Table 1 PCR primers used in the species-specific multiplex PCR. Primers were designed in the Primer3 (v.0.4.0) software (Rozen and Skaletsky, 2000) from complete sequences of ITS2 rRNA
Species | Primer name | Primer sequence | Amplicon size (bp) |
|---|---|---|---|
All | ITS2for | 5′-TGTGAACTGCAGGACACATG-3′ | - |
T. brassicae | T.bras-R | 5′-CGACGTATCACACTGCTCCT-3′ | 199 |
T. evanescens | T.evan-R | 5′-GCCGTCGTTACCTACTTTCG-3′ | 480 |