Biosynthesis and characterization of silver nanoparticles using Trichoderma longibrachiatum and their effect on phytopathogenic fungi
© The Author(s) 2018
Received: 27 September 2017
Accepted: 8 January 2018
Published: 8 March 2018
An efficient biosynthesis process for the rapid production of nanoparticles would enable the development of a “microbial nanotechnology” for mass-scale production. In the present research, biological silver nanoparticle was synthesized extracellularly by using the fungus, Trichoderma longibrachiatum, where the cell filtrate of the fungus was used as a reducing and stabilizing agent in the process of nanoparticle synthesis. Different physical parameters such as fungal biomass concentration (1, 5, 10, 15, and 20 g), temperature (25, 28, and 33 °C), incubation time (0–120 h), and agitation (shaken or not shaken) were investigated, in order to determine the optimal conditions for nanoparticle biosynthesis. The stability and antifungal properties of the synthesized silver nanoparticles (AgNPs) were also determined. Data revealed that a combination of 10 g fungal biomass, a reaction temperature of 28 °C, a 72-h incubation time, and without shaking were the optimum conditions for the synthesis of the silver nanoparticles. Visual observation of brown color is an indication of silver nanoparticle production. UV–vis spectroscopy showed maximum absorption at 385 nm with the optimum conditions. Transmission electron microscopy (TEM) revealed the formation of monodispersed spherical shape with a mean diameter of 10 nm. Fourier transformation infrared (FTIR) showed bands at1634.92 and 3269.31 cm−1. Dynamic light scattering (DLS) supported that the Z-average size was 24.43 and 0.420 PdI value. Zeta potential showed − 19.7 mV with a single peak. The AgNPs synthesized through this biosystem approach were relatively stable up to 2 months after synthesis. The use of AgNPs as antifungal led to significant reductions in the number of forming colonies for many plant pathogenic fungi, with efficiencies reaching up to 90% against Fusarium verticillioides, Fusarium moniliforme, Penicillium brevicompactum, Helminthosporium oryzae, and Pyricularia grisea. However, further research should be carried out in order to determine the toxic effect of AgNPs before mass production and use of agricultural applications.
Silver nanoparticles are widely used in many applications in various fields such as bio-labeling, sensors, antimicrobial agents, filters, microelectronics, and catalysis. This is because of their specific physico-chemical and biological properties (Pal et al. 2007; Ingle et al. 2008; Kim et al. 2008; Rai et al. 2009). Silver nanoparticles (AgNPs) have inhibitory effects on the growth of bacteria, virus, and other eukaryotic microorganisms (Jeong et al. 2005). In addition to their distinctive properties, their production cost is relatively low (Capek 2004). Physical and chemical methods have been utilized for the synthesis of nanoparticles (Mandal et al. 2005; Solanki and Murthy 2010; Huang et al. 2011). Basically, the physical methods have affected low yields, while the chemical ones caused harmful effects on the environment due to use of toxic solvents and the regeneration of hazardous by-products (Wang et al. 2007). Biosynthetic methods have been investigated as an alternative to chemical and physical methods. Microorganisms have been explored as potential bio-factories for synthesis of metallic nanoparticles such as cadmium sulphide, gold, and silver (Sastry et al. 2003). Biosynthesized nanoparticles are eco-friendly, reliable, biocompatible, and low cost (Roy et al. 2013; Emeka et al. 2014).
Silver nanoparticles may be less toxic to humans and animals than synthetic fungicides. Moreover, the toxicity that nanoparticles may cause in algae, plants, and fungi may be coupled with some positive effects (Sondi and Salopek-Sondi 2004). Recent studies in mammals and human cells have demonstrated that AgNPs are cytotoxic to various types of cells (Vandebriel et al. 2014; AshaRani et al. 2009). Other studies have shown that AgNPs accumulate in various types of cells (blood, liver, lungs, kidneys, stomach, testes, and brain) but showed no significant genotoxicity function (Kim et al. 2008). AgNPs effectively inhibit microorganisms without causing significant cytotoxicity also; AgNPs are nontoxic in mice and guinea pigs when administered by oral, ocular, and dermal routes. These conflicting results reveal the difficulty of pinpointing the overall toxicity of AgNPs to humans because the tremendous variation in particle size, particle aggregation, and concentration or coating thickness (in implants) of AgNPs has different profiles of silver release and bioactivity. In short, much longer and detailed studies have to be carried out to seriously consider AgNPs’ potential toxicity in humans (Sharma et al. 2009; Murphy et al. 2015).
Biologically synthesized AgNPs could have many applications (Schultz et al. 2000; Durán et al. 2005). Different genera of fungi have been reported to synthesize metal nanoparticles (Ahmad et al. 2003; Mukherjee et al. 2002; Sastry et al. 2003; Mandal et al. 2005).
The fungal genus Trichoderma (Ascomycetes, Hyprocreales) contains species that are of vast economic importance owing to their production of industrial enzymes and antibiotics (Sivasithamparam and Ghisalberti 1998) and their ability to act as biological control agents against plant pathogens. In the biosynthesis of metal nanoparticles by a fungus, its mycelium is exposed to the metal salt solution, which prompts the fungus to produce enzymes and metabolites for its own survival. In this process, the metal ions are reduced to the metallic solid nanoparticles through the catalytic effect of the extracellular enzyme and metabolites of the fungus (Das and Marsili 2010; Vahabi et al. 2011).
The objectives of the present work were to determine the optimal conditions for the synthesis of biogenic silver nanoparticles, using a filtrate of the fungus T. longibrachiatum and its characterization, and also the stability and antimicrobial activity of the synthesized AgNPs against phytopathogenic fungi.
Materials and methods
Isolation and identification of fungal isolates
Fungal isolates were collected from cucumber, tomato, and pepper plants from around Alryadah city, Saudi Arabia, during 2013 and purified, using hyphal tip standard techniques, and maintained on a potato dextrose agar (PDA) slant and stored at 4 °C until further use. Fungal isolates were identified as T. longibrachiatum (cucumber), T. viride (cucumber), T. harzianum (tomato), F. verticillioides (pepper), Alternaria alternate (tomato), and Rhizopus stolonifer (pepper) based on the publication by Barnett and Hunter (1995). Further identification was done by Nano Technology Center for Scientific Services in Egypt, using fingerprint biotechnology according to Harman et al. (2004) and Harman (2006). Three Trichoderma species viz. T. longibrachiatum, T. viride, and T. harzianum were screened for their ability to synthesize silver nanoparticles. The isolates were cultured in PDA and incubated at 25 °C for 5 days and used for extracellular syntheses of silver nanoparticles. The fungal isolates; F. moniliforme, A. flavus, P. grisea, and H. oryzae were obtained from Rice Pathology Department, Sakha, Kafr Elshiekh.
Fungal biomass preparation for AgNP biosynthesis
Optimization conditions for biosynthesis of silver nanoparticles
The effect of fungal biomass (5, 1, 10, 15, and 20 g), temperature (23, 28, and 33 °C), and agitation on constant shaking at150 rpm or without agitation were studied by varying one parameter at a time, keeping the other experimental conditions the same.
Biosynthesis of silver nanoparticles
Experiments were conducted with the fungal biomass and with a liquid medium in parallel. The fungal biomass was brought into contact with 100 ml Milli-Q deionized water for 48 h at 28 °C at150 rpm. After filtration by Whatman filter paper no.1, 50 ml of 1 mM AgNO3 solution was mixed with 50 ml of cell filtrate in a 250 ml Erlenmeyer flask, kept on a shaker at 150 rpm or without shaking at 23, 28, and 33 °C in the dark. The positive control is the only fungal biomass filtrate without the silver nitrate solution, and the negative control is the silver nitrate solution without cell-free filtrate. The reduction of metal ions was routinely monitored by visual inspection of the solution (color change from yellow to brown) and by measuring the ultraviolet-visible spectrophotometer of the solution by periodic sampling of aliquots (1 ml) of the aqueous component. The formed silver nanoparticles were subjected to different instrumental analytical techniques for characterization.
Characterization of silver nanoparticles
Ultraviolet-visible spectrophotometer analysis
The formation of the reduced silver nanoparticles in colloidal solution was monitored by using a UV–vis spectrophotometer (Lambda 25, PerkinElmer, precisely, UK). The absorption spectra of the supernatants were taken between 300 and 700 nm, using a UV–vis spectrophotometer. The deionized water was used as the blank.
Fourier transform infrared (FTIR) spectroscopy
The FTIR spectrum of biosynthesized silver product was recorded on a FTIR instrument mode Nicolet 6700 spectrometer at a resolution of 4 cm−1 attachment. All measurements were carried out in the range of 400–4000 cm−1 at a resolution of 4 cm−1.
Transmission electron microscopy (TEM)
TEM was performed on JEOL, Japan (JEM-1010) instrument, within accelerating voltage of 80 kV after drying of a drop of aqueous AgNPs on the carbon coated copper. TEM grid samples were dried and kept under vacuum in desiccators before loading on a specimen holder. The particle size distribution of AgNPs was evaluated using ImageJ 1.45 s software1493.
Dynamic light scattering
Zetasizer Nano ZS, Malvern Instruments Ltd., UK, was used to determine the size distribution of particles by measuring dynamic fluctuations of light scattering intensity caused by the Brownian motion of the particles. The measurement gave the average hydrodynamic diameter of the particles, the peak values in the hydrodynamic diameter distribution, and the polydispersity index (PdI) that described the width of particle size distribution. The PdI scale ranges from 0 to 1 (with 0 being monodisperse and 1 being polydisperse). All measurements were carried out in triplicate with a temperature equilibration time of 1 min at 25 °C. The data processing mode was set to high multi-modal resolution. The suspension of the synthesized silver nanoparticles was diluted (20, 40, 60, 80, and 100%), and the diluted sample was allowed to filter through a 0.22-μm syringe driven filter unit or without filtration.
Antifungal activity for AgNPs synthesized in in vitro assays
The antifungal activity of nanoparticles was examined on the basis of colony formation technique by in vitro Petri dish (Elamawi and El-Shafey 2013). The antifungal activity of the synthesized AgNPs was examined against the pathogenic fungi: A. alternate, F. oxysporum, F. verticillioides, F. moniliforme, Aspergillus flavus, A. heteromorphus, P. glabrum, P. brevicompactum, P. grisea, and H. oryzae. Fungal spores at a concentration of 105 spores/ml were incubated at 0.5 mM AgNP solution, 1 mM AgNO3 solution, and sterile, distilled water as a control at 28 °C for 24 h in a shaker (120 rpm). A 25-μl aliquot of the spore suspension was spread on PDA. Three PDA plates per combination were used as replicates. The number of formed colonies was counted after 24 and 48 h. The average numbers of colonies from silver-treated spore suspensions were compared to the number of colonies of AgNO3 solution and with the control (water treatment).
The null hypothesis may be rejected, and there is a 95% confidence level that the parameters are not the same as were tested, using one-way analysis of variance (ANOVA), followed by LSD post hoc test, when significant differences were found (P ≤ 0.05). Statistical significance of results obtained from treating fungi with both Ag-NPs and AgNO3 were compared to control.
Results and discussion
Characterization of AgNPs
Three Trichoderma species viz. T. longibrachiatum, T. viride, and T. harzianum were used for biosynthesis of stable AgNPs. Filtrates of each fungal isolates were incubated with AgNO3 and maintained with agitation under dark conditions at 28 °C. Color change from pale yellow to brown appeared after the incubation period of 72 h with the T. harzianum and T. viride, but there was no color change with T. longibrachiatum (Fig. 1b). The appearance of the brown color was an indication of the formation of AgNPs in the medium. The positive control (without the AgNO3 solution, only fungal biomass) and the negative control (pure AgNO3 solution without cell-free filtrate) did not show any change of color for the mixture reaction (Fig. 1b). The above conditions were recommended for the production of silver nanoparticles from T. harzianum and T. viride. However, it could not be recommended to produce silver nanoparticles from T. longibrachiatum. So, optimization of some factors affecting silver production from T. longibrachiatum is required.
UV–visible spectrum of the biosynthesis of silver nanoparticles using the fungi of T. harzianum and T. viride showed a peak at 420 and 420 nm corresponding to the plasmon absorbance of silver nanoparticles for the tested fungi T. harzianum and T. viride, respectively. Particle size analysis using TEM of AgNPsof T. harzianum and T. viride showed a formation of polydispersed AgNPs in a range of 30–50 nm with T. harzianum and 5–40 nm with T. viridi, as reported by Fayaz et al. (2010) and Singh and Balaji (2011).
Optimization conditions for biosynthesis of silver nanoparticles
Effect of quantity of fungal biomass and agitation
Effect of temperature
Three temperatures 23, 28, and 33 °C with five quantities of fungal biomass, without agitation, in the presence of 1 mM AgNo3 were tested. The temperature of 28 °C was favorable for the production of AgNPs by T. longibrachiatum where color changed after 72 h of incubation with AgNO3. The UV–visible absorption spectroscopy of the T. longibrachiatum incubated at 28 °C showed a maximum absorbance at 385 nm. Incubation at 23 and 33 °C showed no change in color when incubated under the same conditions. Reaction temperature had an important effecton enzyme activity. Fayaz et al. (2009) found that the size of biosynthesized silver nanoparticles by T. viride ranged from 10 to 40 nm at 27 °C. While, at a lower temperature (10 °C), the size of silver nanoparticles ranged from 80 to 100 nm. Thus, these findings are different from those which showed that an increase in reaction temperature resulted in a decrease in particle size, whereas a decrease in temperature resulted in an increase in particle size. This could be explained that 28 °C was favorable for biosynthesis of AgNPs by T. longibrachiatum, while 23 and 33 °C were unfavorable.
There is a relation between the particle size and the plasmon peak, as the particles became larger when the plasmon peak shifts to longer wavelengths. Values for Ag particle size and plasmon maxima that were reported with Solomon et al. (2007) indicated that UV spectra were in lower wavelengths (384–414), with 10- to 14-nm particle size. Nair and Pradeep (2003) revealed that UV spectra at 438 nm wavelength gave particle sizes of 60–80 nm. Obtained results indicated that lower wavelengths of UV spectra at 383 nm recorded particle sizes at 1–25 nm at 28 °C.
Effect of filtration
Parametric optimization studies revealed that fungal biomass of 10 or 15 g at 28 °C, without shaking or filtration, is favorable for the production of AgNPs by T. longibrachiatum. There was always a continuous interaction between the organisms and the environment they lived in.
FTIR spectroscopy analysis
Transmission electron microscope
Particle size analysis of AgNPs by dynamic light scattering (DLS) system
Stabilization of AgNP synthesis by T. longibrachiatum against agglomeration
The stability of the synthesized particles was monitored for up to 60 days by using UV–vis spectral analysis, zeta potential. The UV–visible spectra of AgNPs showed an absorption peak at a wavelength of 414 nm. Moreover, the Z-average size of AgNPs was 90.27 nm PdI value and − 0.8 mV zeta potential. FTIR was used to confirm the stability of AgNPs, where it revealed two distinct peaks at 1635.34 and 3269.31 cm−1. Similar results were obtained as mentioned earlier (Fig. 8). The stability of nanoparticles in solution is very important with respect to antimicrobial activity as unstable AgNPs will not be able to disperse homogeneously, thus reducing the efficacy.
Antifungal activity of biosynthesized AgNPs
Inhibitory effect of synthesized AgNPs by Trichoderma longibrachiatum for different fungi
AgNO3 efficiency (%)
AgNP efficiency (%)
P value < 0.05
Durán et al. (2005) attributed the antimicrobial activity of AgNPs to the formation of insoluble compounds by inactivation of sulfhydryl groups in the fungal cell wall and disruption of membrane-bound enzymes and lipids resulting in lysis of cell. It was also reported that the process may involve the binding of AgNPs to external proteins to create pores, interfering with DNA replication or forming reactive oxygen species (ROS) such as hydrogen peroxide, superoxide anions, and hydroxyl radicals (Jung et al. 2008; Duran et al. 2016; Ottoni et al. 2017).
In conclusions T. longibrachaitum was used in the biosynthesis of silver nanoparticles. Such type of synthesis may be considered as eco-friendly as it is free from any toxic chemicals or organic solvents during the biosynthesis process. Optimization of various parameters was carried out, using a “one at a time” strategy keeping all other variables constant and varying only one. Incubation at 28 °C, fungal biomass at 10 g, without agitation, and incubation for 72 h resulted in biosynthesis of AgNPs by T. longibrachaitum. Dilution at 20% and filtration, using Millipore filter paper 0.22 μm, were favorable for measuring the particle size distribution, using DLS system. T. longibrachiatum showed a potential for extracellular and stable biosynthesis of silver nanoparticles, as confirmed by UV–vis spectroscopy, which showed an absorption peak at 385 nm. TEM revealed the formation of spherical nanoparticles with size ranging between 5 and 25 nm. FTIR showed the bands at 1634.92 and 3269.31 cm−1. DLS supported that the Z-average (r.nm) was 24.43 nm and 0.420 PdI value. The zeta potential was − 19.7 mV with a single peak.
Further studies are still needed to understand the precise molecular mechanism leading to the formation of AgNPs by biological methods in order to have a better control over the size and polydispersity of these nanoparticles. Further research should focus on the development of silver compounds and mixing with fungicides. At the same time, the environmental tracking of silver when applied in the field is important to assess its impact on environment and human health. This information is imperative for future registration and labeling of the AgNPs if they are to be used as fungicides for crop protection.
This research project was supported by a grant from the “Research Center for Female Scientific and Medical Colleges,” Deanship of Scientific Research, King Saud University.
All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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