In vitro inhibition mechanism of Trichoderma asperellum isolates from corn against Rhizoctonia solani causing banded leaf and sheath blight disease and its role in improving the growth of corn seedlings

One of the primary corn diseases, banded leaf and sheath blight, is carried on by the pathogenic fungus Rhizoctonia solani. Efforts to control R. solani are more directed at environmentally friendly control using a biological control agent, such as Trichoderma spp. as antagonistic agents and plant growth promoter. This study aimed to identify T. asperellum isolates based on molecular characteristics and to determine the in vitro inhibition mechanism against R. solani and its role in enhancing the growth of corn seedlings. The HMRP7, HMRF7A, HMEDF1B, HMEDF6A, and CHM01 isolates were identified as T. asperellum with a genetic distance coefficient value of 0.000 and a very high similarity of 100%. Meanwhile, the RsHM isolate was identified as R. solani with a homology level of > 90% and genetic distance coefficient values ranging from 0.000 to 0.032. The antagonistic ability showed that the five T. asperellum isolates were able to inhibit the growth of R. solani in vitro on PDA medium with an inhibition percentage of ≥ 50%, so they were categorized as antagonist agents. T. asperellum showed the antagonistic mechanism in inhibiting the R. solani growth through the action of parasitism. The five T. asperellum isolates tested on corn seedlings showed a significantly high difference from the control treatment on the observational variables of maximum growth potential (MGP), growth rate (GtR), growth simultaneity (GS), vigor index (VI), germination rate (GR), and median germination time (T50). CHM01 isolate showed better potential than other isolates in inhibiting the growth of R. solani in vitro on PDA medium with a parasitism mechanism and enhancing the growth of corn seedlings.


Background
In the past few years, efforts to increase corn production as food, feed, and industry have received serious attention from both farmers and product users.In actuality, numerous plant diseases are extensively present during corn cultivation in the field, which can decrease corn yields' quantity and quality.One of the major corn diseases, banded leaf and sheath blight, is brought on by the pathogenic fungus Rhizoctonia solani.This disease is commonly found attacking corn during the rainy season and reduces the production of corn in the field (Rotasouw et al. 2020).High-value economic crops, particularly corn, are susceptible to this disease causing high-yield losses (Dai et al. 2017).The epidemics of this disease have been reported in recent years in many countries, i.e. in Southeast Asia (Malaysia, Myanmar, Philippines, Thailand, Vietnam, Indonesia); South Asia (India, Sri Lanka, Nepal, Pakistan, Bhutan); East Asia (Japan, Korea, South China); West Africa (Sierra Leone, Ivory Coast, Nigeria); Europe (United Kingdom), South America (Venezuela); and United States (Arkansas) (Zhou et al. 2016).
Having a diverse host range, R. solani is a necrotrophic fungal disease (Adams 1988).This fungus is soil borne, causes different symptoms at each plant growth phase, including root, stem, and crown rots, damping-off, as well as foliar and sheath rot (Baker 1970).This pathogen can endure in soil under suitable environmental conditions for many years by forming sclerotia and mycelia on residual plant debries.In addition, R. solani is a complex fungus with various genetic groups (Matthew and Brooker 1991).These groups are known as anastomotic groups (AGs).Hyphae of the same AG expand their genetic diversity by fusing or anastomosing with each other (Webster and Weber 2007).Because the AG-BI group has been included in into AG-2, R. solani has been assigned to 13 AG, which consists of AG-1 to AG-13 (Carling et al. 2002).According to pathogenicity, morphology, host specificity, nutritional requirements, optimal temperature, and frequency of hyphal anastomosis, single anastomotic groups can be classified into subgroups (Sneh et al. 1991).
Several methods have been taken to overcome plant disease problems, such as the use of fungicides and breeding to produce disease-resistant plants.The use of synthetic pesticides can cause pollution to the environment, and kill non-target microorganisms, besides that being harmful to humans.However, the necessity to use biological control agents as an alternative to control plant pathogens has arisen due to rising organic food demand and public knowledge of the risks of synthetic fungicides (Suryadi et al. 2015).
One biological control agent, Trichoderma sp., is well known for antagonizing soil-borne pathogens both in vitro and in vivo (Mirsam et al. 2022).One of the Trichoderma species that has been widely reported to be able to control soil-borne pathogens is T. asperellum.This fungus was reported to be capable of inhibiting the growth of phytopathogenic fungi, including R. solani, Fusarium verticillioides, F. oxysporum, F. solani, F. camptocerus, F. camptocerus, Alternaria alternata, Botrytis cinerea, Colletotrichum gloeosporioides, Cytospora chrysosperma, and Ganoderma applanatum (Mirsam et al. 2022).Aside from being a biological control agent, Trichoderma sp. has also been widely reported to induce plant growth and development.Trichoderma sp. can indirectly and directly stimulate plant growth by enhancing germination rate and vigor, plant dry weight, promoting flowering, and enhancing plant tolerance to biotic and abiotic stresses, Trichoderma sp. can indirectly and directly stimulate plant growth (Stewart and Hill 2014).Trichoderma sp.stimulates plant growth by increasing the synthesis of phytohormones from both Trichoderma sp. and plants, promoting root development, generating vitamins, speeding up the metabolism of carbohydrates and photosynthesis, and enhancing nutrient absorption and translocation (Harman 2011).Auxin is a phytohormone produced by certain Trichoderma (Contreras-Cornejo et al. 2009), gibberellic acid (GA3), ethylene (Stewart & Hill 2014), and the ability to change the balance of auxins and cytokinins (Martínez-Medina et al. 2014).Therefore, efforts to control R. solani are more directed at environmentally friendly control by using biological control agents, namely Trichoderma sp., which is antagonistic and plant growth promoter.This study aimed to identify T. asperellum isolates based on molecular characteristics and to determine the in vitro inhibition mechanism against R. solani and its role in enhancing the growth of corn seedlings.

Isolation of Rhizoctonia sp. from corn
Rhizoctonia sp. was isolated from corn plants with banded leaf and sheath blight symptoms obtained from farmers' corn plantations in Maros Regency, South Sulawesi, Indonesia (4°13′-5°60′S and 119°42′-120°30′E).Symptomatic samples were cut to a size of 2-3 mm, surface sterilized for 1 min with 1.5% NaClO, and then rinsed three times with sterile distilled water.Next, the parts of the sample that exhibited symptoms were airdried using sterile filter paper.These dried pieces were then placed on potato dextrose agar (PDA) medium and left to incubate for a period of 2-3 days at room temperature between 20 and 25 °C.Furthermore, the fungal hyphae identified as Rhizoctonia sp. were designated as RsHM isolates and propagated on new PDA medium for further examination (Wang et al. 2015).

A stock preparation of Trichoderma sp. isolates
The isolates used in this study were five Trichoderma spp.isolates originating from various ecological niches of corn, namely HMRP7 isolate from rhizoplane, HMRF7A and CHM01 isolates from rhizosphere, HMEDF6A isolate from root endophytic, and HMEDF1B isolate from stem endophytic.The five isolates were obtained from the Research Center for Food Crops, National Research and Innovation Agency, Indonesia.These isolates were then cultured on PDA medium and subjected to incubation at a temperature of 28 °C.Furthermore, stock isolates for testing were prepared by re-cultured on slanting PDA medium, then incubated at 28 °C for 7 days in an incubator.Once the fungi had colonized the slanted medium, they were transferred to a refrigerator set at 4 °C and kept as a stock culture for future use.

Identification of Trichoderma sp. and Rhizoctonia sp. isolates based on morphological characters
The morphological characters were identified on pure Trichoderma sp. and Rhizoctonia sp.isolates.The identification of Trichoderma sp.isolates was based on their morphological characteristics, such as colony color, size, and shape, as well as the size and shape of their phialides and conidia, pigmentation, and growth rate (Matas-Baca et al. 2022).Meanwhile, Rhizoctonia sp.isolate was examined based on its colony characteristics (color, size, shape), the color and shape of its sclerotia, number and size of sclerotia, the color and structure of its hyphae, and its growth rate (Mishra et al. 2014).

Identification of Trichoderma sp. and Rhizoctonia sp. isolates based on molecular characters Preparation of Trichoderma sp. and Rhizoctonia sp. pellets
Trichoderma sp. and Rhizoctonia sp.isolates from the stock were re-cultured on PDA medium.The 7-day-old Rhizoctonia sp. and Trichoderma sp.isolates were suspended using sterile distilled water.To each Petri dish containing Trichoderma sp. and Rhizoctonia sp.isolates, 100 ml of sterile distilled water was added.Next, a spatula was used to collect the suspensions of conidia and mycelium.The conidia and mycelium suspensions were placed in collection bottles.Trichoderma sp. and Rhizoctonia sp.suspensions were each placed into a 1.5 ml microtube and centrifuged for 5 min at 10,000 rpm.After centrifugation, the supernatant was discarded and the resulting pellet was utilized for DNA extraction.

Extraction of Trichoderma sp. and Rhizoctonia sp. DNA
DNA extraction followed the method Mirsam et al. (2022) carried out by using the Zymo Research Quick-DNA Fungal/Bacterial Miniprep Kit.The working principle of this Extraction Kit was that the sample was lysed using bead beating without proteinase.The result of extraction using this kit was Ultra-pure DNA.

Amplification of Trichoderma sp. and Rhizoctonia sp. DNA
The PCR method was used to amplify the extracted DNA.The PCR reaction involved combining 12.5 µl of KAPA Taq ReadyMix PCR, 8.5 µl of ddH2O, 1 µl of forward primer, 1 µl of reverse primer, and 2 µl of DNA template.The primer used was a universal fungus primer, ITS-1 (5'-TCC GTA GGT GAA CCT GCG G-3') and ITS-4(5'TCC TCC GCTTA TTG ATA TGC-3').The PCR cycle consisted of a pre-denaturation step at 95 °C for 3 min, followed by 35 cycles of denaturation at 95 °C for 15 s, annealing at 5 °C for 30 s, extension at 72 °C for 1 min, and final extension at 72 °C for 3 min.A total of 4 µl of indigenous microbial DNA applicon was loaded into the well of a 0.8% agarose gel and electrophoresed at 110 V for 50 min.The electrophoresis results were visualized using a UV transilluminator, and photos were taken with a camera.

Nucleotide sequence analysis
The amplicons obtained were sent to FirstBase services in Malaysia for sequencing.The nucleotide sequences obtained were then analyzed using Bioedit Sequence Alignment Editor 7.2 software with the ClustalW multiple alignment tool for contig analysis of both forward and reverse DNA sequences.The consensus sequences obtained from the contig analysis were subjected to basic local alignment search tool (BLASTN 2.13.0 +) analysis to identify the DNA sequence information available in the National Center for Biotechnology Information (NCBI) database.A phylogenetic analysis between the isolates was carried out using the Molecular Evolutionary Genetic Analysis Software version 11 (MEGA11), with 1000 times replications using the bootstrap method.

Antagonistic ability test of Trichoderma sp. isolate based on the dual-culture method
The antagonistic ability of Trichoderma sp.against Rhizoctonia sp. was carried out using the dual-culture method.The 7-day-old Trichoderma sp. and Rhizoctonia sp.isolates were taken using a cork borer with a diameter of 5 mm and placed in the same Petri dish.The control ( − ) was carried out by preparing PDA medium mixed with a synthetic fungicide with active chemical ingredients of dimethomorph and pyraclostrobin with a concentration of 10%.The PDA medium containing synthetic fungicide was then taken with a diameter of 5 mm using a cork borer and placed in the same Petri dish as Rhizoctonia sp.Meanwhile, for the control ( +), only using Rhizoctonia sp.Each fungus was arranged facing each other at a distance of 3 cm.The fungus was then incubated for 7 days at room temperature.Observations of the radial growth of fungal colonies were carried out every 24 h.until the growth of fungi in control ( +) filled the Petri dish.The test was arranged using a completely randomized design consisting of 7 treatments, namely 5 Trichoderma sp.isolates, 1 synthetic fungicide as control (-), and 1 distilled water as control ( +).The test was repeated 3 times.Percentage growth inhibition (PGI) was calculated using the equation below (Eq.1).
where R1 is the R. solani radius growing in the opposite direction to the antagonistic microorganism, and R2 is the R. solani radius growing towards the antagonistic microorganism (Seema 2012).

In-planta potential test of Trichoderma sp. isolate as plant growth promoter on corn seedling in greenhouse
The in-planta potential test of Trichoderma sp.isolates as an inducer of corn growth was carried out in a greenhouse using seedling trays and followed the method Mirsam et al. (2021).Trichoderma sp.isolates were first re-cultured on PDA medium and incubated at room temperature for 7 days.The seeds of the Anoman variety were first sorted based on their physical characteristics (shape, size, color, and no mechanical damage).The seeds were sterilized by hot water treatment at 60 °C for 2 min, then dried on sterile filter paper in laminar airflow for 20 min.Trichoderma sp.isolates were suspended using sterile distilled water at a conidia concentration of 10 8 cfu/ml.The corn seeds were soaked with Trichoderma sp.suspension in Erlenmeyer and covered with sterile cotton and aluminum foil, and then incubated using a rotary shaker at 180 rpm for 24 h.After soaking, the seeds were air-dried on sterile filter paper in laminar airflow.Furthermore, the seeds were planted in a seedling tray with sterile soil and combined medium (1:1), and then the seeds that have been planted in the tray were arranged in a greenhouse.This test was arranged using a completely randomized design consisting of 6 treatments, namely 5 Trichoderma sp.isolates and 1 distilled water as a control.The test was repeated 3 times.Observation was made 7 days after planting (DAP).The observed variables are seedling height, root length, root wet weight, root dry weight, shoot wet weight, and shoot dry weight.The dry weight was calculated by weighing the plant samples which had been dried in an oven at 60 °C for 48 h.Other observational parameters were maximum growth potential (MGP), growth rate (GtR), growth simultaneity (GS), vigor index (VI), germination (1) rate (GR), and median germination time (T50) based on International Seed Testing Association (ISTA 2023).

Maximum growth potential (MGP)
MGP is the percentage of seeds grown, calculated on the 7th day of observation using Eq. ( 2):

Growth rate (GtR)
The GtR was observed daily on normal seedlings and calculated using Eq. ( 3).
where n = percentage of seedlings that are considered normal for each observation period (%); D = duration of each observation period per 24 h (etmal).

Growth simultaneity (GS)
Observation of GS was carried out on the third and sixth days after the seeds were initially planted.The formula used is (Eq.4): where NS is normal seedling during 1st and 2nd observation, SG is seeds grown.

Vigor index (VI)
To determine VI, the number of seeds that exhibited normal germination on the first day of observation (day 3) was calculated using the following equation (Eq.5): where NS is normal seedlings; SG is seeds grown.

Germination rate (GR)
To measure GR, the number of days that each seed required to germinate was calculated using the following formula (Eq.6): where N = number of seeds germinating within each daily observation interval, T = length of time between the start of seeding and the end of the predetermined observation period.

Median germination time (T50)
T50 is the time required for the seeds to reach 50% germination of the total seeds sown by calculating the number of seeds grown every day.T50 describes seed vigor, calculated by the formula (Eq.7): where ti = intermediate time at or before seed germination 50%; tj = intermediate time after the seeds germinate 50%; n50% = number of seeds that germinated (50% of the total germinated seed); nj = number of seeds germinated at time tj; ti = number of seeds germinated at time ti.

Data analysis
This experiment was set up in a completely randomized design.The antagonistic ability test consisted of 5 treatments of Trichoderma sp.isolates, 1 synthetic fungicide treatment as a control (-) and 1 aquadest treatment as a control ( +).Meanwhile, the in-planta test also consisted of the same treatments as the antagonistic ability test, but only used a ( +) control.The experiment was repeated (7) T50 = ti + n50% − ni nj − ni tj − ti three times.Observational data were statistically tested using analysis of variance, and then the Least Significant Difference (LSD) test was performed at the 5% significance level (α = 0.05).

Results
Morphological characters of Trichoderma sp. and Rhizoctonia sp.
The macroscopic observation of Trichoderma sp.colonies showed a flat surface, rough-like fibrous, and smooth edges.The colony was initially white and the middle part was light green; then, the colony became dark green in the shape of a circle with clear boundaries, while the edges were white like cotton.The color of the colony turned into a dark green on the entire upper surface at 7 days old after being cultured (Fig. 1a).Meanwhile, the microscopic characters of Trichoderma sp. were greenish-white hyphae, short phialides stalk, greenish globoseshaped conidia that grew at the ends and some conidia formed clusters of light green color on the surface of the conidiophore cells.The conidiophore branches and phialides were ± 10 μm and ± 14 μm in length, respectively.Many conidiophore branches were like pyramids, namely the longer branches below, the phialides were arranged in different groups, and there were 2-3 phialides per group (Fig. 1b and c).Colony characters of Rhizoctonia sp. on PDA medium generally had white mycelium, then changed to yellowish white to dark brown (Fig. 4d).After several days of incubation on a PDA medium, the colonies produced sclerotia of various sizes and shapes (Fig. 4f ).While the microscopic character of Rhizoctonia sp., i.e., young hyphae had branches with an angle of 45 °C, the more mature the branches were perpendicular, stiff, and uniform.The color of the young hyphae was hyaline and the old hyphae were brown; the average diameter of the hyphae was around 6-10 μm (Fig. 4e).

Molecular characters of Trichoderma sp. and Rhizoctonia sp. based on the rDNA-ITS region
Morphological characters of Trichoderma sp.isolate and Rhizoctonia sp.isolates were verified by analyzing their molecular features through the use of universal primers ITS1/ITS4.The results of the molecular analysis indicated that PCR using ITS1/ITS4 primers successfully amplified DNA bands with a size of ± 500 bp for Trichoderma isolates (Fig. 2A) and ± 700 bp for Rhizoctonia sp.isolate RsHM (Fig. 2B).The ITS-1/ITS-4 primers were generally used to amplify fungal DNA in the internal transcribed spacer (ITS) region.This ITS region was a conserved, non-encoded functional protein located in the ribosomal DNA (rDNA) region.
According to the BLASTN 2.13.0 program, the sequencing analysis results indicated that all Trichoderma sp.isolates (HMRP7, HMRF7A, HMEDF1B, HMEDF6A, CHM01) had similarities to T. asperellum isolates from Nigeria, South Korea, Yogyakarta (Indonesia), China, and India with sequence identity matrix values of 1.000 (100% homology) (Table 1).Meanwhile, the RsHM isolate had similarities to R. solani isolates from Vietnam, Malaysia, China, Brazil, and India, with sequence identity matrix values ranging from 0.917 to 0.929 (homology 91.7-92.9%)(Table 2).The data of Blast result from NCBI showed the highest similarity (> 90%) with an e value of 0.0.
Phylogenetic analysis showed that Trichoderma sp.isolates (HMRP7, HMRF7A, HMEDF1B, HMEDF6A, CHM01) were closely related and grouped with T. asperellum isolate RCZID-3 from Nigeria, strain YS-27 from South Korea, strain PK1J2 from Yogyakarta (Indonesia), isolate Tasum66 from China, and isolate PAN-COM8 from India with a genetic distance coefficient of 0.000.Meanwhile, RsHM isolates were closely related and grouped with R. solani isolate RORC9 from India with genetic distance coefficient values ranging from 0.000 to 0.032.In addition, RsHM isolate was also quite closely related to R. solani strain 2773 from Brazil, JK-2016-14 isolates from China, CSU4 isolates from Malaysia, and CTCR02-2 isolates from Vietnam, with a genetic distance coefficient ranging from 0.002 to 0.007.Trichoderma sp.isolates were very distantly related to RsHM isolate, exhibiting a genetic distance coefficient of 0.448 (Fig. 3).

In vitro antagonistic ability of T. asperellum against R. solani
According to the dual culture method used for the antagonism test of T. asperellum isolates, all isolates were able to inhibit the growth of R. solani on PDA medium, with a percentage of inhibition ranging from 59.57 to 75.25%.However, the inhibition percentage with synthetic fungicide treatment/control ( − ) was only 56.54% (Fig. 4).The inhibition values of HMEDF6A and CHM01 isolates significantly differed from the control ( − ) in the LSD test level of 5% with 75.09 and 75.25% inhibition percentages, respectively.The LSD test at the 5% level revealed that the inhibitory abilities of the HMRP7, HMRF7A, and HMEDF1B isolates were non-significantly different from the control ( − ), with inhibition percentages of 72.17, 59.57, and 68.06%, respectively.
The antagonistic ability showed that the five T. aspereluum isolates could inhibit the growth of R. solani in vitro on PDA medium with an inhibition percentage of > 50% so that these isolates could be categorized as antagonistic agents.T. asperellum showed the antagonistic mechanism in inhibiting the growth and reproduction of R. solani through the parasitism mechanism.In this study, T. asperellum and R. solani formed antagonistic zone indicating competition and then hyperparasites.As the antagonist time progresses, R. solani mycelium gradually thins, stops growing, and does not form sclerotia (Fig. 5).

Inhibition effect of T. asperellum to mycelial growth of R. solani
The microscopic observations showed that there was a mechanism of parasitism/ hyperparasitism of T. asperellum against R. solani in PDA medium (Fig. 6).The T. asperellum mycelium spirally coiled around and penetrated the R. solani mycelium (Fig. 7b and c).This inhibitory mechanism led to cytoplasmic shrinkage and coagulation (Fig. 6d) and swollen malformation of R. solani mycelium (Fig. 6a).

In-planta ability of T. asperellum isolate to induce the growth of corn seedlings in green house
The ability of T. asperellum isolates to induce the growth of corn seedlings is shown in Table 3.The CHM01 isolate could generally induced corn seedlings' growth better than other isolates.CHM01 isolate had a better ability to increase GtR and VI compared to other isolates, where the GtR and VI values were 29.09and 47.22%, respectively, and significantly high the control treatment LSD test at a significance level of 5%.However, the CHM01 isolate had a lower GR value compared to other isolates.5% level found non-statistically significant difference between the isolates' MGP values.In the T50 variable, there was non-significant difference between all isolates and the control, but the T50 value of the CHM01 isolate was 4.65 days higher than the control which was only 4.47 days.
The LSD test at 5% level showed a significant difference between T. asperellum isolates for plant height and root length, but not for root number.HMEDF6A and CHM01 isolates increased plant height and root length by 60.97 and 118.59%, respectively, than the control, whereas in the variable number of roots, all isolates showed higher number of roots than the control, but not significantly different in the LSD test at the 5% level (Table 4).
The tested pathogen isolates, Rhizoctonia sp., showed traits with previously reported Rhizoctonia sp., such as the tendency of young colonies to be white and the ability of older isolates to change brown (Misawa and Kurose 2018).The difference in colony color is caused by pigments produced by pathogens in the media (Taheri et al. 2007).Sclerotia are categorized into macrosclerotia and microsclerotia based on size.Macrosclerotia typically measures 1 mm in diameter, are tiny, spherical, and blackish-brown in color (Lal and Kandhari 2009).Meanwhile, on PDA medium, microsclerotia takes on a form resembling thin crusts, which are small and white (Kumar et al. 2008).The size of the sclerotia was inconsistent, arranged in the center, the edges, scattered or even absent (Abdel-Sattar et al. 2017).
Using the universal primer pair ITS1/ITS4 to amplify the ITS region of ribosomal DNA (rDNA), species of Trichoderma sp. and Rhizoctonia sp.isolates were successfully identified.The HMRP7, HMRF7A, HMEDF1B, HMEDF6A, and CHM01 isolates were identified as T. asperellum with a genetic distance coefficient value of 0.000 and a very high similarity of 100%.With a homology level of > 90% and genetic distance coefficient values ranging from 0.000 to 0.032, the RsHM isolate was identified as R. to amplify the ITS region of ribosomal DNA (rDNA) because of its universal presence, conservative, and abundant presence.The rDNA is the coding region of the genome for the ribosomal DNA component.In addition, the ITS region also has a clear barcode to distinguish species and the presence of inter-and intra-specific nucleotide base variations (Buehler et al. 2017).
In vitro, antagonistic activity against R. solani was further assessed for the isolates HMRP7, HMRF7A, HMEDF1B, HMEDF6A, and CHM01.According to the test results, the five T. asperellum isolates had a > 50% ability to inhibit the growth of R. solani on PDA medium.The Trichoderma sp.antagonist mechanism was also reported by Harwoko et al. (2019) that the main biological control mechanism for Trichoderma species was hyperparasitism.Trichoderma sp.recognizes pathogens by identifying lectins secreted by pathogens (Kredics et al. 2018).Furthermore, Trichoderma sp.induces a series of hyperparasite-associated signaling pathways in its body (Stappler et al. 2017).
The mechanism of parasitism shown by T. asperellum in inhibiting the growth of R. solani, namely swollen malformation and hyphae coiling.R. solani's hyphae died due to the T. asperellum hyphae's coiling, penetration, and subsequent crushing of the hyphae into smaller fragments.The mechanism of Trichoderma sp.parasitism, in which the hyphae coiled on R. solani until haustoria formed inside the pathogenic hyphae, and then the haustoria took the cytoplasm until the hyphae shriveled (Jiang et al. 2016).According to Wu et al. (2017), T. asperellum utilizes a method of mycoparasitism whereby it wraps its hyphae around pathogens and secretes cell wall-degrading enzymes (CWDEs) containing chitinase, glucanase, and protease.The enzymes caused degradation of the cell walls in the pathogens, while the hyphae of Trichoderma sp.coiled around the hyphae of the pathogen, resulting in abnormalities in hyphal growth and lysis of pathogenic cells (Zhang and Zhuang 2020).
In addition to having antagonistic abilities, the five T. asperellum isolates also had the ability to enhance the growth of corn seed in planta.The results for the potential test of T. asperellum isolates in enhancing growth showed that the isolate was thought to produce bioactive compounds that could induce growth.A typical approach for promoting the growth of plants was through the capacity of T. asperellum isolates to colonize roots and supply them with nutrients and minerals.This study aimed to investigate the potential of T. asperellum as a saprophytic antagonist capable of enhancing corn seedlings.The growth of plants can be stimulated by T. asperellum, which was accomplished through the production of growth regulators (Glick 2012).A study reported by Hoyos-Carvajal et al. (2009) indicated out of 106 Trichoderma sp.isolates, 60% demonstrated the capacity to generate IAA and analogs of auxin.
Trichoderma sp. is a filamentous saprophytic fungus that lives in the rhizosphere and plant tissue, which can stimulate plant growth indirectly (Doni et al. 2014).The mechanism of Trichoderma sp. in enhancing growth is   In addition, there have been reports on various Trichoderma species that have the potential to influence plant growth and development directly.This includes promoting nutrient uptake, reducing reliance on synthetic chemical fertilizers, enhancing seed germination rates, and inducing plant resistance to biotic and abiotic stresses (Shoresh et al. 2010).

Conclusion
The five Trichoderma sp.isolates and Rhizoctonia sp.isolates were identified as T. asperellum and R. solani based on the characteristics of the ITS ribosomal DNA region by PCR technique.T. asperellum inhibited the growth of R. solani in vitro on PDA medium with a parasitism mechanism.CHM01 isolate showed better potential than other isolates in enhancing the growth of corn seedlings.
Further research is needed to confirm the results of laboratory and greenhouse tests so that the effectiveness of T. asperellum in controlling R. solani is known on a large scale in the field.

Fig. 7
Fig. 7 Effect of Trichoderma asperellum suspension soaking treatment on the corn growth in seedling trays.a HMRP7; b HMRF7A; c HMEDF6A; d HMEDF1B; e CHM01; f Control carried out by colonizing the rhizosphere zone of plant roots.The rhizosphere is influenced by the secretion of bioactive compounds in the form of highly hydrated mono-and di-saccharides by plant roots, which stimulate the growth and survival of fungi in the rhizosphere zone.Plant-derived sucrose serves as a carbon source for Trichoderma sp., which enables the colonization of roots, coordinates defense mechanisms, and enhances photosynthetic rates(Vargas et al. 2009).Several phytostimulatory mechanisms of Trichoderma sp. had been reported previously such as increased root development and production of auxin(Contreras-Cornejo et al. 2009), produce siderophores(Chowdappa et al. 2013), improved tolerance for drought(Shukla et al. 2012), defense protein expression in plants(Thakur et al. 2013), phosphate solubility(Saravanakumar et al. 2013), elicitor release(Nawrocka et al. 2013), and improved plant tolerance under conditions of salinity(Contreras-Cornejo et al. 2014).

Table 1
DNA sequence identity matrix of Trichoderma sp., which is juxtaposed with isolates in GenBank

Table 2
DNA sequence identity matrix of isolates of Rhizoctonia sp. which is juxtaposed with isolates in GenBank

Table 3
Effect of antagonistic fungi isolates on the vigor and viability of corn seed MGP maximum growth potential; GtR growth rate; GS growth simultaneity; VI vigor index; GR germination rate; T50 median germination time; LSD least significant difference; SE standard error; CV coefficient of variation Letters followed in the same column are not significantly different according to 5% LSD (α 0.05)

Table 4
The effect of antagonistic fungi isolates on the growth of corn seedling Letters followed in the same column are not significantly different according to 5% LSD (α 0.05) LSD least significant difference; SE standard error; CV coefficient of variation