Table 3 Crystal protein profile and cry genes distribution in native Bt isolates
Parameters | Variations observed | No. of isolates (out of 59) |
|---|---|---|
Protein size | ⁓ 124 kDa | 20 |
⁓ 90 kDa | 8 | |
⁓ 70 kDa | 50 | |
⁓ 60 kDa | 2 | |
⁓ 55 kDa | 2 | |
⁓ 45 kDa | 11 | |
⁓ 26 kDa | 15 | |
Number of protein bands in SDS-PAGE | One | 22 |
Two | 25 | |
Three | 11 | |
Four | 1 | |
Number of PCR-positive isolates for cry genes | cry1 | 30 |
cry2 | 9 | |
cry9 | 0 | |
vip3 | 9 | |
cry1 + cry2 | 10 | |
cry1 + cry2 + vip3 | 5 | |
cry1 + vip3 | 9 | |
cry2 + vip3 | 5 |