Skip to main content

Table 1 List of primers used for amplification of RNA polymerase (RPB1) gene and non-coding region of internal transcribed spacer (ITS) sequence of the nuclear ribosomal DNA

From: Occurrence of Metarhizium spp. isolated from forest samples in South India and their potential in biological control of banana stem weevil Odoiporus longicollis Oliver

Gene Product length (partial) Annealing TM Sequence References
RPB-F1
RPB-R1
450–550 55.9 F-5′GARTGYCCDGGDCAYTTYGG-3′
R-5′CCNGCDATNTCRTTRTCCATRTA-3′
Stiller and Hall (1997)
RPB-F2
RPB-R2
450–500 51.3 F-5′CGRACMYTRCCYCATTTCACAA-3′
R-5′TTGAGCGGAAGYTGCATCATCTCC-3′
Bidochka et al. (2001)
RPB-F3
RPB-R3
450–600 47.1 F-5′CGRACMYTRCCYCATTTCACAA-3′
R-5′TTCARRAARGCCATSGCRCCWTC-3′
Bischoff et al. (2006, 2009)
ITS1
ITS2
600–700 57.0 F-5′CCTCCGCTTATTGATATGC-3′
R-5′GGAAGTAAAAGTCGTAACAAGG-3′
Curran et al. (1994), Entz et al. (2008)
NS1
AB28
650–750 53.0 F-5′CTCTCCAAACTCGGTCATTT-3′
R-5′ATATGCTTAAGTTCAGCGGGT-3′
Curran et al. (1994), Entz et al. (2008)