Ultrastructural alterations in the midgut of Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) larvae

Culex pipiens Linnaeus complex mosquitoes transmit several diseases that affect humans and other animals (Michalski et al. 2010). These species oviposit in stagnant polluted water and populations are increasing and expanding due to creation of favorable habitats caused by urbanization (Bockarie et al. 2009), irrigation, and creation of the Aswan High Dam (Harb et al. 1993). Mosquito control strategy relies heavily on insecticides and particularly pyrethroids. However, widespread of pyrethroid resistance has hindered vector control implementation and sustainability (Shi et al. 2015).

Highly potent mosquitocidal strains of the microbial agent Bacillus sphaericus have been applied for the control of mosquito larvae around the world (Mulla et al. 2003). This organism has several advantages, including low environmental toxicity due to the high specificity of B. sphaericus toxins, high levels of efficacy and environmental persistence, and the ability to overcome resistance developed against conventional insecticides used worldwide (Nielsen-LeRoux et al. 2001). B. sphaericus crystal contains two major polypeptides, a 42-kDa polypeptide and a 51-kDa polypeptide, which are designated BinA and BinB, respectively (Darboux et al. 2007). The mode of action of the toxin complex in susceptible mosquitoes involves highly specific binding to a receptor in the larval midgut (Silva-Filha et al. 1997). The two crystal components act synergistically; the BinB part is responsible for initial binding to the receptor, and the BinA component confers toxicity (Charles et al. 1997). A direct correlation exists between binding affinity and toxicity (Opota et al. 2008).

In mosquitoes, the first physical barrier met by ingested pathogens is the midgut epithelium; it is composed of a single layer of epithelial cells forming a microvillar surface on the luminal side (Hecker 1977).

The present study describes the ultrastructure alterations in the midgut of Cx. pipiens larvae following B. sphaericus treatment.

Examination of non-treated mosquito midgut larvae revealed that it was composed of a single-layered epithelium surrounded by a network of circular and longitudinal muscle bundles (Fig. 1a, b). The digestive cells had morphological characteristics of enterocyte cells and basolateral membrane. The free surface of enterocytes had a regular array of microvilli. Cells have spherical nuclei. Results of the present study were comparable to those found by Wassim et al. (2014) working on Cx. pipiens midgut epithelium.

The morphological structures of B. sphaericus-treated Cx. pipiens larval midgut showed modifications at the various timing following B. shaericus treatment. Two hours post-treatment, the epithelial layer began to have large amount of vacuoles (Fig. 2a). The microvilli were not affected (Fig. 2b). Examination revealed the presence of vacuoles in lumen cytoplasm. The early appearance of cytological disturbances, following bacterial treatment, could be correlated to the fast digestion of B. sphaericus cells in the anterior and central midgut (Labib and Dawoud 2003). Cytoplasmic vacuolization was reported to be one of the major cytotoxic responses of Cx. mosquitoes to Bin intoxication (Silva-Filha et al. 1997). B. sphaericus bin-induced vacuolization was a transient phenomenon that affects autolysosomes and vacuolization was associated with induction of autophagy in intoxicated cells (Opota et al. 2008). Large vacuoles appeared early in the Cx. pipiens mid-gut cells and rough endoplasmic reticula broke into small vesicles.

Six hours post-treatment, the amount of vacuoles increased in the epithelial layer (Fig. 3a, b). This timing showed the onset of abnormalities in the nucleus. Although microvilli were not affected, some positions lost their microvilli. Explosion in the basement membrane made it losing contact, a result that could be explained by the fact that Cx. pipiens larval midgut was the primary target of the binary toxin (Bin) present in parasporal inclusions of B. sphaericus (Darboux et al. 2001).

Ten hours post-treatment, B. sphaericus spores were seen in the lumen (Fig. 4a). Microvilli were still intact (Fig. 4b). However, the bacterium began to invade the microvilli. At this time, the presence of bacteria was evident in the lumen. Bacteria began to enter the epithelial layer. The midgut epithelial cells of mosquito larvae intoxicated with Bin was reported to display several cytopathologies affecting the microvilli, the mitochondria, and the rough endoplasmic reticulum, but the most dramatic feature of Bin intoxication was the appearance of abnormal, electron-clear vacuoles indicating an important cellular stress (Silva-Filha et al. 1997). Bin toxin triggered apoptosis via an intrinsic or mitochondrial pathway in vivo, possibly contributing to larval death (Tangsongcharoen et al. 2015). However, proliferation and differentiation of precursor stem cells could replace the destroyed midgut epithelial cells (Baton and Ranford-Cartwright 2007).

At 14 h post-bacterial treatment, bacteria were present in lumen in large amount (Fig. 5a), the result that agreed with that of Labib and Mohamad (2003) who found that the number of viable spores reached its maximum between 12 and 24 h. Epithelial cells were destroyed. Microvilli were damaged as well (Fig. 5b). Binding to specific receptors on the apical microvilli membrane was considered as the initial step of delta-endotoxin action (Ravoahangimalala et al. 1993). Similarly, Cry4 produced by B. thuriengiensis israelensis were proved to bind to the microvilli of the epithelial cells of Cx. pipiens posterior midgut and gastric caecae (Yamagiwa et al. 2001). On the contrary, microvilli of the midgut epithelial cells of Streptomyces griseus-treated Culex pipiens autogenicus larvae were unaltered, and there were no changes in the arrangement of cells in the tissues (Zizka et al. 1989).

Structural alterations in larval midgut following bacterial treatment are evident and enhance as the time proceeds. The present study provided a description of the main aberrations induced in midgut larvae of Cx. pipiens as a result of B. sphaericus treatment.

The present study provided the evidence on the main aberrations induced in midgut larvae of Cx. pipiens as a result of ingesting B. sphaericus.