Possible mechanisms of action of Bacillus wiedmannii AzBw1, a biocontrol agent of the root-knot nematode, Meloidogyne arenaria

Egyptian Journal of Biological Pest Control

Table 1 Nematicidal effect of cells suspension and filtered medium culture of strain AzBw1

	Washed cells	Control	Filtered bacterial culture	Control (medium culture)
Juvenile mortality	82.5 a	23.5 b	58.58 a	15.58 b
Egg hatching	14.75 b	63 a	29.28 b	59.71 a

After growing AzBw1 in LB medium, grown cells were harvested by centrifugation and washed twice with distilled water before using for bioassay. The supernatant was filtered by 0.22 µM syringe filter. One-half ml of this solution was mixed with either same volume of eggs or juvenile suspensions (containing 100 of each) and hatching of eggs and juvenile’s mortality were assessed for 6 days. Values followed by the same letter are not significantly different at 1%, as determined by analysis of variance followed by Fisher’s test. This experiment was conducted twice with the same results