Antagonistic activities of endophytic bacteria isolated from rice roots against the fungus Magnaporthe oryzae, a causal of rice blast disease

Egyptian Journal of Biological Pest Control

Table 4 Control efficacy of culture broth of strains ND06 and ND10 against rice blast in greenhouse conditions

Treatments	Incidence rate (%)		Disease index		Control efficacy (%)
Treatments	ND06	ND10	ND06	ND10	ND06	ND10
T1	–	–	–	–	–	–
T2	92.3^a	95.1^a	65.2^a	66.1^a	–	–
T3	77.8^b	78.1^b	31.2^b	32.3^b	49.7^b	50.1^c
T4	39.8^c	40.7^c	14.8^c	15.1^c	73.2^a	73.9^b
T5	32.5^d	34.9^d	13.7^c	12.8^d	74.7^a	75.6^a

T1: A blank solution without fungus (M. oryzae) and endophytic bacteria; T2: A mixture of LB medium only and conidial suspension (5 × 10⁵ conidia/ml) of M. oryzae. added with 0.025% Tween 20; T3: A mixture of the fungicide tricyclazole (750 μg/mL) and conidial suspension added with 0.025% Tween 20; T4: A mixture of culture broth from 3-day-old cultures (10%, v/v) and conidial suspension amended with 0.025% Tween 20; T5: Rice seeds were germinated in the 3-day-old culture broth of selected strains for 24 h. After rising with autoclaved water, the geminated seeds were planted in a pot. The seedlings at the five-leaf stage were sprayed with conidial suspensions. Means ± standard errors within each column, followed by the same letter were non-significantly (P < 0.05) different according to the HSD test