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Egyptian Journal of Biological Pest Control
Fig. 1 | Egyptian Journal of Biological Pest Control

Fig. 1

From: Possible repair of UV-damaged Spodoptera littoralis nucleopolyhedrovirus DNA via generation of mutant carrying photolyase gene with DNA repair activity

Fig. 1

Schematic representation shows construction of the recombinant vSpli-phr harboring the phr gene ORF. a A total of 1% agarose gel shows the amplified photolyase gene ORF (phr) of 1.4 kpb. Lane (−ve): PCR negative control. M: 1 kb ladder. b Plasmid map shows the phr gene that was cloned downstream from the ie-1 promoter of the modified pFastBac-Dual vector generating the hybrid plasmid PFB-ie2-GFP-ie1-phr of about 8.8 kb. c Transposition of the phr cassette into the attTn7 transposition site of the Splibac in the presence of the helper plasmid. Dotted line circle shows the phr cassette

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