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Table 1 Primers, amplicon sizes, and references used in the study

From: Evaluation of bacteriophages in the biocontrol of Pseudomonas syringae pv. syringae isolated from cankers on sweet cherry (Prunus avium L.) in Turkey

Target gene Primer names Oligonucleotide sequence Cycle conditions Amp. size References
irp1 PSYE2 5′-GGCACCTGGAACAGG-3′ The program was as follows: an initial denaturation at 94 °C for 5 min; 37 cycles at 94 °C for 1 min, 64 °C for 1 min, and 72 °C for 1 min; and a final elongation at 72 °C for 6 min 943 bp Bultreys et al. (2006)
PSYE2R 5′-GCCAGATCGTCCATCAT-3′
syrB Primer B1 5′-CTTTCCGTGGTCTTGATGAGC-3′ The program was as follows: an initial denaturation at 94 °C for 5 min; 35 cycles at 94 °C for 1.5 min, 60 °C for 1.5 min, and 72 °C for 3 min; and a final elongation at 72 °C for 10 min 752 bp Sorensen et al. (1998)
Primer B2 5′-TCGATTTTGCCGTGATGAGTC-3′
cfl Primer1 5′-GGCGCTCCCTCGCACTT-3′ The program was as follows: an initial denaturation at 93 °C for 2 min; 37 cycles at 93 °C for 1 min, 67 °C for 1 min, and 72 °C for 2 min; and a final elongation at 72 °C for 10 min 650 bp Bereswill et al. (1994)
Primer2 5′-GGTATTGGCGGGGGTGC-3′