Table 1 Different types of insect cell culture media
Media | Formulation | References |
|---|---|---|
Conventional media | ||
Wyatt’s medium | Medium consisted of high concentrations of organic acids, amino acids, inorganic salts and sugars supplemented with heat-treated hemolymph | Wyatt et al. (1956) |
Grace medium | Medium consisted of 21 amino acids, four organic acids (Krebs cycle intermediates), ten vitamins, two antibiotics, six salts, three sugars, and insect plasma | Grace (1962) |
MM insect culture medium | Medium consisted of only 6 salts, yeastolate, fetal bovine serum (FBS), lactalbumin hydrolysate, glucose, and antibiotics | Mitsuhashi (1964) |
Hink’s TNM-MH medium | Medium consisted of lactalbumin, yeastolate as source of vitamins (B-complex), and hemolymph with heat-inactivated fetal bovine serum | Hink (1970) |
BML-TC/10 | Medium consisted of glucose (only hexose sugar), fetal bovine serum and tryptose extract | Schlaeger (1996) |
IPL-41 medium | Medium consisted of increased concentration of amino acids, vitamins and protein hydrolysates (lactalbumin, tryptose phosphate broth, yeastolate) | Goodwin (1975) |
Serum media | ||
ISFM medium | Prepared based on IPL-41, ultrafiltered yeastolate (4g1−1) and a complex lipid emulsion were added | Inlow et al. (1989) |
Ex-Cell-400 medium | It’s a semi-defined medium with a protein concentration of 15 mg/m1−1 or less | Belisle et al. (1992) |
ExCeU 401 medium | It’s a protein-free medium that allows for higher cell density and higher yields of expressed recombinant proteins | Schlaeger (1996) |
Sf 900 medium | It’s a medium with low protein content | Weiss et al. (1992) |