Multi-species endophytic Bacillus for improved control of potato soilborne and tuber-borne diseases in Tunisia: from laboratory to field conditions

Egyptian Journal of Biological Pest Control

Table 2 Growth inhibition rate (%) induced by Bacillus spp. cell-free culture filtrates tested at two concentrations against three potato wilt-causing fungi

Bacterial treatment	Concentration (%)	Fungi tested
Bacterial treatment	Concentration (%)	Fusarium oxysporum f. sp. tuberosi	Rhizoctonia solani	Colletotrichum coccodes	Average
Bacillus tequilensis SV39	10	8.72	14.93	34.92	19.52 cd^d*
	20	40.87	33.80	42.76	39.14 a
	Average^c*	24.80 a⁺	24.37 a	38.84 a	29.33 A^a*
B. subtilis SV41	10	7.85	17.75	19.59	15.06 e
	20	9.74	23.94	23.68	19.12 d
	Average	8.80 c	20.85 b	21.64 c	17.09 C
B. cereus S42	10	7.85	14.65	22.32	14.94 e
	20	19.85	22.82	31.52	24.73 b
	Average	13.86 b	18.73 bc	26.92 b	19.83 B
B. methylotrophicusSV44	10	9.74	12.68	18.23	13.55 e
	20	19.42	21.13	27.09	22.54 bc
	Average	14.58 b	16.90 c	22.66 bc	18.04 BC
B. amyloliquefaciens subsp. plantarum SV65	10	12.37	10.42	20.95	14.58 e
	20	19.42	22.25	31.86	24.51 b
	Average	15.90 b	16.34 c	26.41 b	19.54 B
B. tequilensis SV104	10	11.49	14.93	14.48	13.63 e
	20	21.19	21.69	28.45	23.77 b
	Average	16.35 b	18.31 bc	21.47 c	18.7 BC
Average^b*		15.71 c	19.25 b	26.32 a

^aMean mycelial growth inhibition per bacterial treatment for all concentrations and all fungal pathogens combined
^bMean mycelial growth inhibition per fungal pathogen for all bacterial treatments and all concentrations combined
^cMean mycelial growth inhibition per bacterial treatment and per fungal pathogen for all concentration combined
^dMean mycelial growth inhibition per bacterial treatment per concentration for all fungal pathogens combined
+ Means within columns followed by the same letter are not significantly different according to Duncan's multiple range test at P ≤ 0.05
*For fungal pathogens, bacterial treatments tested and concentrations applied, values (means) followed by the same letter are not significantly different according to Duncan's multiple range test at P ≤ 0.05
LSD (Fungal pathogens × Bacterial treatments × Cell-free culture filtrate concentrations) = 5.13% at P ≤ 0.05
Growth inhibition was noted after incubation at 25 °C for 3 days (R. solani), 7 days (F. oxysporum f. sp. tuberosi) and 8 days (C. coccodes) on PDA medium