Insecticidal effect of entomopathogenic nematodes and the cell-free supernatants from their symbiotic bacteria against different larval instars of Agrotis segetum (Denis & Schiffermüller) (Lepidoptera: Noctuidae)

Egyptian Journal of Biological Pest Control

Table 3 The efficacy of infective juveniles of different entomopathogenic nematodes against 3rd and 4th instar Agrotis segetum larvae at the 1st, 2nd, and 3rd day after treatment (DAT) under laboratory conditions (25 ± 1 °C, R.H. 60%)

Stage	Concentrations	Time (hrs)	EPNs*
Stage	Concentrations	Time (hrs)	ÜTP-5	MÇB-8	AVB-15
L3	10 IJs/cm²	24	2.5A^aa^bA^c	2.5AaA	2.5AaA
		48	30.0BaA	17.5BbA	20.0BbA
		72	62.5CaA	40.0CbA	45.0CbA
	50 IJs/cm²	24	7.5AaA	5.0AaA	5.0AaA
		48	37.5BaA	27.5BaA	32.5BaAB
		72	77.5CaB	52.5CbAB	52.5CbA
	100 IJs/cm²	24	12.5AaA	10.0AaA	10.0AaA
		48	50.0BaB	52.5BaB	52.5BaB
		72	82.5CaB	62.5CbB	60.0CbA
L4	10 IJs/cm²	24	5.0AaA	2.5AaA	2.5AaA
		48	25.0BaA	32.5BabA	45.0BbA
		72	62.5CaA	65.0CaA	67.5CaA
	50 IJs/cm²	24	12.5AaA	7.5AaA	10.0AaA
		48	37.5BaA	52.5BbB	57.5BbB
		72	75.0CaA	77.5CaA	82.5CaB
	100 IJs/cm²	24	15.0AaA	12.5AaA	15.0AaA
		48	55.0BaB	70.0BbC	62.5BabB
		72	95.0CaB	95.0CaB	97.5CaB

ÜTP-5 Steinernema feltiae ÜTP-5 strain; MÇB-8 Steinernema feltiae MÇB-8 strain; AVB-15 Heterorhabditis bacteriophora AVB-15 strain; L3 Third larval instar of Agrotis segetum; L4 Fourth larval instar of A. segetum
*EPNs Entomopathogenic nematodes
^aDifferent capital letters show statistically significant differences among exposure times for each concentration (Tukey, P ≤ 0.05)
^bDifferent lowercase letters show statistically significant differences among nematode strains for each exposure time (Tukey, P ≤ 0.05)
^cDifferent italic capital letters show statistically significant differences among concentrations for the same nematode strain and exposure time (Tukey, P ≤ 0.05)