Possible mechanisms of action of Bacillus wiedmannii AzBw1, a biocontrol agent of the root-knot nematode, Meloidogyne arenaria

Egyptian Journal of Biological Pest Control

Table 2 Hydrolytic activity and nematicidal effect of dialyzed sample before loading on column and non-bonded molecules (flow-through) from anion exchange chromatography

	Protein concentration (µg/ml)	Chitinase activity (U mL⁻¹)	Protease activity (U mL⁻¹)	Juvenile mortality (%)	Egg hatching (%)
Dialyzed sample before loading on column*	762 ± 12.32	7.2 ± 2.10 U mL⁻¹	56.65 ± 8.54	38.50 a	20.25 b
Flow-through	77.5 ± 5.56	8.40 ± 1.86 U mL⁻¹	2.33 ± 0.54 U mL⁻¹	22 b	68.50 a
Buffer**	–	–	–	17.50 b	67.55 a
Distilled water	–	–	–	10.75 c	66.75 a

Values followed by the same letter are not significantly different at 1%, as determined by analysis of variance followed by Fisher’s test. This experiment was conducted twice with the same results
^*Protein sample was prepared by acetone precipitation of secretory proteins from medium culture of AzBw1 and dialyzed against 20 mM Tris buffer
^**500 mM NaCl in 20 mM Tris buffer (pH 8.0)