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Fig. 3 | Egyptian Journal of Biological Pest Control

Fig. 3

From: Possible repair of UV-damaged Spodoptera littoralis nucleopolyhedrovirus DNA via generation of mutant carrying photolyase gene with DNA repair activity

Fig. 3

A stained agarose gels show the verification of different selected colonies upon transformation of pFB-ie2-GFP-ie1-phr plasmid into DH10β cells carrying Splibac. The upper agarose gel shows High Molecular Weight (HMW) genomic DNA isolated from 8 screened colonies. The lower agarose gel shows amplification of phr gene ORF of eight selected colonies using phr-F and phr-R primers. M: 1 kbp DNA ladder. Arrow shows a clear band of 1.4 kbp corresponding to the phr gene ORF

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