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Sublethal effects of different fungal isolates on Helicoverpa armigera (Lepidoptera: Noctuidae)



Broad-spectrum pesticides and Bt crops have been used against the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), but their effect on the environment and the development of resistance are the main limitations for these control measures. The use of mycobiopesticides is an ecofriendly and efficient alternative. Fungicides have a wide distribution and a high genetic variability, which allows their screening for more virulent isolates. Therefore, the objective of this work was to evaluate the interactions of H. armigera larvae with Metarhizium anisopliae and Beauveria bassiana, on mortality, virulence, sublethal effects, and life table aspects.

Main body

The isolates that caused mortality rates of H. armigera above 80% were selected for further assays. Virulence was measured by LC50 estimates, using Probit analysis to sublethal effects, and the bootstrap procedure to estimate the biological parameters: time for development, fertility, longevity, pre-ovipositional period, egg viability, and sex ratio. The H. armigera mortality rates caused by B. bassiana and M. anisopliae ranged from 45 to 100% and from 40 to 90%, respectively. Longevity, fertility, and survival rates are affected in all treatments. The pupal weight of individuals treated with fungi was lower than in the control. Deformations were observed in H. armigera pupae developed from larvae treated with B. bassiana.


The use of sublethal concentrations of isolates of M. anisopliae and B. bassiana resulted in an adverse effect on the biological parameters of H. armigera.

Main text


The cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is one of the most important insect pests of cotton in the world. It attacks more than 200 host plants (Challa et al. 2013). Its high polyphagia, reproductive capacity, and wide geographic distribution facilitated its adaptation to different agroecosystems (Tay et al. 2013). The severity of the damages caused and the frequency of applications of chemical insecticide to control this pest have accelerated its resistance development and increased environmental problems (Yang et al. 2013). Thus, efficient alternatives for its control with less aggressiveness to the environment are necessary. Entomopathogenic fungi (EPF) are important to control insect pests from several orders, including lepidopterans (Lacey 2017). These microorganisms present high efficiency of control and have been used in bioinsecticide formulations due to their harmlessness to humans, the environment, and natural enemies (Jaronski et al. 2007).

The fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), are generalists, with a wide geographic distribution and a high frequency in soils and plants (Zimmermann 2007; Rezende et al. 2015 and Botelho et al. 2019). Selecting promising isolates to control specific insect pests is possible due to their wide distribution, high intraspecific genetic variability, and spectrum of action (Goettel et al. 2010). B. bassiana and M. anisopliae have been studied for controlling H. armigera with lethal and sublethal rates, evaluating the parameters survival rate, adult longevity, sex ratio, and fertility (Ahmad and Ansari 2013 and Jarrahi 2016).

Pest species fertility, mortality rates at different instars, and sex ratio of the population are required data to determine the pathogenicity of certain concentrations of EPF. The life table with inclusion of males increases knowledge about the population dynamics of the pests (Peroni and Hernández 2011). Therefore, interaction studies of isolates are essential to predict the effect of EPF on pest’s population.

The objective of the study was to evaluate the changes in biological parameters of H. armigera larvae treated with different isolates of B. bassiana and M. anisopliae and compare the efficiency of these pathogens.

Materials and methods

Fungal isolates

Twenty-three isolates of the fungi B. bassiana and M. anisopliae were obtained and used in the bioassays. They were isolated from the collection of EPF cultures (ARSEF-USDA) of the School of Agriculture Luiz de Queiroz (ESALQ, Piracicaba, SP, Brazil) and Biological Institute (IBCB, Campinas, SP, Brazil) (Table 1). Suspensions of each fungus used in the bioassays were prepared by scraping the conidia produced in Potato Dextrose Agar (PDA) culture medium with 0.002 μl of Tween 80®. Each fungal suspension was subjected to serial dilutions of conidia counting in a Neubauer chamber (Alves 1998), following a concentration pattern of 108 conidia ml−1 for each treatment.

Table 1 Origin of entomopathogenic fungi used in the bioassays

Conidia viability test

The conidia viability test carried out as follows: a thin layer of PDA (approximately 2.0 ml) was placed in a plate after the solidification of the medium. Each plate received 0.1 ml of the fungal suspension, which was prepared by each fungal isolate in 10 ml of sterile distilled water and 0.02% Tween 80®. The suspensions spread, using a Drigalski loop, after flamed and properly cooled. The plates were then kept in a greenhouse at 25 ± 2 °C and 12-h photophase for 24 h. The plates were examined under an optical microscope with × 400 magnification for counting of 100 conidia, germinated and non-germinated, establishing their proportion.

Helicoverpa armigera mortality test

Second-instar larvae of H. armigera were used for mortality bioassays. The population of the pest was at the 5th generation. The trials consisted of 23 treatments, 10 of which were isolates from M. anisopliae, 13 from B. bassiana, and a control. Two hundred caterpillars were used in each treatment. They are placed in polyethylene containers (18 × 30 mm), under an artificial diet (Greene et al. 1976), and then inoculated with 0.2 μl, using a 10-μl pipette. The same procedure was used at the control, but replacing the fungal suspension with distilled water plus 0.02 (v v−1) of Tween 80®. The plastic containers with the insects were closed and kept in an air-conditioned room (25 °C, RH of 70 ± 10%, and photophase of 12 h). Mortality evaluations started 3 days’ post inoculation, considering the total number of dead insects at the end of 7 days. The caterpillars were identified as dead when presented no movement to the touch of a soft bristle brush. The death of the insects due to the pathogen was confirmed as follows: the insects were washed by 70% alcohol for 10 s, rinsed in distilled water for 20 s for external decontamination, placed in plates, and left in a wet chamber for 15 days. This made it possible to observe the extrusion of the pathogen, confirming the infection. The isolates that caused deaths of H. armigera above 80% up to the 7th day of evaluation were selected for the subsequent stages of the study.

Lethal concentration (LC50) of M. anisopliae and B. bassiana for H. armigera caterpillars

Mean lethal concentration (LC50) was estimated using 2nd-instar H. armigera caterpillars that were exposed to 7 concentrations (103, 104, 105,106,107, 108, 109 conidia ml−1) of isolates previously selected in the mortality tests. One hundred caterpillars/treatment were used. The methodology for these bioassays, storage, and evaluation were the same as those used in the mortality tests.

Evaluation of sublethal effects

Fifty caterpillars that survived the exposure to LC50 concentrations of the most promising isolates (B. bassiana ARSEF 1149, ARSEF 1788, ESALQ 986, ESALQ 1288, IBCB 36, IBCB 170) (M. anisopliae ESALQ 860, ESALQ 1051) were monitored daily to assess the biological parameters: instar duration (days), caterpillar weight (mg), pupal weight (mg), sex ratio, fecundity, egg viability (%), and male and female adult longevity.

The effects of different treatments on caterpillar, pre-pupa, and pupa weights were evaluated by weighing them on an analytical balance (SHIMADZU-ATY224®) at the 7th day. Sexing the pupae was carried out according to the observations of the anal and genital openings, according to Zenker et al. (2007), with the aid of a magnifying glass (ZEIZZ-STEMI 508®). The males were separated from females, and couples were formed to estimate the adult pre-ovipositional period (APOP) and total ovipositional period (TPOP). The formed couples were kept at the same conditions as they reared. Ovipositional period and longevity were recorded daily. Raw data of time for development, survival, daily fertility, and male and female longevity were used for the analysis of biological variables of H. armigera and development of the life table. All tested individuals were considered for these evaluations, including those who died during the development of immature stages (Chi and Liu 1985 and Chi 1988). Parameters of intrinsic population growth rate (r), finite population growth rate (λ), net reproduction rate (Ro), average generation duration (T), and gross reproduction rate (GRR) were estimated by the Chi and Liu’s methodology (1985), using the equations:

$$ \sum \limits_{X=0}^{\infty }\ {e}^{-r\left(x+1\right)}{l}_x{m}_x=1 $$
$$ lx={\sum}_{f=1}^{\beta }{\displaystyle \begin{array}{c} Sxj\\ {}\ \end{array}} $$
$$ mx=\frac{\sum_{j=1}^{\beta } Sxj fxj}{\sum_{j=1}^{\beta } Sxj} $$

where β is the number of life stages; sxj is the age-specific survival rate, considering the difference and standard deviation; lx is the age-specific survival rate; fxj is the age-specific fertility of the stage; and mx is the age-specific fertility.

Statistical analysis

A completely randomized design with 24 treatments and 200 replications was used to select the isolates; each caterpillar was a replication. Statistical analysis was performed using the SAS program (University 2002); when the means were significant, they were compared by the Tukey test at 5% probability. The mortality data found for the different concentrations were subjected to Probit analysis, using the POLO PC Software (Leora-software 1987) to estimate the LC50.

Regarding the sublethal effects, the bootstrap procedure was used to estimate the variations, means, and standard error of the biological parameters: time for development, fertility, and longevity, using 100,000 bootstraps. The parameters of the H. armigera populations under fungal treatments and the control were compared by the paired bootstrap test (p < 0.05), based on the differences in confidence interval (CI = 95%) using the TWOSEX-MS-Chart program (Chi 2016). Hierarchical cluster analysis was used, with the Euclidean dissimilarity measured by the coefficient similarities. Ward’s clustering method was used to identify the similarity between groups. Non-hierarchical cluster analysis was used by the K-means method, with k corresponding to the number of groups indicated in the hierarchical cluster analysis. The data of the variables were processed in the R program (R Core Team, 2018).

Results and discussion

Mortality rates

Mortality rates of 2nd-instar H. armigera caterpillars caused by the isolates of M. anisopliae and B. bassiana were different. The highest means were found at the treatments of IBCB 425 and ESALQ 860 for M. anisopliae, and IBCB 1363 and IBCB 36 of B. bassiana (Fig. 1). The number of highly virulent isolates against H. armigera was high at the fungus B. bassiana, corroborating the results of Hassani (2000), who tested the virulence of the fungus B. bassiana against H. armigera and Spodoptera littoralis caterpillars and found that several isolates of B. bassiana were highly virulent against noctuid species.

Fig. 1

Mortality rates of second-instar Helicoverpa armigera caterpillars caused by Metarhizium anisopliae and Beauveria bassiana, compared by Tukey’s test at 5% probability

Conidial viability

Conidial viability of the isolates ranged 42.0–98.0% at the concentration between 1 × 102 and 1 × 108, respectively. The isolates ARSEF 1149, ESALQ 1288, IBCB 36, IBCB 170, and IBCB 1363 of the B. bassiana fungus presented a high virulence and the highest numbers of viable conidia. The isolates ESALQ 860, ESALQ 1051, and IBCB 425 of M. anisopliae fungus had the highest number of viable conidia, differing from the other isolates, presenting the same virulence in the mortality test (Fig. 1). The 8 isolates that caused deaths of H. armigera above 80% were used in the tests to estimate LC50.

LC50 estimates (Table 2)

Table 2 Estimates of LC50 of Beauveria bassiana and Metarhizium anisopliae isolates for second-instar Helicoverpa armigera larvae, using the Polo-PC program

H. armigera longevity and fecundity

Ovipositional period was affected in 2 M. anisopliae isolates—ESALQ 860 (3.59 days) and ESALQ 10.51 (4.2 days). The control presented 5.2 days, but with non-significant difference (Tables 3 and 4), as shown by the paired bootstrap method. Total time for development in the control (46.66 days) was shorter than that at the treatments ESALQ 860 (48.88 days) and ESALQ 1051 (58.8 days), but did not differ from that of the isolate IBCB 425 (47.7 days). A high susceptibility of H. armigera larvae to B. bassiana isolates was found and also observed by Nguyen et al. (2007) and Kalvnadi et al. (2018). However, the results of larval developmental period differed from those presented by Nguyen et al. (2007).

Table 3 Developmental periods (days) of different stages, longevity, fecundity, pre-ovipositional, and total ovipositional period of Helicoverpa armigera caterpillars infected with different isolates of Metarhizium anisopliae, and a control (untreated)
Table 4 Developmental periods (days) of different stages, longevity, fecundity, pre-ovipositional, and total ovipositional period of Helicoverpa armigera caterpillars infected with different isolates of Beauveria bassiana, and a control (untreated)

The total pre-ovipositional period (TPOP) in the treatments of ESALQ 860 and ESALQ 1051 was significantly longer than that in the control (38.52 days), but did not differ from that of the IBCB 425 treatment. The adult pre-ovipositional period (APOP) in the treatments was similar to that in the control. The average fertility of females in all treatments was lower than in the control. The lowest egg viability was found at the isolate IBCB 425 (60.14%) (Table 3).

The ovipositional duration of caterpillars exposed to B. bassiana isolates was different from that in the control (5.2 days) (Table 4), but similar to those of the other isolates. The developmental periods of the larvae and pupae of the isolates were different and were affected in all treatments than in the control. Strategies to avoid or overcome the actions of the pathogens are different. Each host population evolves and presents the different immune responses (Lu et al. 2016). The isolates may have intrinsic characteristics, which related to virulence. One of these characteristics is the production of extracellular enzymes; the production of enzymes can be different depending on the isolate and present temporal variations (Wang et al. 2009).

The maintenance of this virulence above the levels of the enzyme production may also be related to the genetic diversity of these fungi, which may be influenced by the habitat in which each isolate was obtained (Mora et al. 2016). A research comparing the location and isolation method showed that fungi isolated from soil resulted to a high mortality rate of H. armigera caterpillars (Kalvnadi et al. 2018). In the present study, the highest virulence was found for caterpillars exposed to isolates from soil; the isolate IBCB 1363, for example, was isolated from the soil of a rubber tree plantation. However, M. anisopliae isolates showed non-effect of the habitat.

Adult longevity is affected by the treatments of both sexes. It was shorter at some isolates and longer in others than the control (45.00 for females and 48.07 for males). The female longevity decreased at B. bassiana isolates, consistent with the results of Wu et al. (2014), and increased at M. anisopliae isolates, differing from the results found by Jarrahi and Safavi (2016). Longevity was low in 3 treatments IBCB 36 (35.16 days), IBCB 170 (29.46 days), and IBCB 1363 (42.78 days), differing significantly from the control (46.66 days). The isolates ARSEF 1149 (47.72 days) and ESALQ 1288 (47.08 days) did not differ from the control (Table 4). TPOP was affected by the most treatments; it was short at the isolate IBCB 36 (30.00), long at the isolates ESALQ 1288 (48.88), and similar at the isolates IBCB 170 (40.36), IBCB 1363 (37.53), and ARSEF 1149 (37.45), when compared to the control (38.52).

The APOP in the control was 2.70 days, similar to those of the isolates ARSEF 1149 (2.45), IBCB 170 (2.36), and IBCB 1363 (2.53). The APOP of the ESALQ 1288 (3.44) and IBCB 36 (3.00) was significantly different from those of the other isolates. The average fertility of females in all treatments was lower than that in the control (470.83), but did not differ from each other at the isolates IBCB 1363 (328.27), ARSEF 1149 (221.23), ESALQ 1288 (338.12), and IBCB 1363 (328.27). The egg viability was low, mainly at the isolates IBCB 1363 (75.24%) (Table 4).

Jarrahi and Safavi (2016) found different results than Arthurs and Thomas (2000), who reported that the adult pre-ovipositional period and total ovipositional period extended in H. armigera at M. anisopliae. The egg viability of females treated with the isolates was low, as found by Kalvnadi et al. (2018); however, it differed from the results of Arthurs and Thomas (2000), who found no reduction in fertility of adults treated with M. anisopliae.

Grouping of parameters

Complementing the results of the bootstrap analysis, hierarchical and non-hierarchical classifications were performed for each fungal isolate. The hierarchical cluster analysis, represented by a dendrogram, formed 4 groups: group 1 was formed by the ESALQ 1051 isolate, which was characterized by a lower mortality rate, and greater caterpillar longevity and pupa and total pre-ovipositional periods. Group 2 was formed by IBCB 36, characterized by a low egg, pupa, caterpillar, fecundity, and oviposition. Group 3 was formed by ESALQ 1288 and IBCB 425, and group 4 was formed by ARSEF 1149, IBCB 1363, ESALQ 860, and IBCB 170, characterized by average values for all parameters (Figs. 2 and 3). The results described were confirmed by the clustering analysis (Figs. 2 and 3); groups were formed despite the isolates had different characteristics. The differences among isolates tend to be lower when considering all parameters of the sublethal effect, promoting an effective control for H. armigera.

Fig. 2

Dendrogram of the hierarchical cluster analysis for the ordering of fungal isolates

Fig. 3

Non-hierarchical cluster analysis (K-means) for the ordering of fungal isolates. Mortality (MTD), egg stage duration (LOGOV), caterpillar longevity (LOGLA), pre-pupa longevity (PRPU), pupa stage duration (PUPA), total longevity (LOGTO), adult pre-oviposition period (APOP), total pre-oviposition period (TPOP), fertility (FECU), oviposition (OVI)

Principal component analysis

The groups obtained in the previous analysis were confirmed by subjecting the results to principal component analysis (Fig. 4). The eigenvalues of the principal components (PC) PC1 and PC2 explained 70% of the response variation of H. armigera caterpillars to the exposure to fungal isolates. Therefore, the total amount of information contained in the evaluated parameters formed 4 distinct groups, which were similar to those observed in the hierarchical cluster analysis. PC1 explained 45.45% of the variance (eigenvalue of 4.54); the parameters with greater discriminatory power in PC1 were BAT (0.76), LOGOV (− 0.65), LOGLA − 0.78), PUPA (− 0.86), LOGTO (− 0.95), and TPOP (− 0.88). PC2 explained 24.51% of the variance (eigenvalue of 2.45); the parameters with the greatest discriminatory power were PRPU (0.68), FECU (0.90), and OVI (0.69). The biplot graph (Fig. 4) shows the distribution of lethal and sublethal parameters of H. armigera caterpillars exposed to fungal isolates. The figure shows the parameters that best discriminate each isolate.

Fig. 4

Biplot graph for distribution of fungal isolates and variables in the first two main components

The positive correlations discriminated the isolates at the right of PC1 (IBCB 425, ESALQ 1288, IBCB 1363, and IBCB 36), and the negative correlation discriminated the isolates at the left of PC1 (ESALQ 1051, ESALQ 860, ARSEF 1149, and IBCB 170) (Fig. 4). The variables with positive correlation discriminated the isolates in the upper part of PC2 (IBCB 425 and ESALQ 1288), and the variables with negative correlation discriminated the isolates in the lowest part of PC2 (ESALQ 1051, ESALQ 860, ARSEF 1149, IBCB 170, IBCB 1363, and IBCB 36). According to the results for the variables of the isolates, the isolates IBCB 425 and ESALQ 1288 potentially had a great pre-pupa weight, adult pre-ovipositional period, and mortality, and the isolates ESALQ 1051, ESALQ 860, ARSEF 1149, and IBCB 170 had a high pupal weight and caterpillar longevity.

Sex ratio and caterpillar and pupa weights

Effect of B. bassiana on biological parameters of H. armigera

The weight of caterpillars after 10 days of exposure to the treatments was lower than that in the control, with differences among treatments. The pre-pupal and pupal weights of the isolate IBCB 1363 were affected, with consequent abnormal pupae and defective adults (Fig. 5).

Fig. 5

Morphological abnormalities in Helicoverpa armigera treated with LC50 of the fungus B. bassiana (isolate IBCB1363). a Interference in the development of caterpillars shown by the difference in size. b Early pupal phase. c Low pupal weight and size. d Pupal and adult deformations

Fungi act via tegument, which is an advantage over other groups of pathogens that only enter the insects orally. This contact action is due to the secreting of a series of compounds, including bassianin, bassiacridin, oosporeins, cyclosporine, and destruxins (Molnar et al. 2010; Wang et al. 2010 and Gibson et al. 2014), which were dependent on the specificity of the host and can suppress their immune response (Amiri-Besheli et al. 2000).

The exoskeleton and digestive tract of insects are barriers that can be broken and trigger several cellular and hormonal mechanisms, resulting in an immune response (Levy et al. 2004). During these responses, insects begin to lose lipids and proteins that are essential to complete their cycle (Qu and Wang 2018), explaining their weight loss at the pupal stage and the non-emergence of adults found in the present study.

Kaur et al. (2011) reported morphological abnormalities in Spodoptera litura, using EPF. Most caterpillars treated with B. bassiana isolates were unable to complete their larval phase, and extending this stage and early forming pupae. This is explained as the actions of enzymes, and the insects’ immune system, which accelerate the molting process, reducing the production of youth hormone and maintaining the production of ecdysone, which induce the ecdysis process (Qu and Wang 2018). The concentrations of these hormones in the hemolymph determine the completion of metamorphosis and the reproductive process of insects, which may also explain the decrease in fertility of adults treated with the isolates when compared to the control (Rohlfs and Churchill 2011).

Survival decreased considerably at the pupal stage, i.e., surviving caterpillars reached the pupal stage with a decrease weight and morphological malformations that caused their death (Fig. 5). These morphological deformations are explained by the insect defense system, which is composed of structural barriers and active responses against external elements that reach the hemocoel, such as EPF (Renwrantz 1986). The sex ratio is not affected by any of the treatments (Table 5).

Table 5 Sex ratio, number of eggs per female, egg viability, caterpillar weight, pre-pup weight (g), and pupa weight (g) of Helicoverpa armigera treated with Beauveria bassiana and Metarhizium anisopliae

Effect of the M. anisopliae on biological parameters of H. armigera

The caterpillar weight after 10 days of application of the treatments was lower than that in the control for all treatments. The pre-pupal and pupal weights of the ESALQ 860 and ESALQ 1051 isolates differed than the control, but were similar to each other. The IBCB 425 isolate did not differ from the control, but differed from the other isolates. The effect of the treatments on the sex ratio was non-significant (Table 5).

Life table

The average generation time (T) of H. armigera treated with the B. bassiana isolates ARSEF 1149, reached 40.03 days, and ESALQ 1288 (51.07 days) had a significant difference than the control (41.29 days). The net reproduction rate (Ro) in the control was 216.58. It was 2.5% higher than those in the treatments (Table 6).

Table 6 Mean parameters (± SE) of the Helicoverpa armigera life table subjected to different isolates of the fungi Beauveria bassiana and Metarhizium anisopliae over one generation at 25 ± 1 °C and 70 ± 10% RH

The intrinsic rate of increase (r) in the control (0.130 day−1) was significantly higher than those at 4 isolates. Only the isolate IBCB 36 showed no difference than the control. The finite rate of increase (ƛ) in the treatments showed a non-significant difference. The gross reproduction rate (GRR) of H. armigera in the control (248.61) was higher than those of all isolates tested, showing a significant difference between females at all treatments (Table 6). All M. anisopliae isolates affected the average generation time (T), which was significantly higher than in the control. The net reproduction rate (Ro) of the treatments was lower than that in the control. The intrinsic rate of increase (r) in the control (0.130) was higher than that in the treatments, but the finite rate of increase (ƛ) showed no difference for any isolate tested. The gross reproduction rate (GRR) in the control (248.61) was higher than that in the treatments IBCB 425 (205.23), ESALQ 860 (136.45), and ESALQ 1051 (113.35) (Table 6).

Beauveria bassiana and M. anisopliae affected the biological parameters T, Ro, r, ƛ, and GRR of H. armigera, but with lower intensity than the control, and depending on the isolate. The net reproduction rate (Ro) was significantly lower than in the control, promoting effects that will compromise the subsequent generation, acting effectively in the control of the species.


The use of sublethal concentrations of the fungi isolates of M. anisopliae and B. bassiana resulted in an adverse effect on the biology of H. armigera. The high number of promising fungus isolates increases the probability of an effective control of these species by these fungi and reduction in the development of resistance in H. armigera populations.

Availability of data and materials

All data of the study have been presented in the manuscript, and high-quality and grade materials were used in this study.



Bacillus thuringiensis


Collection of Entomopathogenic Fungal Cultures


School of Agriculture Luiz de Queiroz


Biological Institute of Campinas


Potato Dextrose Agar


Lethal concentration


Period from the appearance of adults to the first oviposition


Total pre-oviposition

r :

Population growth rate

λ :

Finite population growth rate

R o :

Net reproduction rate

T :

Average generation duration


Reproduction rate

Sxj :

Age-specific survival rate

Fxj :

Age-specific fertility of the stage

mx :

Age-specific fertility




Egg stage duration


Caterpillar longevity


Pre-pupa longevity


Pupa stage duration


Total longevity






Principal components


  1. Ahmad S, Ansari MS (2013) Acute toxicity and sub-lethal effects of a pyrethroid (Cypermethrin) on survival, development and fitness of Helicoverpa armigera. Archives of Phytopathology and Plant Protection 46:1726–1739

    CAS  Article  Google Scholar 

  2. Alves, SB (1998) Controle microbiano. FEALQ, Piracicaba, São Paulo, 1163p.

  3. Amiri-besheli B, Khambay B, Cameron S, Deadman ML, Butt TM (2000) Inter and intra specific variation in destruxin production by insect pathogenic Metarhizium spp., and its significance to pathogenesis. Mycological Research 104:447–452.

    CAS  Article  Google Scholar 

  4. Arthurs S, Thomas MB (2000) Effects of a mycoinsecticide on feeding and fecundity of the Brown locust Locustana pardalina. Biocontrol Science Technology 10:321–329

    Article  Google Scholar 

  5. Botelho RZ, Alves PAB, Colonhez PA, Zucchi RMI, Delalibera JI (2019) Metarhizium species in soil from Brazilian biomes: a study of diversity, distribution, and association with natural and agricultural environments. Fungal Ecology 41:289–300

    Article  Google Scholar 

  6. Challa MM, Sanivada SK, Koduru UD (2013) Total soluble protein profiles of Beauveria bassiana and their relationship with virulence against Helicoverpa armigera. Biocontrol Science and Technology 23:1169–1185

    Article  Google Scholar 

  7. Chi H (1988) Life-table analysis incorporating both sexes and variable development rate among individuals. Journal Environ Entomol 17:26–34

    Article  Google Scholar 

  8. Chi H (2016) TWOSEX-MSChart: a computer program for the age-stage, two-sex life table analysis. http://140.120.197 .173/Ecology/Download/Twos ex-MSChart. zip acessado 10 setembro 2019.

  9. Chi H, Liu H (1985) Two new method for the study of insect population ecology. Bulletin 24:225–240

    Google Scholar 

  10. Gibson DM, Donzelli BG, Krasnoff SB, Keyhani NO (2014) Discovering the secondary metabolite potential encoded within entomopathogenic fungi. Natural Product Reports 31:1287–1305.

    CAS  Article  PubMed  Google Scholar 

  11. Goettel MS, Elienberg J, Glare T (2010) Entomopathogenic fungi and their role in regulation of insect populations. In: Laurence IG, Sarjeet SG (Eds). Insect control: biological and synthetic Agents 113:206–2014

    Google Scholar 

  12. Greene GL, Leppla NC, Dickerson WA (1976) Velvetbean caterpillar: a rearing procedure and artificial diet. Journal of Economic Entomology 69:497–488

    Article  Google Scholar 

  13. Hassani M (2000) Development and proving of biocontrol methods based on Bacillus thuringiensis and entomopathogenic fungi against the cotton pests Spodoptera littoralis, Helicoverpa armigera (Lepidoptera: Noctuidae) and Aphis gossypii (Homoptera: Aphididae). 130 f. TESE. University of Giessen, Germany.

  14. Jaronski ST (2007) Soil esology of the entomopathogenic Ascomycetes: a critical examination of what we (think) we know. In: Ekesi S, Maniania NK Use of Entomopathogenic Fungi in Biological. Pest Management 1:91–144

    Google Scholar 

  15. Jarrahi A, Safavi SA (2016) Fitness costs to Helicoverpa armigera after exposure to sub-lethal concentrations of Metarhizium anisopliae sensu lato: study on F1 generation. Journal of Invertebrate Pathology 138:50–56

    Article  Google Scholar 

  16. Kalvnadi E, Mirmoayedi A, Alizadeh M, Pourian HR (2018) Sub-lethal concentrations of the entomopathogenic fungus, Beauveria bassiana increase fitness costs of Helicoverpa armigera (Lepidoptera: Noctuidae) offspring. Journal of Invertebrate Pathology 158:32–42.

    Article  PubMed  Google Scholar 

  17. Kaur, S.; Kaur, H.P.; Kaur, K.; Kaur, A. Effect of different concentrations of Beauveria bassiana on development and reproductive potential of Spodoptera litura (Fabricius). Journal of Biopesticides, v. 4, p. 161–168, 2011.

  18. Lacey AW (2017) Microbial control insect and mite pests. FNP: Academic Press, 446p.

  19. Leora Software, polo-pc (1987) a user’s guide to Probit or Logit analysis. Leora Software, Berkeley, CA.

  20. Levy SM, Falleiros AMF, Gregório EA, Arrebola NR, Toledo LA (2004) The larval midgut of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae): light and electron microscopy studies of the epithelial cells. Brazilian Journal of Biology 64:633–638

    CAS  Article  Google Scholar 

  21. Molnar I, Gibson DM, Krasnoff SB (2010) Secondary metabolites from entomopathogenic Hypocrealean fungi. Natural Product Reports 27:1241-1275. 10.1039/c001459c

  22. Mora MAE, Rouws CRJ, Fraga EM (2016) Occurrence of entomopathogenic fungi in Atlantic forest soils. Microbiology Descubray 4:2052-6180. http://dx.doi.10.7243/2052-6180-4-1

  23. Nguyen NTH, Borgemeister C, Poehling MH, Zimmermann G (2007) Laboratory investigations on the potential of entomopathogenic fungi for biocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) larvae and pupae. Biocontrol Science and Technology17: 853-864

  24. Peroni N, Hernández M (2011) Ecologia de Populações e Comunidades. ISCED (ED), 185p.

  25. Qu S, Wang S (2018) Interaction of entomopathogenic fungi with the host immune system. Developmental e Comparative Immunology 83:96–103

    CAS  Article  Google Scholar 

  26. R Core Team (2018). R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria

  27. Renwrantz L (1986) Lectins in molluscs and arthropods: their occurrence origin and roles in immunity. In: Lackie, AM (Ed). Immunity mechanisms in invertebrate vectors 150p.

  28. Rezende LM, Zanardo ABR, Lopes MS, Delalibera JRI, Rehner AS (2015) Phylogenetic diversity of Brazilian Metarhizium associated with sugarcane agriculture. BioControl 60:495–505.

    Article  Google Scholar 

  29. Rohlfs M, Churchill ACL (2011) Fungal secondary metabolites as modulators of interactions with insects and other arthropods. Fungal Genetics and Biology 48:23–34.

    CAS  Article  PubMed  Google Scholar 

  30. Tay WT, Soria FM, Walsh T, Thomazoni D, Silvie P, Behere TG, Anderson C (2013) A brave new world for an old-world pest: Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil. Plos One 8:134–138

    Google Scholar 

  31. Wang D, Qiu X, Ren X, Niu F, Wang K (2009) Resistance selection and biochemical characterization of spinosad resistance in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). Pesticide Biochemistry and Physiology 95:90–94

    CAS  Article  Google Scholar 

  32. Wang Z, Wilhelmsson C, Hyrsl P, Loof TG, Dobes P, Klupp M, Loseva O, Morgelin M, Ikle J, Cripps RM, Herwald H, Theopold U (2010) Pathogen entrapment by transglutaminase-a conserved early innate immune mechanism. PLoS Pathog 6:1–10

    Google Scholar 

  33. Wu S, Youngman RR, Kok LT, Laub CA, Pfeiffer DG (2014) Interaction between entomopathogenic nematodes and entomopathogenic fungi applied to third instar southern masked chafer white grubs, Cyclocephala lurida (Coleoptera: Scarabaeidae), under laboratory and greenhouse conditions. Biological Control, 76:65–73. doi:10.1016/j.biocontrol.2014.05.002

  34. Yang Y, Li Y, Wu Y (2013) Current status of insecticide resistance in Helicoverpa armigera after 15 years of Bt cotton planting in China. Journal of Econmic Entomology 1061:375–381.

    Article  Google Scholar 

  35. Zenker MM, Specht A, Corseuil E (2007) Estágios imaturos de Spodoptera cosmioides (Walker) (Lepidoptera, Noctuidae). Revista Brasileira Zoologia 24:99–107

    Article  Google Scholar 

  36. Zimmermann G (2007) Review on safety of the entomopathogenic fungus Metarhizium anisopliae. Biocontrol Science and technology 17:879–920

    Article  Google Scholar 

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The authors thank the Coordination for the Improvement of Higher Education Personnel - Brazil (CAPES) for granting the scholarship; the State University, Jaboticabal, São Paulo, for the infrastructure; the Collection of Entomopathogenic Fungal Cultures (ARSEF-USDA) of the School of Agriculture Luiz de Queiroz (ESALQ, Piracicaba, SP, Brazil); and the Campinas Biological Institute (IBCB, Campinas, SP, Brazil). This study was partly financed by the CAPES (Financial Code 001).


The work was financed by the São Paulo State Research Support Foundation (FAPESP, grant n° 2018 / 21089-3), which subsidized all purchases of inputs and materials used, together with the support of CAPES for the aid to the student scholarship (Financial Code 001).

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TDS: Master’s student responsible for the project, participated in the planning and execution of bioassays, data analysis, and writing of the manuscript. FOF: Assisted in the setup and evaluation of experiments in the laboratory and in the analysis of the data. ACS: Assisted in the setup of the experiments in the laboratory. RAP: Project supervisor; guided the data collection and reviewed the manuscript. All authors have read and approved the manuscript.

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Correspondence to Tamires Doroteo de Souza.

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de Souza, T.D., Fernandes, F.O., Sanches, A.C. et al. Sublethal effects of different fungal isolates on Helicoverpa armigera (Lepidoptera: Noctuidae). Egypt J Biol Pest Control 30, 141 (2020).

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  • Helicoverpa armigera
  • Metarhizium anisopliae
  • Beauveria bassiana
  • Pathogenicity
  • Sublethal effects
  • Life table