Antagonistic activities of endophytic bacteria isolated from rice roots against the fungus Magnaporthe oryzae, a causal of rice blast disease

Egyptian Journal of Biological Pest Control

Table 1 Treatments applied in pot experiments

Treatments	Preparations
T1	A blank solution without fungus (M. oryzae) and endophytic bacteria
T2	A mixture of LB medium only and conidial suspension (5 × 10⁵ conidia/mL) of M. oryzae. added with 0.025% Tween 20
T3	A mixture of the fungicide tricyclazole (750 μg/mL) and conidial suspension was added with 0.025% Tween 20
T4	A mixture of culture broth from 3-day-old cultures (10%, v/v) and conidial suspension amended with 0.025% Tween 20
T5	Rice seeds were germinated in a 3-day-old culture broth of selected strains for 24 h. After rising with autoclaved water, the geminated seeds were planted in a pot. The seedlings at the five-leaf stage were sprayed with conidial suspensions

Each treatment included three pots (20 × 25 × 30 cm) with 30 seedlings per pot. The experiments were done in triplicates