Table 1 Characteristics of primers for screening of cry-type and 16S-ITS rDNA region genes
Primer name | Melting temperature (°C) | Sequence (5' → 3') | Reference |
|---|---|---|---|
16S ITS rDNA(F) | 60 | AGAGTTTGATCCTGGCTCAG | Yılmaz et al., (2012) |
16S ITS rDNA(R) | 53 | CAAGGCATCCACCGT | Â |
Spcry1Aa (F) | 54 | TTCCCTTTATTTGGGAATGC | Seifinejad et al. (2008) |
UNcry1 (R) | 51 | MDATYTCTAKRTCTTGACTA | Â |
Lep2 (F) | 58 | CCGAGAAAGTCAAACATGCG | Boukedi et al. (2016) |
Lep2 (R) | 58 | TACATGCCCTTTCACGTTCC | Â |
St2C (F) | 56 | GGGACATTCCTTCGTTTCG | BenFarhat-Touzri et al. (2016) |
St2Cinv(R) | 54 | GGTGTTCCAGATCTTTGAAC | Â |
St1D (F) | 49 | ATGGAAATAAATAATCAAAACC | BenFarhat-Touzri et al. (2016) |
St1Dinv (R) | 51 | ACTAGATTGGATACCTGATC | Â |
UNcry2 (F) | 55 | CGGATAAAATAATCTGGGAAATAGT | Salehi Jouzani et al. (2008) |
SPcry2Aa (R) | 57 | GAGATTAGTCGCCCCTATGAG | Â |
UNcry2 (F) | 55 | CGGATAAAATAATCTGGGAAATAGT | Salehi Jouzani et al. (2008) |
SPcry2Ab (R) | 65 | TGGCGTTAACAATGGGGGGAGAAAT | Â |