same conditions until emergence of the parasitoids.
Effect of different diets on the development of Ephestia kuehniella
Three different larval diets, CY diet (95% cornmeal + 5% yeast), CBGY diet (53.3% cornmeal + 26.7% wheat bran + 15% glycerine + 5% yeast), and WBGY diet (53.3% wheat flour + 26.7% wheat bran + 15% glycerine + 5% yeast), were provided to E. kuehniella larvae. All diets used in the experiments were the same with Kurtuluş et al. (2020) which provided the most promising results in terms of rearing of E. kuehniella under laboratory conditions. However, preliminary studies showed that the size of the rearing boxes can affect the fecundity and development of the moths. Therefore, the experiments were carried out in big boxes (30 × 15 × 10 cm) to unravel the efficiency of mass rearing method.
Dry matter, ash, lipid, and crude protein content of each diet are indicated in Table 1. Nitrogen percentage in the diet was calculated using Micro-Kjeldahl (AOCS 1989). The protein percentage was measured by multiplying the nitrogen percentage with a constant 6.25. Oil was extracted from the diets using Soxhlet, and the oil percentage was estimated according to the Association of Official Analytical Chemists (AOCS 1989).
One kilogram from each diet was used at all experiments. Pre-weighed diet mixtures were respectively placed in the plastic boxes (30 × 15 × 10 cm), and 3000 E. kuehniella eggs were homogeneously dispersed in each container. To determine the 3000 eggs, firstly 100 eggs were counted under a stereoscopic microscope (× 45 magnifications) (Olympus SZ51) and weighed on a precision scale (0.0001 g) (Dikomsan, FGH 200). After the calculation of the weight of the 100 eggs, it was multiplied by 30 to ensure sufficient amounts of 3000 eggs’ weight. Plastic boxes were kept in the climate chamber at 25 °C, 60% RH under 14:10 (L: D) h photoperiod. Larvae were checked daily up to pupal stage. Following the emergence of the pupae, 100 pupae (four corners and one center from the plastic boxes) were removed for each replicates with the help of soft tweezers and separated as males and females under a stereoscopic microscope at × 45, based on their external genitalia. The separated pupae were weighed individually on a precision scale (0.0001 g). Weighed pupae were transferred into plastic containers (5 × 3 × 3 cm) and placed in the same climate chamber. To determine the adult emergence, hatched pupae numbers were checked, and preimaginal development time were recorded. Experiments were carried out in a completely randomized design with 5 replicates for each diet.
Effects of different diets on the reproduction of Ephestia kuehniella
All individuals obtained from the same diet were transferred by an electronic collector into the plastic oviposition cages (30 × 20 cm, all sides windowed and covered with fine muslin to provide ventilation and oviposition site for the adult moths). For each diet, all replicates were kept in different oviposition cages and placed in a rearing room at the aforementioned conditions. Oviposition cages were checked daily, and all eggs were collected with the help of the brush and put inside the airtight plastic containers (20 cc). The collected eggs were weighed on a precision scale daily (0.0001 g). A hundred eggs were counted from the plastic containers randomly and weighted. Total weight of the eggs collected daily was divided to weight of 100 eggs. Following the determining of the daily egg number, total fecundity of E. kuehniella was detected. For the maximum benefit of the oviposition, cages were kept 4 days with the adult moths, and then, the cages were discarded.
Effects of different Ephestia kuehniella diets on the development, reproduction, and longevity of Trichogramma evanescens’ F0 and F1 generations
Newly emerged wasp adults obtained from the laboratory colony (F0) were left inside the glass tubes (2 × 10 cm) at least 12 h for copulation, and then, one female individual was transferred into a new tube. Fifty 24-h-old sterilized E. kuehniella eggs were glued on paper strips (1 × 4 cm) with 10% Arabic gum and provided to T. evanescens for 24 h. After the exposure process, the paper strips were renewed, and the older one was transferred to separated glass tube for observation of wasp’s development. Once the parasitized eggs were blackened (after 4 days), they were counted under a stereomicroscope, and following the adult emergence rate, the female and male ratio of the parasitoid was determined. Emergence rates were recorded by counting emergence holes from black eggs. Since T. evanescens can lay most of their eggs in 2 days (Achiri et al. 2020), the experiments were concluded after 48 h. To determine longevity, all alive individuals were kept unfed in the same tubes and checked 2 times a day to count dead individuals. For F1 generation experiments, following the adult emergences from the F0 experiments, different host eggs were provided to parasitoid wasp at the aforementioned conditions. The experiments were carried out for both generations in a completely randomized design, at least 10 replicates for each diet, in climate chambers at 25 °C, 70% RH under a 14:10 (L:D) h photoperiod.
Effects of different diets on the development of Ephestia kuehniella
The nutrient contents of the larval diet affect the developmental time of lepidopteran insects (Moghaddassi et al. 2019). In this study, the preimaginal developmental time was significantly shorter when larvae were fed on more complex diets, CBGY and WBGY, than the basic diet, CY (F2,12 = 11.231, p = 0.002; Table 2). Kurtuluş et al. (2020) found positive effects of the additional ingredients as shortest developmental time was obtained from most complex diets: CBGY and WBGY. This was attributed to additional ingredients that reduced the developmental time of moths. Many studies agreed with these results that the basic diets containing generally wheat or cornmeal showed longer developmental time (Locatelli et al. 2008; Kurtuluş et al. 2020), than the complex diets containing rich protein, carbohydrate, and lipid content (Moghaddassi et al. 2019; Kurtuluş et al. 2020). Not only the different larval diets can affect the developmental time but also pupal weight and emerging ratios as well. According to results obtained from this study, the mass of female and male E. kuehniella pupae from the larvae reared on the diet WBGY was significantly greater than the mass of pupae from the other diets for both sexes (females: F2,623 = 20.042, p < 0.0001; males: F2,871 = 36.407, p < 0.0001). Kurtuluş et al. (2020) noted that composite larval diets had a great impact on pupal weights of E. kuehniella, and the average female pupal weights were always higher than those of males from the same type of diet. All results were similar with this study, but the pupal weight was relatively higher than in this study. This could be related to the size of the boxes or the number of the replicates in which 500 pupae were evaluated in this study. In general, higher pupal weight is a light of evidence that can increase the fecundity of moths (Heisswolf et al. 2009). Previous studies remarked that higher pupal weight can be correlated to the lifetime fecundity (Kurtuluş et al. 2020). However, it should be also taken into consideration that the moths arising from pupae of similar weights from different larval diets showed significant differences in fecundity (Moghaddassi et al. 2019). Thus, pupal weight is not a factor that can only be explained with higher fecundity. Moreover, the number of offspring that directly reach the adult stage is also a crucial factor for the total fecundity of insects. The emergence ratios of E. kuehniella adults from either the basic or complex diets were similar in this study (F2,12 = 0.649, p = 0.540; Table 2). Many studies agreed that there were non-significant differences between emergence ratios of E. kuehniella even if the moths were reared on different larval diets (Kurtuluş et al. 2020).
Effects of different diets on the reproduction of Ephestia kuehniella
Availability of adequate nutrition is of crucial importance for successful reproduction. Many studies reported effects of diet quality and quantity on female reproduction in insects and thereby on fitness (Kurtuluş et al. 2020). In this study, the average number of eggs collected from CY and CBGY diets was significantly higher than from the WBGY diet (F2,12 = 6.854, p = 0.010; Fig. 1). It showed that cornmeal and yeast had a greater effect on the fecundity of the moths.
Lima-Filho et al. (2001) obtained less egg from the diet containing cornmeal + yeast (97.0% + 3.0%). However, this outcome could be related to the difference between the yeast ratios and larval density. Kurtuluş et al. (2020) reported that an increase in fecundity was observed with complexity of diet, while percent fertility tended to reduce. With this study, the total fecundity of all diets was lower than reported by Kurtuluş et al. (2020). For the maximum benefit of the oviposition, the cages were kept for 4 days in this study, but they counted lifetime fecundity. Moreover, there are many studies that used yeast and glycerine as an additional ingredient to increase fecundity and yielded a little higher egg numbers (Moghaddassi et al. 2019). The environmental conditions, the origin of the ingredients, and larval density could be the reasons for these differences. On the other hand, the diets that contain glycerine or honey may cause fungal infections, if kept longer than the standard generation period. In the present study, CY diet had the potential for mass-rearing of natural enemies because of its higher fecundity and easy preparation. Kurtuluş et al. (2020) suggested that the same diet could be considered as a standard diet for the rearing of parasitoids and predators.
Effect of different Ephestia kuehniella diets on the developmental time and longevity of Trichogramma evanescens
Host quality is a critical factor that can determine the developmental rate and success of parasitoids (Liu et al. 2013). For the egg parasitoids that develop inside the same host, the host represents the sole source of nutrients. Several species of factitious hosts are used worldwide for rearing Trichogramma parasitoids including: E. kuehniella, S. cerealella, C. cephalonica, and G. mellonella (Linnaeus) (Shalaby et al. 2000; Abd El-Hafez et al. 2001; Hoffmann et al. 2001). In this study, the host diets (CY, CBGY, WBGY) and combined effects of diets and generations significantly affected the developmental time of T. evanescens (diets: F2,138 = 4.236, p = 0.016; diets*generations: F2,138 = 7.760, p = 0.004, Table 3).
Many studies showed that E. kuehniella is an important factitious host for the rearing of Trichogramma parasitoids (El-Wakeil 2007; Özder and Kara 2010; Farahani et al. 2016; Moghaddassi et al. 2019). In the present study, the host diets did not affect the total developmental time of T. evanescens’ F0 generation; however, they were significantly different for F1 generation (F0: F2,40 = 0.103, p = 0.903; F1: F2,29 = 12.490, p < 0.0001; Table 4). Total developmental time was significantly longer when the parasitoids were reared on the eggs from 2 generations on WBGY diet, but the longevity did not affect from the host diets of both generations (F0: F2,40 = 0.117, p = 0.927; F1: F2,29 = 0.345, p = 0.711; Table 4).
Effect of different Ephestia kuehniella diets on the reproduction of Trichogramma evanescens
Quality and quantity of food source are an important factor that can affect parasitoids’ reproductive traits (El-Wakeil 2007; Moghaddassi et al. 2019). In the present study, the parasitization parameters of T. evanescens were not influence by different host diets (F2,138 = 2.319, p = 0.102). However, the parasitization days and combined effects of generations*parasitization days significantly affected the fecundity of T. evanescens (parasitization days: F1,138 = 52.339, p < 0001; generations*parasitization days: F1,138 = 4.519, p = 0.035, Table 5).
For both generations, the parasitoid laid more eggs when the host eggs were provided within the first 24 h (Table 6). These results are in accordance with other studies showing that T. evanescens laid more eggs in the first 24 h (Achiri et al. 2020). It is known that the mean number of eggs laid per female generally decreases over time or with the age of the parasitoid (Reznik et al. 2009). Trichogramma chilonis Ishii on eggs of C. cephalonica and T. cacoeciae on Cydia pomonella (Linnaeus, 1758) (Lepidoptera: Tortricidae) eggs showed a systematic decrease in the numbers of eggs laid per female over time (Mansour 2019). Moreover, in this study, there were non-significant differences among the parasitization parameters of both generations in the same day (F1,80 = 0.105, p = 0.905; Table 6). Yet, the parasitoids laid more eggs when exposed to eggs from CY diets for subsequent generations (F2,29 = 3.912, p = 0.031; Table 6). Nathan et al. (2006) reported no differences in parasitization rate of T. chilonis when provided with C. cephalonica eggs from adults reared on different larval diets. However, Moghaddassi et al. (2019) showed that T. brassicae laid more eggs per female in the largest eggs that were obtained from E. kuehniella rich diet for the subsequent generations. These differences could be related to the nutritional content and size of the eggs.
Emergence of the parasitoids from different parasitized host eggs during the first 24 h was greater than on the second day hatching ratios for both generations. However, for the same days, the host diets did not affect the emergence of the T. evanescens adults (F0: 1st day: F2,40 = 0.128, p = 0.881; 2nd day, F2,29 = 0.281, p = 0.756; F1: 1st day: F2,29 = 0.995, p = 0.382; 2nd day, F2,29 = 0.187, p = 0.831; Table 7).
In general, emergence rates of parasitoids were similar at the same generations. In this study, the emergence rate of parasitoids was about 85% of the parasitized eggs by all larval diets. A similar pattern of results was obtained from others (El-Wakeil 2007; Farahani et al. 2016; Moghaddassi et al. 2019; Wang et al. 2019). Nutritional quality of host impacts adult parasitoid’s fitness by increasing fecundity, host size, and offspring sex ratios (Farahani et al. 2016). For F0 generations, the female ratios were similar for 2 days, but the host diets significantly affected the female ratios of the F1 generations at the first day (F2,29 = 8.205; p = 0.002). In total, there were non-significant differences among the host diets on the female ratios in the same generations (F0: F2,40 = 1.557, p = 0.223; F1: F2,29 = 2.464, p = 0.103; Table 8). But when CY diet eggs were provided to the parasitoids, female ratios were relatively higher than the others, especially for the second generation. This could be an important finding for understanding the suitability of host for the mass-rearing of the subsequent generations of parasitoids. The number of produced females is a crucial factor in explaining the success of mass-rearing programs (El-Wakeil 2007).
Understanding the impact of host diet on host acceptance by Trichogramma spp. could improve the success of biological control programs by ensuring high efficiency of the parasitoid performance in an inundative release. In the light of overall results, CY diet provided easy preparation, shorter developmental time, higher fecundity, and relatively higher female ratios of subsequent generations of the parasitoid. For further research, the efficacy of the parasitoids and predators that are reared on the CY diet could be determined against their target hosts in the field.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
One-way analysis of variance
Generalized linear model
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The author thanks Prof. Dr. Ekrem Atakan and Dr. Tange Denis Achiri from the University of Çukurova to promote starting the experiments and for helpful comments on the manuscript. Besides, the author is grateful to Antonio Biondi (University of Catania, Italy), Antonino Cusumano (University of Palermo, Italy), and Tuğcan Alınç (University of Palermo, Italy) for reading and editing the manuscript.
The current study was not funded by any instituation.
Authors and Affiliations
Department of Plant Protection, Agricultural Faculty, University of Çukurova, Adana, Turkey
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Pehlivan, S. Role of host diet on the fitness of the egg parasitoid species, Trichogramma evanescens Westwood (Hymenoptera: Trichogrammatidae).
Egypt J Biol Pest Control31, 10 (2021). https://doi.org/10.1186/s41938-020-00353-7