Skip to main content

Table 2 Mortality percentage of Rhynchophorus ferrugineus populations treated with Bt-k (70 µg g−1), Beauveria bassiana (1 × 107 conidia ml−1) and Heterorhabditis bacteriophora (300 IJs ml−1) applied alone or in combination after 7 days of exposure

From: Biocontrol potential of entomopathogenic fungi, nematodes and bacteria against Rhynchophorus ferrugineus (Olivier)

Stage

Treatments

Motility%

Layyah

D.G. Khan

Muzaffargarh

R.Y. Khan

F

P

Larvae

Bt-k

9.47 ± 0.78e

6.47 ± 0.55d

6.08 ± 0.78d

7.93 ± 0.71e

0.38

0.77

Bb

13.48 ± 1.18de

10.47 ± 1.12 cd

8.39 ± 1.01 cd

11.68 ± 1.16de

0.30

0.82

EPN

25.57 ± 1.39 cd

21.57 ± 1.38bc

19.58 ± 1.17bc

23.45 ± 1.13 cd

0.62

0.60

Bt-k + Bb

32.28 ± 1.61bc

28.36 ± 1.46ab

25.06 ± 1.30b

30.17 ± 1.65bc

1.28

0.29

Bt-k + EPN

45.37 ± 2.15ab

33.73 ± 1.50ab

28.08 ± 1.57ab

42.11 ± 2.36ab

6.46

 ≤ 0.05

Bb + EPN

51.68 ± 2.34a

42.19 ± 2.51a

39.40 ± 2.14a

46.82 ± 2.54a

2.05

0.12

F

24.8

30.5

18.8

14.4

P

 ≤ 0.05

 ≤ 0.05

 ≤ 0.05

 ≤ 0.05

Adults

Bt-k

6.54 ± 0.70d

4.69 ± 0.49d

4.02 ± 0.53c

5.46 ± 0.74d

0.24

0.87

Bb

10.46 ± 1.12d

8.41 ± 0.87 cd

7.56 ± 1.04bc

9.29 ± 1.09 cd

0.16

0.92

EPN

19.18 ± 1.24 cd

14.90 ± 1.01bcd

13.22 ± 1.35bc

16.51 ± 1.21 cd

0.72

0.54

Bt-k + Bb

24.70 ± 1.65bc

21.24 ± 1.96abc

19.69 ± 1.56ab

22.06 ± 1.56bc

0.31

0.81

Bt-k + EPN

35.41 ± 2.35ab

28.84 ± 2.11ab

27.09 ± 1.75b

31.52 ± 2.13ab

0.90

0.45

Bb + EPN

39.73 ± 2.09a

32.37 ± 2.03a

30.89 ± 2.05a

36.08 ± 2.45a

1.38

0.26

F

19.2

8.90

12.4

14.4

 

P

 ≤ 0.05

 ≤ 0.05

 ≤ 0.05

 ≤ 0.05

  1. Mortality% followed by the same letter within each treatment and insect populations are not significantly different; HSD test P ≤ 0.05 (Bt-k: Bacillus thuringiensis var. kurstaki, Bb: Beauveria bassiana, EPN: Heterorhabditis bacteriophora)