First record of a parasitoid, Megaselia (M) scalaris (Diptera: Phoridae) of fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) from India

The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), has become a major threat in maize cultivation since its invasion to India in 2018. The humpbacked fly, Megaselia scalaris (Loew) (Diptera: Phoridae), was recorded as a laboratory parasitoid of FAW, for the first time in India. Initially, 30–40 maggots of M. (M) scalaris emerged out from the dead pre-pupa and pupa of laboratory-reared FAW. The fly laid up to 15 eggs on the outer surface of 6th instar larva or pre-pupa of the FAW. The incubation period was 1–2 days. The fly had 3 larval instars which lasted 3–4 days and a pupal period of 10–11 days. The adults survived for 6–7 days.


Background
Humpbacked flies or scuttle flies are the swift flying dipteran insects that belong to the family Phoridae. The species of this family like any other detritivores help to degrade the plant and animal matter (Disney 2008). The scuttle fly, Megaselia scalaris (Loew 1866), has been used as a model organism for biological control of insect pests. Its larval stage has been reported earlier as detritivores, facultative parasites, parasitoids, phytophagous, and coprophagous insect (Chakraborty et al. 2016). M. scalaris has been documented in many crops against various pests around the world (Harrison and Gardner 1991). Recently, the wide distribution of the fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), has attracted many natural enemies. Among them, order Diptera serves as a natural controlling agent. In the year 2003, the survey's carried out in the America and Caribbean island reported Megaselia sp. as one of the larval parasitoid infesting late-instar larvae of FAW (Molina et al. 2003). Further, in Mexico, during 2007, natural exploration recorded M. scalaris as a unique parasitoid among the natural enemies documented on FAW in Mexico (Ruı´z-Na´jera et al. 2007).
The FAW is a notorious trans-boundary pest native to tropical and subtropical America (Sparks 1979). In January 2016, it was reported in Africa and spread to 44 countries in sub-Saharan Africa (Prasanna et al. 2018); later, it invaded India in May 2018 causing serious damage to maize plants ) and subsequently spread to southern states (Mahadevaswamy et al. 2018

Methods
The parasitoid was recorded for the first time in India from a field population of FAW, collected from Shivamogga district of Karnataka, near a village named Kudurekonda located 14.1365°N and 75.5306°E, during September, 2020. The field collected larvae were reared under laboratory conditions at a temperature of 25°C and 75-80% RH. Later, adult parasitoids were noticed emerging out from the pupae of these field-collected larvae. The adult parasitoids were stored in 70% ethanol for identification at Diptera Section, ZSI, Kolkata. The biology of M. scalaris was studied on 5th or 6th instar larvae of FAW under laboratory conditions. Initially, the pupae of the parasitoid were collected from the infested culture of FAW. After adults' emergence from the pupae, a pair of adult parasitoids were released into 3 individual plastic boxes separately, each containing 5 head crushed (to avoid cannibalism) 6th instar FAW larvae, 5 pre-pupa, and 5 pupae, respectively. The parasitoid adults were provided 10% honey solution as food. Further, rearing of the parasitoids was carried out as per methodology adopted by Chakraborty et al. (2016).
The adult fly was yellowish brown with many long bristles; the supra-antennal bristles were equidistant. The thorax was brownish dorsally, the prothoracic pleura had many small hairs and 2-4 long ventral bristles; the mesopleuron was lacking distinctive bristles, the scutum had indistinct brown markings having a pair of long bristles at the posterior margin; the scutellum had 2 pairs of bristles; legs were paler; the femur was enlarged, it was darker apically with dark brown small hairs, halters creamy yellowish, wings with half of the costal margin densely fringed; and the abdomen was darker brown (Fig. 1f, g).

Preliminary biological data of Megaselia scalaris (Loew)
As many as 30-40 maggots emerged out of the dead pre-pupae (Fig. 1a) and pupae of FAW. Two days post release, the oviposition by the parasitoids was observed on the outer surface of late-instar larvae in which they are about to pupate and also on pre-pupae and pupae. The adult fly laid pearl white coloured eggs of about 1-15 on the outer surface of the FAW 6th instar larvae (Fig. 1b) and pre-pupae (Fig. 1c). One to 2 days after egg-laying, creamy white maggots emerged. Three larval instars were recorded by mean durations of 1-2 days each (Fig. 1d). The pupa is of coarctate type, light brown in colour, with 2 pairs of spiracles observed on each end of the pupa (Amphineustic). Another interesting structure on the male and female pupae was the presence of respiratory tube or thoracic horn. The pupal period ranged from 10 to 11 days. Male and female pupae could be distinguished based on size (Fig. 1e). Usually, adult females were larger than the males. The fed adults' longevity on 10% honey solution ranged 6-7 days under laboratory conditions.

Discussion
This study is a new record of M. scalaris as a parasitoid of FAW in India; however, it has already been reported to infest FAW from the neighbouring country China (Tang et al. 2021). Eggs were found on the outer surface of 6th instar larvae or pre-pupae of FAW. Benner (1985) reported the female fecundity of about 1-100 matured eggs in one batch. In the present study, the female laid 1-15 eggs. The present study reported the emergence of 30-40 maggots from the pre-pupa and dead pupa of FAW. Similarly, El-Hawagry et al. (2021) had observed the emergence of 40-50 larvae per individual of parasitized Green stink bug, Nezara viridula. Three larval instars of M. scalaris were recorded, which are in line with the findings of Kaneko et al. (1978). The pupal period in the present study ranged from 10 to 11 days, which are dissimilar with findings of Benner and Ostermeyer (1980) who reported that male larvae of M. scalaris pupate 2 days earlier than the female larvae at 25°C. Apart from the size, males and females can also be differentiated based on the modification of the last abdominal segment, i.e. narrow extension of 6th abdominal tergite (in case of female), and distinct terminalia that was easily distinguished in case of males (Brown and Oliver 2007).

Conclusion
The present study is just a new record of the parasitoid M. scalaris on FAW in India. Further studies on its parasitization of M. scalaris under laboratory and field conditions are needed.