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Table 1 PCR primers used in the species-specific multiplex PCR. Primers were designed in the Primer3 (v.0.4.0) software (Rozen and Skaletsky, 2000) from complete sequences of ITS2 rRNA

From: Rapid molecular identification of Trichogramma (Hymenoptera: Trichogrammatidae) parasitizing the eggs of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae) in Serbia

Species Primer name Primer sequence Amplicon size (bp)
All ITS2for 5′-TGTGAACTGCAGGACACATG-3′ -
T. brassicae T.bras-R 5′-CGACGTATCACACTGCTCCT-3′ 199
T. evanescens T.evan-R 5′-GCCGTCGTTACCTACTTTCG-3′ 480