Table 2 Phytophthora genus and species specific primers sequence
Primer name | Sequence (5′-3′) | Direction | Fragment size (bp) | Specificity |
|---|---|---|---|---|
Ph2 | ATACTGTGGGGACGAAAGTC | Forward | 700 | At genus level |
ITS4 | TCCTCCGCTTATTGATATGC | Reverse | ||
Pn5B | GAACAATGCAACTTATTGGACGTTT | Forward | 120 | At species level |
Pn6 | AACCGAAGCTGCCACCCTAC | Reverse |