Fig. 3

Phylogenetic analysis for DNA gyrase gene of 12 Bacillus thuringiensis strains. Evolutionary relationships of taxa. The evolutionary history was inferred using the neighbor-joining method [1]. The optimal tree with the sum of branch length = 0.68590967 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [2]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Kimura two-parameter method [3] and are in the units of the number of base substitutions per site. The analysis involved 41 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + noncoding. All positions containing gaps and missing data were eliminated. There were a total of 119 positions in the final dataset. Evolutionary analyses were conducted in MEGA7 [4]