Table 2 Biotoxicity assays of Cry1Ac and Cry2Aa mutant proteins against 2nd instar larvae of Spodoptera litura
Treatments (µg/g) | LC50 valuesa | 95% Confidence limits | Slope ± SE | X2 | |
|---|---|---|---|---|---|
LL | UL | ||||
Cry1Ac/pGEM | 248.120 | 178.089 | 607.361 | 1.608 ± .476 | 0.553 |
Cry1-L588A (M1) | 249.842 | 185.649 | 514.620 | 1.856 ± .506 | 1.257 |
Cry1-L588A (M2) | 249.294 | 193.585 | 429.468 | 2.269 ± .556 | 1.09 |
Cry1-L588A (M3) | 249.539 | 191.650 | 443.347 | 2.152 ± .534 | 0.675 |
Cry1-S573A (M1) | 248.528 | 180.710 | 568.758 | 1.692 ± .486 | 0.553 |
Cry1-S573A (M2) | 248.485 | 183.132 | 533.895 | 1.785 ± .499 | 1.741 |
Cry1-S573A (M3) | 248.669 | 189.444 | 456.132 | 2.065 ± .525 | 0.439 |
Cry2Aa/pGEM | 169.195 | 121.185 | 302.855 | 1.450 ± .442 | 0.320 |
Cry2-Thr325A (M1) | 168.763 | 126.397 | 265.461 | 1.672 ± .452 | 0.157 |
Cry2-Thr325A (M2) | 168.720 | 121.243 | 298.467 | 1.464 ± .442 | 0.359 |
Cry2-Thr325A (M3) | 168.055 | 133.038 | 295.122 | 1.646 ± .453 | 0.565 |
Cry2-S445A (M1) | 167.581 | 130.924 | 237.658 | 1.974 ± .466 | 0.408 |
Cry2-S445A (M2) | 167.232 | 126.704 | 254.069 | 1.746 ± .454 | 0.581 |
Cry2-S445A (M3) | 167.628 | 135.007 | 223.489 | 2.280 ± .490 | 1.023 |