From: Molecular profiling of resistance alleles in Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) collected from different locations
Cycle
Denaturation
Annealing
Extension
First cycle
94° C
5 min
–
2–40 cycles
20 s
55° C
1 min
72° C
Last cycle
7 min