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Table 2 Different cycles of DNA amplification of pink bollworm population through PCR Primers

From: Molecular profiling of resistance alleles in Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) collected from different locations

Cycle

Denaturation

Annealing

Extension

First cycle

94° C

5 min

2–40 cycles

94° C

20 s

55° C

1 min

72° C

1 min

Last cycle

55° C

1 min

72° C

7 min