Table 1 Oligonucleotide primers and PCR conditions used for screening of cry and vip3 genes
S. No. | Primer | Sequence 5′ → 3′ | Amplicon size (bp) | Temperature profile | References |
|---|---|---|---|---|---|
1 | cry1 | FP: 5′-CATGATTCATGCGGCAGATAAAC-3′ RP: 5′-TTGTGACACTTCTGCTTCCCATT-3′ | 274–277 | 94 °C for 2 min 94 °C for 40 s 62 °C for 40 s 72 °C for 1 min 72 °C for 7 min 30 cycles | Ben-Dov et al. (1997) |
2 | cry1Ac | FP: 5′-GTATGCTTCTGTAACCCCGATTCACCTC-3′ RP: 5′-CCTGCAGTCCCACTAAAATTTCTAACACCTACTA-3′ | 195 | 94 °C for 2 min 94 °C for 40 s 53.6 °C for 45 s 72 °C for 45 s 72 °C for 10 min 30 cycles | Alberola et al. (1999) |
3 | cry2 | FP: 5′-GTTATTCTTAATGCAGATGAATGGG-3′ RP: 5′-CGGATAAAATAATCTGGGAAATAGT-3′ | 689–701 | 94 °C for 2 min 94 °C for 40 s 60 °C for 40 s 72 °C for 40 s 72 °C for 10 min 30 cycles | Ben-Dov et al. (1997) |
4 | vip3A | FP: 5′-CCTCTATGTTGAGTGATGTA-3′ RP: 5′-CTATACTCCGCTTCACTTGA-3′ | 1000 | 94 °C for 5 min 94 °C for 1 min 55 °C for 1 min 72 °C for 40 s 72 °C for 10 min 35 cycles | Jain et al. (2017) |