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Table 1 Composition of artificial diet for mass rearing and bioassay for the pink bollworm, Pectinophora gossypiella (1000 ml)

From: Response of pink bollworm Pectinophora gossypiella (Saunders) to Cry1Ac and Cry2Ab toxin

Ingredients

Pectinophora gossypiella

Rearing

Bioassay

PART A

 Kabuli gram flour

30 g

 Southland diet-Multi Sp

30 g

60 g

 R.O. Water

400 ml

400 ml

PART B

 Agar Agar

14 g

14 g

 R.O. water

400 ml

400 ml

PART C micro-ingredients and antimicrobials

 Wesson’s salt

2.5 g

2.5 g

 Casein

5 g

5 g

 Cholesterol

0.5 g

0.5 g

 Cysteine

0.1 g

0.1 g

 Ascorbic acid

2 g

2 g

 Multivitamin mix (Polybion SF)

6 ml

6 ml

 Sorbic acid

0.5 g

0.5 g

 Methyl parahydroxy benzoate (MPHP)

0.5 g

0.5 g

 Streptomycin sulfate

0.5 g

0.5 g

 Bavistin

2.5 g

2.5 g

 Cifran (Ciproflaxacin injection)

3 ml

3 ml