Table 3 SDS-PAGE electrophoretic-gram data analysis for effect of Trichoderma harzianum, some salts and antioxidants on protein pattern of faba bean plants against root rot infection
Band No | RF | M. Wt | Bands intensity of different treatments | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Vitamin E + vitamin C + enervit + selenium + vitamax plus | Trichoderma harzianum + vitamin E + vitamin C + enervit + selenium + vitamax plus | T. harzianum (108 spores/ml) | Rizolex-T (50WP) (1 gm/l) | Untreated control | ||||||||
1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | |||
1 | 0.044 | 160 | – | – | 616 | 485 | 405 | – | 702 | 618 | 730 | 356 |
2 | 0.076 | 148 | 2274 | 2086 | – | 410 | 682 | – | – | 441 | 422 | 427 |
3 | 0.096 | 140 | – | – | – | – | – | – | 566 | 567 | – | – |
4 | 0.111 | 125 | – | – | – | – | 459 | 545 | – | – | 457 | 332 |
5 | 0.235 | 80 | – | 1941 | – | – | 632 | 480 | 790 | 894 | 927 | 420 |
6 | 0.332 | 70 | – | – | 430 | 569 | – | – | – | – | – | – |
7 | 0.345 | 60 | 2277 | 1771 | – | – | – | – | – | – | – | – |
8 | 0.411 | 52 | – | – | – | – | – | – | – | – | – | – |
9 | 0.482 | 43 | 1791 | 2340 | 1705 | 1335 | 1463 | 1361 | 1070 | 651 | 644 | 799 |
10 | 0.513 | 30 | 2004 | 1237 | 530 | 726 | 1664 | 1465 | 1361 | 1176 | – | – |
11 | 0.599 | 28 | 2192 | 2035 | 770 | 448 | – | – | 1101 | 1095 | 953 | 801 |
12 | 0.685 | 20 | 1894 | 2032 | – | – | 834 | 728 | – | – | 795 | 421 |
13 | 0.724 | 18 | – | – | 674 | 674 | – | – | 1067 | 851 | 805 | 479 |
14 | 0.876 | 15 | 2398 | – | 430 | 430 | 370 | 701 | 697 | 705 | 581 | 722 |
No. of bands | 7 | 7 | 7 | 8 | 8 | 6 | 8 | 9 | 9 | 9 | ||
8 | 8 | 8 | 9 | 9 | ||||||||
New proteins after 2nd irrigation | 1 | 1 | – | 1 | – | |||||||
Deleted proteins after 2nd irrigation | 1 | – | 2 | – | – | |||||||
More intensive proteins in 2nd irrigation than 1st one | 2 | 2 | 3 | 1 | 2 | |||||||
More intensive proteins in 1st irrigation than 2nd one | 4 | 3 | 3 | 5 | 6 | |||||||
Proteins with same intensity after both irrigations | – | 2 | – | 2 | 1 | |||||||
Band No | RF | M. Wt | Bands intensity of different treatments | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Disodium phosphate | Calcium sulfate | Potassium metabisulfite | Sodium sulfate | Cysteine | ||||||||
1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | |||
1 | 0.044 | 160 | – | – | – | – | – | – | – | – | – | – |
2 | 0.076 | 148 | – | – | – | – | – | – | – | – | – | – |
3 | 0.096 | 140 | – | – | – | – | – | – | – | – | – | – |
4 | 0.111 | 125 | – | – | – | – | – | – | – | – | – | – |
5 | 0.235 | 80 | 671 | – | – | 1120 | 716 | 1179 | 798 | 1018 | 597 | 955 |
6 | 0.332 | 70 | – | – | – | – | – | – | – | – | – | 744 |
7 | 0.345 | 60 | 1144 | – | – | 1242 | – | – | – | 1055 | – | – |
8 | 0.411 | 52 | 1085 | 632 | 977 | 1659 | – | – | 1372 | 1260 | 868 | 1423 |
9 | 0.482 | 43 | – | 1641 | 1871 | 3163 | 1359 | 1755 | – | 1842 | – | – |
10 | 0.513 | 30 | 2431 | 1485 | 1480 | 1780 | 2344 | 2797 | 2034 | 2200 | 1607 | 1879 |
11 | 0.599 | 28 | 1720 | 1326 | 1123 | 2595 | 1632 | 2473 | 1466 | 1460 | 1084 | 1805 |
12 | 0.685 | 20 | 1540 | – | – | 2091 | 1479 | 1976 | 1784 | 942 | 827 | 1159 |
13 | 0.724 | 18 | 2081 | – | – | – | – | 1564 | 1075 | 1322 | 1527 | 1899 |
14 | 0.876 | 15 | – | 1509 | 2307 | 3379 | 2203 | 2664 | – | 969 | – | – |
No. of bands | 7 | 5 | 5 | 8 | 6 | 7 | 6 | 9 | 6 | 7 | ||
9 | 8 | 7 | 9 | 7 | ||||||||
New proteins after 2nd irrigation | 2 | 3 | 1 | 3 | 1 | |||||||
Deleted proteins after 2nd irrigation | 4 | – | – | – | – | |||||||
More intensive proteins in 2nd irrigation than 1st one | – | 5 | 6 | 3 | 6 | |||||||
More intensive proteins in 1st irrigation than 2nd one | 3 | – | – | 2 | – | |||||||
Proteins with same intensity after both irrigations | – | – | – | 1 | – | |||||||
Band No | RF | M. Wt | Bands intensity of different treatments | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Potash alum | Cysteine + potash alum | Cysteine + Trichoderma harzianum | Potash alum + T. harzianum | Cysteine + potash alum + T. harzianum | ||||||||
1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | |||
1 | 0.044 | 160 | – | – | – | – | – | – | – | – | – | – |
2 | 0.076 | 148 | – | – | – | – | – | – | – | – | 1601 | 1754 |
3 | 0.096 | 140 | – | – | – | – | – | – | – | – | – | – |
4 | 0.111 | 125 | 1895 | 1594 | – | – | 2056 | 1646 | 1756 | 1639 | – | – |
5 | 0.235 | 80 | – | – | 1449 | 3574 | – | – | – | – | 1159 | 1524 |
6 | 0.332 | 70 | – | – | – | 2585 | – | – | – | – | – | – |
7 | 0.345 | 60 | – | – | 1623 | – | – | – | – | – | – | – |
8 | 0.411 | 52 | – | – | 1954 | 3737 | – | – | – | – | 1212 | 2524 |
9 | 0.482 | 43 | 1837 | 1629 | 3334 | 3446 | 2130 | 2143 | 2370 | 1761 | – | – |
10 | 0.513 | 30 | – | – | 2229 | 3916 | – | – | – | – | – | – |
11 | 0.599 | 28 | – | – | 2232 | 3114 | 4053 | 3644 | 3063 | 2162 | 1826 | 2393 |
12 | 0.685 | 20 | 2638 | 1926 | – | – | 1801 | 2519 | 1891 | 2102 | 2153 | 2251 |
13 | 0.724 | 18 | 1836 | 1451 | 2968 | 3254 | 1361 | 1759 | 2161 | 1752 | 1727 | 2743 |
14 | 0.876 | 15 | 1705 | 1556 | – | 2392 | – | – | – | – | 1290 | 2681 |
No. of bands | 5 | 5 | 7 | 8 | 5 | 5 | 5 | 5 | 7 | 7 | ||
5 | 9 | 5 | 5 | 7 | ||||||||
New proteins after 2nd irrigation | – | 2 | – | – | – | |||||||
Deleted proteins after 2nd irrigation | – | 1 | – | – | – | |||||||
More intensive proteins in 2nd irrigation than 1st one | – | 6 | 2 | 1 | 7 | |||||||
More intensive proteins in 1st irrigation than 2nd one | 5 | – | 2 | 4 | – | |||||||
Proteins with same intensity after both irrigations | – | – | 1 | – | – | |||||||
Band No | RF | M. Wt | Bands intensity of different treatments | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enervit | Selenium | Vitamin E | Vitamin C | Vitamax plus | ||||||||
1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | |||
1 | 0.044 | 160 | – | – | – | – | – | – | 494 | 235 | – | – |
2 | 0.076 | 148 | 2360 | 2223 | 2490 | 1965 | 1346 | 2659 | – | – | 1984 | 2527 |
3 | 0.096 | 140 | – | – | – | – | – | – | – | 486 | – | – |
4 | 0.111 | 125 | – | – | – | – | – | – | – | – | – | – |
5 | 0.235 | 80 | – | 1779 | 1215 | 1787 | – | 1369 | – | 350 | – | – |
6 | 0.332 | 70 | – | – | – | – | – | – | 723 | 594 | – | – |
7 | 0.345 | 60 | 2397 | 2129 | 2250 | 2182 | 1505 | 2383 | – | – | 985 | 2242 |
8 | 0.411 | 52 | – | – | – | – | – | – | – | – | – | – |
9 | 0.482 | 43 | – | – | – | – | 2542 | 2092 | 1715 | 2217 | 2634 | 3103 |
10 | 0.513 | 30 | 2924 | 2922 | 1947 | 3628 | 1591 | 1785 | 765 | 2217 | 1199 | 2136 |
11 | 0.599 | 28 | – | – | 1967 | 2382 | 1692 | 2601 | 763 | 1374 | 2038 | 2355 |
12 | 0.685 | 20 | 2466 | 2205 | 1623 | 2320 | 1499 | 1966 | – | – | 2205 | 2595 |
13 | 0.724 | 18 | 2563 | 2285 | – | – | – | – | 846 | 598 | – | – |
14 | 0.876 | 15 | 2209 | 1998 | 2003 | 2311 | 1305 | 2755 | 704 | 633 | 2082 | 2755 |
No. of bands | 6 | 7 | 7 | 7 | 7 | 8 | 7 | 9 | 7 | 7 | ||
7 | 7 | 8 | 9 | 7 | ||||||||
New proteins after 2nd irrigation | 1 | – | 1 | 2 | – | |||||||
Deleted proteins after 2nd irrigation | 1 | – | – | – | – | |||||||
More intensive proteins in 2nd irrigation than 1st one | – | 5 | 6 | 3 | 7 | |||||||
More intensive proteins in 1st irrigation than 2nd one | 5 | 2 | 1 | 4 | – | |||||||
Proteins with same intensity after both irrigations | 1 | – | – | – | – | |||||||
Band No | RF | M. Wt | Bands intensity of different treatments | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Trichoderma harzianum + enervit | T. harzianum + selenium | T. harzianum + vitamin E | T. harzianum + vitamin C | T. harzianum + vitamax plus | ||||||||
1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | |||
1 | 0.044 | 160 | – | – | – | – | – | – | 621 | 565 | 376 | 502 |
2 | 0.076 | 148 | 592 | 766 | 517 | 859 | – | 688 | – | – | 284 | – |
3 | 0.096 | 140 | – | 611 | – | – | – | – | 730 | 582 | – | – |
4 | 0.111 | 125 | – | – | – | 494 | 581 | 587 | – | – | – | – |
5 | 0.235 | 80 | – | 771 | – | 658 | – | 541 | 498 | 444 | – | – |
6 | 0.332 | 70 | – | – | – | – | – | – | 1210 | 854 | 263 | 373 |
7 | 0.345 | 60 | – | 1390 | 954 | 777 | 537 | 912 | – | – | – | – |
8 | 0.411 | 52 | 505 | – | – | – | – | – | – | – | – | – |
9 | 0.482 | 43 | 410 | 729 | – | – | – | – | 2289 | 2022 | 1087 | 1211 |
10 | 0.513 | 30 | 448 | 2114 | 2670 | 1888 | 1988 | 2409 | 2289 | 941 | 708 | 499 |
11 | 0.599 | 28 | 503 | 1781 | 1012 | 1594 | 1077 | 2566 | 1606 | 878 | 462 | 465 |
12 | 0.685 | 20 | 819 | 1538 | 1021 | 1326 | 1295 | 1773 | – | – | – | – |
13 | 0.724 | 18 | 750 | 736 | 1070 | 975 | 1040 | 1186 | 562 | 1222 | 819 | 826 |
14 | 0.876 | 15 | 479 | 1486 | 1626 | 1241 | 1672 | 1425 | 1292 | 1294 | 345 | 410 |
No. of bands | 8 | 10 | 7 | 9 | 7 | 9 | 9 | 9 | 8 | 7 | ||
11 | 9 | 9 | 9 | 8 | ||||||||
New proteins after 2nd irrigation | 3 | 2 | 2 | – | – | |||||||
Deleted proteins after 2nd irrigation | 1 | – | – | – | 1 | |||||||
More intensive proteins in 2nd irrigation than 1st one | 7 | 4 | 6 | 1 | 4 | |||||||
More intensive proteins in 1st irrigation than 2nd one | – | 3 | – | 7 | 1 | |||||||
Proteins with same intensity after both irrigations | 1 | – | 1 | 1 | 2 | |||||||