Table 1 Calibrators used for qualitative ELISA
From: Cry toxin expression in different plant parts of Bt cotton at different phenological stages
S. no. | Cry1Ac calibrators (ready to use) | Cry2Ab calibrators (ready to use) |
|---|---|---|
1 | Cry1Ac mAb-coated 96-well ELISA plate | Cry2Ab mAb-coated 96-well ELISA plate |
2 | Anti-Cry1Ac conjugate | Anti-Cry2Ab conjugate |
3 | Cry1Ac positive control | Cry2Ab positive control |
4 | Negative control | Negative control |
5 | 10 × Extraction buffer | 10 × Extraction buffer |
6 | 10 × Wash buffer | 10 × Wash buffer |
7 | TMB substrate (HRP) | TMB substrate (HRP) |
8 | Stop solution | Stop solution |