Compatibility of entomopathogenic nematodes with insecticides against the cabbage white butterfly, Pieris rapae L. (Lepidoptera: Pieridae)

Egyptian Journal of Biological Pest Control

Table 1 Primers of the two target genes and a reference gene of Pieris rapae used in this study

Target gene	Primers sequences	Reverse transcription	Primary denaturation	Amplification (40 cycles)			Dissociation curve (1 cycle)			References
Target gene	Primers sequences	Reverse transcription	Primary denaturation	Secondary denaturation	Annealing (Optics on)	Extension	Secondary denaturation	Annealing	Final denaturation	References
ß-actin	CGGTATGGGTCAGAAGGAC	50 °C 30 min	94 °C 15 min	94 °C 15 s	55 °C 30 s	72 °C 30 s	94 °C 1 min	55 °C 1 min	94 °C 1 min	Liu et al. (2018)
ß-actin	AGAAGGTGTGGTGCCAGAT	50 °C 30 min	94 °C 15 min	94 °C 15 s	55 °C 30 s	72 °C 30 s	94 °C 1 min	55 °C 1 min	94 °C 1 min
CYP6AE120	TGCTTGCGTGTGTGAAACTC	50 °C 30 min	94 °C 15 min	94 °C 15 s	60 °C 30 s	72 °C 30 s	94 °C 1 min	60 °C 1 min	94 °C 1 min
CYP6AE120	GAACACCCGAAGGGAGAGTG	50 °C 30 min	94 °C 15 min	94 °C 15 s	60 °C 30 s	72 °C 30 s	94 °C 1 min	60 °C 1 min	94 °C 1 min
PrGSTs1	CTTCCCTGTTAAGGCACTC	50 °C 30 min	94 °C 15 min	94 °C 15 s	60 °C 30 s	72 °C 30 s	94 °C 1 min	60 °C 1 min	94 °C 1 min	Liu et al. (2017)
PrGSTs1	TACACAGCGTCAACCACTT	50 °C 30 min	94 °C 15 min	94 °C 15 s	60 °C 30 s	72 °C 30 s	94 °C 1 min	60 °C 1 min	94 °C 1 min	Liu et al. (2017)