Biocontrol potential of native entomopathogenic nematodes against coconut rhinoceros beetle, Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) under laboratory conditions

Egyptian Journal of Biological Pest Control

Table 2 Median lethal concentration (LC₅₀) of different nematode strain against 3rd instar larvae Oryctes rhinoceros at 48, 72 and 96 h after treatment (DAT) (n = 12)

Incubation period (hrs.)	EPN species	LC₅₀ (IJs)	Fiducial limit (95%)	Slope ± SE	Pearson’s χ²	P value
48	Steinernema carpocapsae	1809^a	1262–2759	1.50 ± 0.21	34.19
	Steinernema abbasi	3001^a	2363–4058	1.42 ± 0.16	76.08	< 0.001
	Heterorhabditis indica	15,859^b	8496–55,501	1.10 ± 0.21	29.05
72	Steinernema carpocapsae	1078^a	897–1288	1.84 ± 0.16	32.83
	Steinernema abbasi	1663^b	1347–2083	1.48 ± 0.15	96.08	< 0.001
	Heterorhabditis indica	9780^c	5671–26,502	0.94 ± 0.16	34.20
96	Steinernema carpocapsae	854^a	703–1024	1.80 ± 0.16	29.85
	Steinernema abbasi	1489^b	1199–1866	1.43 ± 0.15	94.04	< 0.001
	Heterorhabditis indica	5353^c	3633–9858	1.04 ± 0.16	25.72

EPN: Entomopathogenic nematodes; Different letters indicate significant differences among LC₅₀ values within nematode species based on overlap of 95% Fiducial Limit