Pathogenicity of Metarhizium rileyi (Farlow) Kepler, S.A. Rehner and Humber isolates against Spodoptera litura (Fabricius) and their extracellular enzymatic activities

Egyptian Journal of Biological Pest Control

Table 3 Protease activity of Metarhizium rileyi isolates on different days after inoculation

Treatments	Protease activity on alternate days (U/ml)* (mean ± S.E.)**
Treatments	2nd day	4th day	6th day	8th day	10th day	Mean
*M. rileyi* NIPHM	0.72 ± 0.03^Ad	0.98 ± 0.03^Ac	1.67 ± 0.01^Ab	2.30 ± 0.07^Aa	2.20 ± 0.02^Aa	1.58
*M. rileyi* MTCC 4254	0.69 ± 0.02^Ad	0.94 ± 0.02^Ad	1.71 ± 0.03^Ac	2.39 ± 0.10^Aa	2.11 ± 0.02^BAb	1.57
*M. rileyi* MTCC 10395	0.53 ± 0.01^Bd	0.84 ± 0.03^Ac	1.20 ± 0.07^Bb	2.14 ± 0.06^Aa	2.01 ± 0.03^Ba	1.34
Mean	0.65	0.92	1.53	2.28	2.10	–

Mean values followed by the same letter (a, b, c) in the horizontal column are not significantly different at 0.05% level of probability using Tukey’s post hoc test. Mean values followed by the same letter (A, B, C) in the vertical column are not significantly different at 0.05% level of probability using Tukey’s post hoc test
**Values are mean ± standard error of two replicates
*1 U/ml corresponds to 1 μg of tyrosine produced min⁻¹ ml⁻¹