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Efficacy of some local isolates of the fungus Beauveria bassiana (Balsamo) Vuillemin on the alfalfa weevil Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) larvae, under laboratory conditions


Control potential of 10 Beauveria bassiana isolates, isolated from Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) and Gonioctena fornicata (Brüggemann) (Coleoptera: Chrysomelidae), collected from alfalfa fields in Tokat province, Turkey, was evaluated against H. postica larvae under laboratory conditions. Concentration-response tests were carried out using the concentrations (1 × 103, 1 × 105, 1 × 107, and 1 × 109 conidia/ml) of isolates GN-23, GN-4, HP-30, and HP-6, which performed more than 95% efficacy in screening tests (1 × 107 conidia/ml) 5 days post treatments. LT50 and LT90 values at 1 × 109 conidia/ml were determined. According to the obtained results, H. postica larvae were susceptible to all the tested B. bassiana isolates.


Alfalfa (Medicago sativa L.) is one of the well-known and widely used forage crops in the world. Its high yield and quality allows it to be used in feeding programs of different types of livestock (Bates 1998). Alfalfa is attacked by a numerous number of insect species that cause considerable damage and reduce forage yield. Alfalfa weevil Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) is one of the most important pests that attack this crop. Both adults and larvae feed on alfalfa foliage, but the larvae cause the majority of the damage. Larvae feed initially on the inside of terminal leaves and later move to the lower portions of the plant, while adults generally feed on the leaf margins. Injured leaves dry very quickly giving the field a grayish to whitish cast. This pest can be found in all alfalfa production areas of Turkey and worldwide (Cook et al. 2004 and Atanasova 2012).

Frequent cuttings are the most effective cultural method of the pest in the alfalfa fields. Chemical control can be recommended, if there are 25 adults in 1-m2 area before the first mowing (Erdoğan 2008). However, the chemical insecticides used to manage this pest are extremely hazardous to bees and other beneficial insects (Reddy et al. 2016). Some efficient biological control agents, particularly entomopathogenic fungi (EPF), and some hymenopteran parasitoids have been recorded (Atay et al. 2015; Reddy et al. 2016; Efil 2018 and Yücel et al. 2018).

The application of EPF in bio-control of insects is of immense significance because of their environmental and food safety concerns (Reddy et al. 2016). Species of the genera Beauveria, Metarhizium, Lecanicillium and Isaria are commercially produced (Vega et al. 2009). B. bassiana was reported to infect 707 species of insect hosts, including 521 genera and 149 families of 15 orders (Imoulan et al. 2017). Some studies have been conducted to determine the potential of B. bassiana as a bio-agent against various insect pests in Turkey. These studies have mostly focused on lepidopteran and coleopteran pests (Güven et al. 2015; Yanar et al. 2017).

There are several studies concerning biological control of H. postica by EPF in Turkey (Atay et al. 2015 and Yücel et al. 2018) and in the world (Hedlund and Pass 1968; Roberts et al. 1994; Mustafa et al. 2014 and Reddy et al. 2016).

The objective of this study was to determine the efficacy of 10 local B. bassiana isolates, isolated from H. postica and Gonioctena fornicata, collected from alfalfa fields in Tokat province, Turkey, against larvae of the alfalfa weevil under laboratory conditions.


Isolation of fungi

Overwintered adults of H. postica and G. fornicata emerged from the soil were collected from alfalfa fields of Tokat, Turkey, during the period, April–May 2015 (Table 1). The collections were brought to the laboratory with fresh alfalfa plants, transferred to separate cages and checked daily. The dead adults were subjected to surface sterilization with 1% sodium hypochlorite solution for 1 min, washed twice with distilled water, placed in a sterile Petri-dish containing moistened filter paper and kept at 25 ± 2 °C and 16L: 8D h photoperiod (Ali-Shtayeh et al. 2002). By the end of incubation period, the fungus was isolated from the adults, with external fungal growth, with special care (Sevim 2010). PDAY (PDA + 1% yeast extract) media were used for isolation. To prevent bacterial contamination, 50 μg/ml ampicillin, 20 μg/ml tetracycline, and 200 μg/ml streptomycin were added to the medium (Ihara et al. 2001). Single-spore isolates of all the isolates were obtained by serial dilution (Dhingra and Sinclair 1995) and were identified as B. bassiana. Totally, ten B. bassiana isolates were isolated from the field-collections of H. postica and G. fornicata adults (Table 1), and they were deposited in the fungal culture collection of the Mycology Laboratory at the Gaziosmanpasa University, Faculty of Agriculture, Department of Plant Protection in Tokat, Turkey. In order to obtain sufficient amounts of spore suspensions, the fungi were sub-cultured in PDA (Potato Dextrose Agar) medium. The fungi cultures were incubated at 25 ± 2 °C for 17 days. Ten milliliters of sterilized water with 0.02% Tween 80 was added to each plate, and spore harvesting was done by gently rubbing the culture surface, using a sterilized glass hokey. Spore suspension from each isolate was adjusted to 1 × 103, 1 × 105, 1 × 107, and 1 × 109 conidia/ml (Şahin 2006).

Table 1 Hosts and locations of the tested enthomopathogenic Beauveria bassiana isolates


Primarily, screening tests were conducted to determine the efficacy of the isolates against H. postica larvae at 1 × 107 conidia/ml. To test the effect of each isolate, H. postica larvae were dipped into conidial suspension of 1 × 107 conidia/ml of each isolate for 4–5 s and placed in a Petri-dish (10 larvae per dish) containing fresh alfalfa leaves. Mortality rates were recorded on the 1st, 3rd, 5th, and 7th days post treatment. In addition, concentration-response tests were carried out with isolates proved to have a high effect, using such tested concentrations. The experiments were carried out in completely randomized block design, with three replications and replicated two times.

Statistical analysis

Test results were converted into percentages and arcsine transformed. The transformed data was analyzed by analysis of variance (ANOVA) and the means compared by Tukey’s multiple comparison tests. All statistical analyses were carried out using the MINITAB Release 16 packet program. LT50 and LT90 values of the concentration causing the fastest effect were determined, using the probit analysis.

Results and discussion

The ten EPF isolates tested against H. postica at 1 × 107conidia/ml caused 100% mortality 7 days post treatment in almost all isolates (Table 2). Concentration-response tests, carried out using concentrations of 1 × 103, 1 × 105, 1 × 107, and 1 × 109 conidia/ml of the isolates (GN-23, GN-4, HP-30, HP-6), caused more than 95% mortality rate on the 5th day.

Table 2 Mortality of Hypera postica exposed to the ten isolates at 1x107conidia/ml

The mortality rates of H. postica larvae varied from 13.66 to 72.08% 3 days post treatment. The highest mortality rate (72.08%) was recorded for HP-6 at 1 × 109 conidia/ml (Table 6). HP-30 was the most effective isolate with mortality rate of 62.22% at 1 × 103 conidia/ml (Table 5). On the 5th day, all isolates and concentrations caused more than 70% mortality, while on the 7th day the mortality rate reached 100% by almost all isolates at 1 × 107 and 1 × 109 conidia/ml (Tables 3, 4, 5, and 6).

Table 3 Mortality of Hypera postica exposed to GN-23 isolate
Table 4 Mortality of Hypera postica exposed to GN-4 isolate
Table 5 Mortality of Hypera postica exposed to Hp-30 isolate
Table 6 Mortality of Hypera postica exposed to Hp-6 isolate

LT50 for HP-30 was 2.401 days, followed by HP-6 (2.476 days), GN-23 (3.074 days), and GN-4 (3.110 days). LT90 values for the isolates of HP-6, HP-30, GN-4, and GN-23 were 3.608, 4.196, 4.343, and 4.376 days, respectively (Table 7).

Table 7 Lethal time (LT50 and LT90) values of the entomopathogenic fungi, Beauveria bassiana isolates (day)

Yücel et al. (2018) tested 7 isolates of B. bassiana and one isolate of B. pseudobassiana, isolated from H. postica, on larvae and adults of H. postica at 1 × 105, 1 × 106 1 × 107, and 1 × 108 conidia/ml. The results showed that the highest mortality rate in larvae was obtained by the isolate HpA-5 (B. bassiana) (100%) and HpI-4 (B. pseudobassiana) (97%), within 14 days at 1 × 108 conidia/ml. The highest mortality rate of adults were obtained by the isolates HpA-5 (B. bassiana) and HpI-4 (B. pseudobassiana) with 98 and 95% mortality rates within 14 days at 1 × 108 concentration, respectively.

Mustafa et al. (2014) reported that conidial suspension with 1 × 107 conidia/ml of two isolates of B. bassiana caused 100% mortality on adults of H. postica, 6 days post treatments. Also, Reddy et al. (2016) investigated the efficiency of six biorational-insecticides against H. postica larvae under laboratory conditions and found that Mycotrol® ESO (B. bassiana GHA) lasted 5–9 days to kill 100% of H. postica larvae in all the tested concentrations (0.072, 0.36, 0.72, and 1.44 ml/l). In addition, Atay et al. (2015) stated that 23.25% of H. postica adults, overwintered in alfalfa growing areas of Tokat province in Turkey, were found naturally infected with Beauveria spp. Harcourt et al. (1977) reported that larvae of this pest were found infected with Entomophthora phytonomi Arthur, which considerably reduced the weevil population in Canada.


The present study showed that the local isolates of B. bassiana could be suggested as bio-control agent against H. postica larvae; however, further studies should be conducted under field conditions.


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The study was supported by Gaziosmanpaşa University Scientific Research Fund (project number: 2015/138).

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Correspondence to Turgut Atay.

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Baysal, E., Atay, T. & Yanar, Y. Efficacy of some local isolates of the fungus Beauveria bassiana (Balsamo) Vuillemin on the alfalfa weevil Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) larvae, under laboratory conditions. Egypt J Biol Pest Control 28, 65 (2018).

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