Table 1 Specifically designed chimeric primers for overlap extension PCR of cry1Ac and cry2Aa genes
Primer name | Sequence 5′-3′ |
|---|---|
cry1Ac-pGEM-1F | GACCCAGGGTGTTGGGTACCGAGCTCGAATTCGCCC |
cry1Ac-pGEM-2R | AGGATGTTTTTGTCGGGGATCCTCTAGAATCGAATTCCCGCGGCCG |
cry1Ac-Ser-1F | CCGCGACTATCAATGCGCGTTATAATG |
cry1Ac-Ser-2R | CATTATAACGCGCATTGATAGTCGCGG |
cry1Ac-Leu-1F | CGTTATAATGATGCGACTAGGCTTATTGGC |
cry1Ac-Leu-2R | GCCAATAAGCCTAGTCGCATCATTATAACG |
cry1Ac insert-vect-1F | GGGCGAATTCGAGCTCGGTACCCAACACCCTGGGTC |
cry1Ac-insert-vect-2R | CGGCCGCGGGAATTCGATTCTAGAGGATCCCCGACAAAAACATCCT |
cry2Aa-pGEM-1F | CAATACATTATTCATATAAAATTCCTCCTTAATCGAATTCCCGCGGCCG |
cry2Aa-pGEM-2R | CTTCCACCACTTTATTAAGGTTTGAGTGAATCACTAGTGAATTCGCG |
cry2Aa-Thr-1F | CTTAATACAGATGCGCTTGCTCGTG |
cry2Aa-Thr-2R | CACGAGCAAGCGCATCTGTATTAAG |
cry2Aa-Ser-1F | CAATAACTTCTGCGGTTAATACAATGC |
cry2Aa-Ser-2R | GCATTGTATTAACCGCAGAAGTTATTG |
cry2A-insert-vect-1F | CGGCCGCGGGAATTCGATTAAGGAGGAATTTTATATGAATAATGTATTG |
cry2A-insert-vect-2R | CGCGAATTCACTAGTGATTCACTCAAACCTTAATAAAGTGGTGGAAG |
cry2A-seq’P | GCTGCTGAAATACCCCATTCATCTGC |
cry1Ac-seq’P | CGGGAACAGAGCAACGATATCTAATACAG |