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Egyptian Journal of Biological Pest Control
Fig. 1 | Egyptian Journal of Biological Pest Control

Fig. 1

From: Site-directed mutagenesis in Cry proteins of Bacillus thuringiensis to demonstrate the role of domain II and domain III in toxicity enhancement toward Spodoptera litura

Fig. 1

Site-directed mutagenesis PCR for cry1Ac and cry2Aa full-length genes through KOD Plus mutagenesis kit A Amplification of cry1Ac 4.1 kb gene and amplified vector (pGEM) 3 kb B Amplification of cry2Aa 1.9 kb gene and amplified vector (pGEM) 3 kb C SDS-PAGE analysis for Cry1Ac non-mutant and mutant proteins expressed in Escherichia coli. D SDS-PAGE analysis for Cry2Aa non-mutant and mutant proteins

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