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Table 1 Oligonucleotide primers and PCR conditions used for screening of cry and vip3 genes

From: Characterization of indigenous Bacillus thuringiensis isolate RM11 toxic to the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

S. No. Primer Sequence 5′ → 3′ Amplicon size (bp) Temperature profile References
1 cry1 FP: 5′-CATGATTCATGCGGCAGATAAAC-3′
RP: 5′-TTGTGACACTTCTGCTTCCCATT-3′
274–277 94 °C for 2 min
94 °C for 40 s
62 °C for 40 s
72 °C for 1 min
72 °C for 7 min
30 cycles
Ben-Dov et al. (1997)
2 cry1Ac FP: 5′-GTATGCTTCTGTAACCCCGATTCACCTC-3′
RP: 5′-CCTGCAGTCCCACTAAAATTTCTAACACCTACTA-3′
195 94 °C for 2 min
94 °C for 40 s
53.6 °C for 45 s
72 °C for 45 s
72 °C for 10 min
30 cycles
Alberola et al. (1999)
3 cry2 FP: 5′-GTTATTCTTAATGCAGATGAATGGG-3′
RP: 5′-CGGATAAAATAATCTGGGAAATAGT-3′
689–701 94 °C for 2 min
94 °C for 40 s
60 °C for 40 s
72 °C for 40 s
72 °C for 10 min
30 cycles
Ben-Dov et al. (1997)
4 vip3A FP: 5′-CCTCTATGTTGAGTGATGTA-3′
RP: 5′-CTATACTCCGCTTCACTTGA-3′
1000 94 °C for 5 min
94 °C for 1 min
55 °C for 1 min
72 °C for 40 s
72 °C for 10 min
35 cycles
Jain et al. (2017)