Table 1 Primers used in this study and their sequences
Gene region | Primer name | Sequence (5′→3′) | References |
|---|---|---|---|
ITS1-5.8S-ITS2 | ITS4 | 5′-TCCTCCGCTTATTGATATGC-3′ | White et al. (1990) |
ITS5 | 5′-GGAAGTAAAAGTCGTAACAAGG-3′ | ||
Tef (EF1-α) | EF1T | 5′-ATGGGTAAGGARGACAAGAC-3′ | Rehner and Buckley (2005) |
1567R | 5′-ACHGTRCCRATACCACCSATCTT-3′ | ||
Bloc | B5.1F | 5′-CGACCCGGCCAACTACTTTGA-3′ | Rehner et al. (2006) |
B3.1R | 5′-GTCTTCCAGTACCACTACGCC-3′ | ||
RPB1 | RPB1Af | 5′-GARTGYCCDGGDCAYTTYGG-3′ | Stiller and Hall (1997) |
RPB1C | 5′-CCNGCDATNTCRTTRTCCATRTA-3′ | ||
β-Tubulin | T1 | 5′-AACATGCGTGAGATTGTAAGT-3′ | O’Donnell and Cigelnik (1997) |
T2 | 5′-TCTGGATGTTGTTGGGAATCC-3′ |