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Biological control potential of North West Himalayan strains of heterorhabditid nematodes against the turnip moth, Agrotis segetum (Denis & Schiffermuller) (Lepidoptera: Noctuidae)

Egyptian Journal of Biological Pest Control201828:37

Received: 24 January 2018

Accepted: 4 March 2018

Published: 5 April 2018


Himachal Pradesh is a northern state of India and is situated to the southern of the mighty Himalaya. The agro-climatic conditions are conducive for the production of off-season vegetables. Biological control by entomopathogenic nematodes (EPNs) is one of the alternatives to manage the turnip moth, Agrotis segetum (Denis & Schiffermuller) (Lepidoptera: Noctuidae). The present study was, therefore, undertaken with the objective to assess the virulence of local EPN isolates from Himachal Pradesh, Heterorhabditis sp., against pests to minimize the use of insecticides during crop protection. Against L3–L5 of A. segetum, Heterorhabditis indica was found highly effective, resulting in 33.33–93.33% mortality at 40 infective juveniles (IJs)/larva after 96 h of treatment. Among local isolates, Heterorhabditis sp. (HSG) influenced maximum mortality of L3 and L4, whereas in L5, H. bacteriophora (HRJ) influenced maximum mortality. The sensitivity of L3 to different EPNs was (7.0–16.6%) higher than that of L4. In soil bioassay carried out against L4 of A. segetum, H. bacteriophora (HRJ) was found highly effective, followed by Heterorhabditis sp. (HKM) and Heterorhabditis sp. (HSG) at 10,000 IJs/kg of soil. The mortality varied from 78.33 to 81.67% with local isolates after 7 days of treatment. The results suggested that EPNs can be used as one of the components for managing A. segetum under field and greenhouse conditions to reduce over dependence on insecticides.


Agrotis segetum Biological controlEntomopathogenic nematodesPotentialVegetable crops


The turnip moths are polyphagous and cosmopolitan pests attacking a large number of crops worldwide (Napiorkowska and Gawowska 2004). The genus Agrotis includes many species of turnip moths which cause extensive damage to several vegetable and field crops in India. Five species of cutworm viz., Agrotis ipsilon (Hufnagel), A. segetum (Denis & Schiffermuller), A. flammatra Denis & Schiffermuller, A. interacta Walker, and A. spinifera Hubner have been reported damaging potato and other crops in India (Chandel et al. 2007). Verma and Verma (2002) reported that A. segetum and A. ipsilon as predominant species attack various crops (3–18% infestation) in Himachal Pradesh, India. A. segetum is a leading species causing extensive damage in vegetables, ornamentals, and field crops. A. ipsilon is prevalent in low and mid hills, whereas A. segetum is more abundant in higher elevations (Anonymous 2003). Lv et al. (2006) and Esbjerg and Sigsgaard (2014) reported that A. segetum is distributed throughout the temperate regions of Europe, Africa, and Asia. Turnip moth larvae which become photonegative by the fourth instar hide in the soil during the daylight hours. In these latter instars, they also tend to severe plants at the soil surface, pulling the plant tissue below ground (Sharma et al. 2012). However, application of soil insecticides is generally ineffective because of their difficulty in translocation through the soil into the root zone. In early stages, the crops suffer severely and irreparably and the crop stand is considerably reduced. This is particularly true for cole crops like cauliflower and cabbage, the important cash crops in north western Himalaya. Farmers used large quantity of farm yard manure (FYM) to increase their yields but the excessive of using FYM enhances the severity of cutworm incidence.

The control of Agrotis spp. through chemicals has been reported by various researchers in India (Chandel and Chandla 2003 and Sharma et al. 2012). Because of the difficulty in managing turnip moth through the use of insecticides, special attention has been focused on entomopathogens, especially nematodes (EPNs). The EPNs have been used as biological control agents to effectively impress a large number of economically important insect pests (Grewal et al. 2005 and Laznik et al. 2010). Hassan et al. (2009) have observed steinernematids and heterorhabditids to infect over 200 species of insects belonging to different orders. EPNs have been applied successfully against soil-inhabiting insects (as soil application) as well as above-ground insects (foliar spray) in cryptic habitats (Arthurs et al. 2004; Trdan et al. 2007; and Laznik et al. 2011). According to Sharma et al. (2011), the performance of EPNs has got more success in controlling soil insects when compared to foliar pests. They are mobile in soil environment and have a potential in inundative and inoculative releases with persistence for years (Bathon 1996). However, the efficacy of most biological control agents including EPNs declines with advancement of larvae into higher instar stages (Laznik and Trdan 2015). Recently, Vashisth et al. (2015) surveyed the EPNs in temperate areas of Himachal Pradesh and identified virulent strains of Heterorhabditis spp.

The present study aimed to evaluate the potential of the species/strains of Heterorhabditis spp. against A. segetum, native to north western Himalaya.

Materials and methods

Soil sampling and nematode extraction

Soil samples were collected during 2011–2013 from different parts of Himachal Pradesh, India. The samples were transported to the laboratory at the Himachal Pradesh Agriculture University (HPAU), Palampur, Himachal Pradesh, India, and kept in a cool place until processing. Information on sampling date, location, soil type, habitat, longitude, latitude, and altitude was recorded for each sample (Vashisth et al. 2015). EPNs were recovered from soil samples, using an insect baiting method described by Bedding and Akhurst (1975). Greater wax moth, Galleria mellonella (Linnaeus), and rice moth, Corcyra cephalonica (Stainton), were used as baiting agents. Twenty last-instar larvae of either of these insects were placed at the bottom of a jar containing 250 g moistened soil each sample and kept at room temperature (25 ± 2 °C) for 1 week. Dead larvae from the container were examined for the presence of nematodes and placed in White traps (White (1927)) to collect the emerging infective juveniles (IJs). To verify the pathogenicity of collected nematodes and to establish new cultures, the emerging nematodes were pooled in each sample and used to infect fresh A. grisella and C. cephalonica larvae. For identification, the nematodes were placed in Ringer’s solution (Kaya and Stock 1997) on an object glass, covered with a ring glass. Adams and Nguyen (2002) methodologies were used for the nematode identification. For further studies, mass production of the nematode population was carried out, using chicken offal medium (Bedding 1984). After about 20 days of inoculation, harvesting of IJs was done following the method of Tabassum and Shahina (2004). The pathogenicity of the harvested IJs was tested three times against the last-instar larvae of A. grisella and C. cephalonica. All the experiments were performed within 15 days of emergence.

Insect culture of turnip moth, Agrotis segetum

The turnip moth culture was maintained according to the procedure standardized by Verma (1996). First-instar larvae were maintained on soft cabbage leaves grown in small plastic containers (5.5 × 7 cm). The petiole end of each leaf was pressed into a piece of wet cotton to maintain the turgidity of the leaf for a longer period. The larvae were cultured en masse up to the third-instar larvae (L3); however, after L3, the larvae showed cannibalism against each other; therefore, subsequent culturing was undertaken in glass jars (15 × 18 cm), filled with mixture of soil + sand up to 10 cm. Fresh cabbage leaves were provided regularly in jars as food for developing larvae, and the leaves were changed frequently. The full-grown larvae pupated in the soil, and the adult moths emerged out in about 15 days. The adults were sexed on the basis of their antennae and were transferred to glass chimneys for mating. A crumpled paper was placed in each chimney to support easy repose of moths on these sites. In each glass chimney, 10% honey solution was kept in a small Petri plate and three pairs of A. segetum were released in it. The eggs were collected by a camel hair brush and placed on the moist filter paper in the Petri plate for hatching.

Evaluation of local EPN isolates against Agrotis segetum

Petri plate bioassay

Efficacy of the three local EPNs (Heterorhabditis sp. (HSG), Heterorhabditis sp. (HKM), and H. bacteriophora (HRJ)), obtained during the survey from Sangla, Kamand, and Rajgarh areas of Himachal Pradesh, India, along with commercially available formulation of Heterorhabditis indica (National Bureau of Agricultural Insect Resources, Bengaluru, India), were tested against L3–L5 of A. segetum, following the Petri plate bioassay. For Petri plate bioassay, a Whatman No. 1 filter paper (9.5 cm in diameter) was placed in a sterile Petri dish (9 cm in diameter). The EPN concentrations were adjusted to 10, 20, 30, and 40 IJs/larva. Healthy and uniform size laboratory-reared larvae of test insects were used for bioassay studies. The larvae were exposed to 1 ml nematode suspension at 10, 20, 30, and 40 IJs/larva on to a moist filter paper lined in a Petri dish (10 larvae per Petri plate), and each set of treatment was replicated three times. In the control, only 1 ml of distilled water was used. The insects were provided with their natural food. Inoculated plates were incubated at 25 ± 1 °C. The insect mortalities were checked, 24, 48, 72, and 96 h post inoculation. Mortality data were corrected using Abbott’s formula (Abbott 1925). The corrected mortality data were analyzed on the pattern of a completely randomized design, using three-way tables.

Soil inoculation bioassay

This method was used only against L4 of A. segetum against all species of EPNs (Fig. 2). For testing, 1 kg sterilized moist soil was placed into a plastic/glass jar. Different concentrations of nematodes (1000, 5000, and 10,000 IJs) were applied in 10 ml distilled water per kilogram of soil. In each jar, 10 turnip moth larvae (L4) were released, and tender cabbage leaves were also placed in the jars as food for the larvae. There were three replications for each treatment. The glass jars were covered with muslin cloth and kept at room temperature. The insect mortalities were checked 3, 5, and 7 days post inoculation. For data recording, the soil was tipped out and the larvae were gently searched in soil. The healthy and dead larvae were counted, and the soil was filled again in the same jars along with the healthy larvae. Mortality data were corrected using Abbott’s formula (Abbott 1925), which were used for further analysis, using a completely randomized design.

Statistical analysis

The insect mortality was corrected using Abbott’s formula (Abbott 1925). Data were subjected to ANOVA using Genstat Version 14.0. Significance of differences between the isolates was tested by F test, while the treatment means were compared by LSD at P ≤ 0.05.

Results and discussion

Petri plate bioassay

There were significant differences in mortality of L3 of A. segetum across EPN isolates. H. indica caused the maximum larval mortality (93.3%) 96 h post treatment, at the concentration of 40 IJs/larva (Fig. 1a). Among the locally extracted EPN isolates, Heterorhabditis spp. (HKM) caused a maximum mortality (81.3%) of A. segetum in comparison to the other two isolates. The data revealed significant differences in the percent larval mortality of A. segetum for each nematode species, population species (S) wise F = 43.2, df = 3, p = < 0.001; exposure (E) period wise F = 421.4, df = 3, p = < 0.001; and population (P) number wise F = 228.52, df = 3, p = < 0.001.
Fig. 1

The percentage mortality of different instar larvae of Agrotis segetum, following exposure to different concentrations of infective juveniles (IJs) of nematodes under laboratory conditions (Petri plate bioassay). a Third instar. b Fourth instar. c Fifth instar

The differences in potential of the different isolates of EPNs against L4 of A. segetum were insignificant but differences across the concentrations were significant. All EPN isolates caused equivalent amount rates of larval mortality 96 h post inoculation, at the concentration of 40 IJs/larva (Fig. 1b). The data revealed significant differences in percent larval mortality of A. segetum for each nematode species, population S wise F = 30.36, df = 3, p = < 0.001; E period wise F = 323.19, df = 3, p = < 0.001; and P number wise F = 183.31, df = 3, p = < 0.001.

Among local EPN isolates, H. bacteriophora (HRJ) caused the maximum larval mortality in L5 of A. segetum 96 h post inoculation, at the concentration of 40 IJs/larva (Fig. 1c), whereas the commercial isolate H. indica caused the maximum larval mortality (56.6%). The data revealed significant differences in larval mortality of A. segetum for each nematode species, population S wise F = 0.7, df = 3, p = < 0.001; E period wise F = 112.78, df = 3, p = < 0.001; and P number wise F = 70.07, df = 3, p = < 0.001. Comparative data pertaining to efficacy of Heterorhabditis sp. (HSG), Heterorhabditis sp. (HKM), H. bacteriophora (HRJ) and H. indica 96 h post inoculation, at the concentration of 40 IJs/larva against L3-L5 of turnip moth were illustrated in Fig. 2.
Fig. 2

Relative susceptibility of Agrotis segetum against different EPN isolates

Soil inoculation bioassay

Heterorhabditis sp. (HSG) achieved 28.33% mortality of A. segetum L4 at 10,000 IJs/kg soil, whereas at 1000 IJs/kg of soil, only 3.33% mortality was recorded 3 days post treatment. After 5 days of treatment, 8.33, 30.10, and 53.33% mortality were recorded at 1000, 5000, and 10,000 IJs/kg soil, respectively. The highest mortality rate (78.33%) was obtained at 10,000 IJs/kg of soil 7 days post treatment (Fig. 3a). The data revealed significant differences in larval mortality rates of A. segetum for each nematode species, population S wise F = 16.67, df = 3, p = < 0.001; E period wise F = 462.68, df = 2, p = < 0.001; and P number wise F = 772.24, df = 2, p = < 0.001.
Fig. 3

The percentage mortality of fourth-instar larvae of Agrotis segetum following exposure to different concentrations of infective juveniles (IJs) of nematodes under laboratory conditions (soil inoculation method). a Heterorhabditis sp.(HSG). b Heterorhabditis sp.(HKM). c H. bacteriophora (HRJ). d H. indica

Heterorhabditis sp. (HKM) showed a slightly higher mortality against L4 of A. segetum, compared to Heterorhabditis sp. (HSG) at the tested concentrations. There was 31.67% mortality with this EPN isolate, at 10,000 IJs/kg of soil 3 days post treatment. After 5 and 7 days of treatment, the concentration of 10,000 IJs/kg soil caused 58.33 and 80.00% mortality rates, respectively. At the population level of 5000 IJs/kg soil, the mortality varied from 18.33 to 58.33%, 3 to 7 days post treatment. The minimum mortality rate (21.67%) was recorded 7 days post treatment, at 1000 IJs/kg of soil (Fig. 3b).

H. bacteriophora (HRJ) was comparatively more effective than Heterorhabditis spp. (HSG) and Heterorhabditis sp. (HKM). There achieved 20.00 and 33.33% mortality rates, at 5000 and 10,000 IJs/kg soil, 3 days post treatment. The lowest concentration of 1000 IJs/kg of soil recorded 3.33–26.67% mortality, 3 to 7 days post treatment. At a concentration of 5000 IJs/kg of soil, the mortality rate varied from 36.67 to 60.00%, 5 to 7 days post treatment. The maximum mortality rate (81.67%) was recorded at 10,000 IJs/kg soil 7 days post treatment (Fig. 3c).

Comparative data pertaining to efficacy of Heterorhabditis sp. (HSG), Heterorhabditis sp. (HKM), and H. bacteriophora (HRJ) were illustrated in Fig. 4. All the three local isolates were statistically at par with each other, at the concentration of 1000 IJs/kg soil. The mortality rate varied from 11.66 to 13.89% at this concentration. At 5000 IJs/kg of soil, H. bacteriophora (HRJ) and Heterorhabditis sp. (HKM) showed 38.89 and 37.22% mortality and both were statistically at par with each other. Heterorhabditis sp. (HSG) recorded 33.33% mortality rate, which differed significantly than Heterorhabditis sp. (HKM) and H. bacteriophora (HRJ). At the highest population level of 10,000 IJs/kg of soil, Heterorhabditis sp. (HKM) was statistically at par with Heterorhabditis sp. (HSG) and H. bacteriophora (HRJ), whereas Heterorhabditis sp. (HSG) differed significantly than H. bacteriophora (HRJ). The highest mortality rate (57.78%) was recorded by H. bacteriophora (HRJ) at this concentration. The mortality rate varied from 16.11–18.89%, 3 days post treatment, and the differences among isolates were insignificant (Fig. 4).
Fig. 4

Relative susceptibility of Agrotis segetum against different EPN isolates

In order to study the reproduction of EPNs, the A. segetum larvae were exposed to 10, 20, 30, and 40 IJs/larva of each nematode species. The host mortality rate and the emerging IJs from host cadavers were collected and counted. The data revealed that all four test nematodes were successfully invaded and propagated in the insect larvae and produced IJs (Figs. 5ad). It was also evident that all nematode species exhibited a linear relationship between the concentrations of IJs applied and the total number of IJs produced per infected larva. In this study, H. indica and H. bacteriophora (HRJ) produced significantly more number of IJs per insect larva than the other two nematode species (Fig. 2c, d). For H. bacteriophora (HRJ) and H. indica, the maximum production of IJs per larva (14.23 ± 1.34 × 103 IJs/larva and 11.09 ± 1.14 × 103 IJs/larva) was obtained at 40 IJs/larva concentration. Among the four EPNs studied, the least progeny production was recorded for Heterorhabditis sp. (HKM). It increased linearly with the increase of IJ concentration where it reached its maximum of 7.62 ± 1.04 × 103 IJs/larva, at the concentration of 40 IJs/larva.
Fig. 5

Production of infective juveniles in Agrotis segetum proxima larvae at different dosages of infective juveniles. a Heterorhabditis sp. (HSG). b Heterorhabditis sp. (HKM). c H. bacteriophora (HRJ). d H. indica. **p < 0.01%; means shown by the same letter are not significantly different (p > 0.05)

There were significant differences in the efficacy of different isolates of EPNs against the L3 of A. segetum across concentrations and the observation periods. The efficiency of EPNs against a given host partly depends on the host-finding, ability, and penetration capability of the IJs (Peters & Ehlers, 1994). EPNs have been tested against a large number of insect pest species, with results varying from poor to excellent control (Laznik and Trdan 2015). Many factors can influence the successful use of nematodes as biological agents, but matching the biology and ecology of both the nematode and the target pest is a crucial step towards successful application. The H. bacteriophora (HRJ) resulted in a significantly greater mortality of A. segetum than the isolates from Sangla and Kamand across the isolates and observation periods, though the differences in larval mortality between the isolates were much smaller. These findings are in partial agreement to the findings of Chandel et al. (2009) who reported 100% mortality of L3 and L4 of A. segetum in a Petri plate bioassay, at 10–40 IJs/cm2, due to infection with H. bacteriophora. Fetoh et al. (2009) tested an Egyptian strain of H. bacteriophora against L4 of A. ipsilon under laboratory conditions and recorded 80 ± 4.0 to 100 ± 0.0% mortality rate, at 25–100 IJs/ml and reported that H. bacteriophora was highly virulent against A. ipsilon. Larval mortality may be presumed to be related to the number of viable nematodes ingested by the insect during feeding, or infection could take place by invasion of the nematodes through the natural openings/cuticle of the insect with an undetermined minimum number required for mortality to occur. The maximum mortality (93.3%) was caused by H. indica (commercially available isolate during studies) against L3 of A. segetum. Hussaini et al. (2005) studied the infectivity of H. indica PDBC EN6.71 along with other EPNs against the last-instar larvae of A. ipsilon and obtained absolute mortality after 48 and 72 h at 25 and 32 °C. Against A. segetum, there was a 66.70% mortality rate, with H. indica PDBC EN 6.71 72 h post inoculation (Hussaini et al. 2000). Yan et al. (2014) also reported that H. indica LN2 was the most virulent and promising species, causing 83.3% mortality to the L3 72 h post infection.

In the soil bioassay carried out against L4 of A. segetum, H. bacteriophora (HRJ) was found highly effective, followed by Heterorhabditis sp. (HKM) and then Heterorhabditis sp. (HSG) at10,000 IJs/kg of soil. The mortality rate varied from 78.33 to 81.67% 7 days post treatment, at local isolates. Chandel et al. (2009) found that the concentration of 1000 IJs/kg of soil of H. bacteriophora was sufficient to initiate infection in the larvae of A. segetum. They reported 61.3–91.6% mortality rate in L4 at 1000–10,000 IJs/kg of soil. Obtained data support the findings of Hussaini et al. (2001) who recorded that Heterorhabditis was virulent against A. ipsilon larvae in sand column assay. Gupta (2003) studied the efficacy of EPNs against A. ipsilon and found that the nematodes applied as foliar spray (50–100 IJs/larva), paper wrapping method (25–75 IJs/larva), and food dip (25–75 IJs/larva) caused 40–80, 100, and 40–60% pest mortality rates, respectively.

When A. segetum were exposed to Heterorhabditis spp. in sand, higher inoculation concentrations were required as compared to inoculation concentrations on filter paper. The differences in the two inoculation methods may be attributed to the differences in the inoculation substrates and bioassay arenas. In the sand bioassay technique, A. segetum was exposed to Heterorhabditis spp. in three-dimensional substrates, where the nematode has to search the insect host. In the Petri plate bioassay method, A. segetum was exposed to Heterorhabditis spp. in two-dimensional substrates, where the nematode and the host were in direct and close contact with each other. Obtained results are in agreement with that of Grewal et al. (1994) who reported that the substrate had a profound effect on host finding by EPNs. A major factor that restricts the EPNs’ host range was the foraging behavior of the IJs. These nematodes employed different foraging strategies to locate and infect hosts that range from one extreme of sit-and-wait (ambush) to the other of widely foraging strategy (cruise) (Lewis 2002; Laznik and Trdan 2013).

Timing of nematode applications is also an important consideration. Different turnip moths may arrive to the root feeding zone near the soil surface at varying times during the growing season. Nematodes applied too early may provide poor insect control and may not reach deep in the soil before their upward seasonal migration. To overcome the dispersal behavior of the EPNs, Heterorhabditis spp., so that it can infect its host, the application of high dosages to the soil surface may increase the infection rate. However, it is also important to note that results from the laboratory tests are not always comparable to field testing (Cantelo and Nickle 1992) as the functioning of EPNs in the open is influenced by an extensive list of factors. In one relevant study, the 100% efficacy rate of S. carpocapsae in controlling Colorado potato beetle adults, pupae, and larvae in the laboratory manifested as only a 31% reduction rate in this pest population when the test was repeated outdoors (Stewart et al. 1998).

Reproducing and recycling of EPNs in a host play an important role in their persistence in the soil and also in their overall effectiveness in pest control (Georgis and Hague 1991). A prior knowledge about reproducing and recycling nematodes is considered important in determining the time and concentration of subsequent EPN application, which may be useful in reducing the cost of application. The data in the present study suggested that following application, all the tested species of nematodes were able to infect and propagate within the insect host and produce IJs. The evidence obtained in this study suggests that all three tested indigenous species of EPNs were virulent enough to produce 100% mortality to the larvae of A. segetum. Furthermore, all EPNs could also propagate in the infected larva and produce F1 generation IJs.


The results showed that H. indica could offer a great potential and a higher virulence than other species/strains of EPNs against the larvae of A. segetum. EPNs (Heterorhabditis sp. (HSG), Heterorhabditis sp. (HKM), and H. bacteriophora (HRJ)) had a potential to be used as biocontrol agents for the integrated management of A. segetum in vegetable crops and flowers under protected cultivations. Among the local isolates, Heterorhabditis sp. (HSG) recorded the maximum mortality rates during L3–L4, and the sensitivity of larva decreased with increasing the larval age. Hence, EPNs prudent to target young larvae as compared to older ones. In soil bioassay, local isolates gave very high mortality rates within a week’s time. The EPNs can propagate in the infected larvae and produce IJs; hence, they hold a good potential to be used as inoculative agents in soil for biological control of turnip moth larvae. The soil provides the most congenial conditions where IJs can survive, persist, establish, recycle, and develop a long-term regulation of turnip moths and other soil pests.



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Authors’ contributions

SV carried out the bioassay studies, statistical analysis and drafted the manuscript. YS participated in the design of the study and assisted in the statistical analysis. RS conceived of the study and participated in its design and coordination. All authors read and approved the final manuscript.

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Authors’ Affiliations

Department of Entomology, CSK Himachal Pradesh Agriculture University, Palampur, India
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India


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