Table 1 PCR program for ITS gene detection of the studied fungi
From: Evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot disease
Steps | Temperature (°C) | Time (M:S) | No. of cycle |
|---|---|---|---|
Initial denaturation | 95 | 03:00 | 1 |
Denaturation | 95 | 00:45 | 35 |
Annealing | 58 | 00:45 | |
Extension | 72 | 00:45 | 1 |
Final incubation | 72 | 07:00 |