Evaluation of bacteriophages in the biocontrol of Pseudomonas syringae pv. syringae isolated from cankers on sweet cherry (Prunus avium L.) in Turkey

Egyptian Journal of Biological Pest Control

Table 1 Primers, amplicon sizes, and references used in the study

Target gene	Primer names	Oligonucleotide sequence	Cycle conditions	Amp. size	References
irp1	PSYE2	5′-GGCACCTGGAACAGG-3′	The program was as follows: an initial denaturation at 94 °C for 5 min; 37 cycles at 94 °C for 1 min, 64 °C for 1 min, and 72 °C for 1 min; and a final elongation at 72 °C for 6 min	943 bp	Bultreys et al. (2006)
irp1	PSYE2R	5′-GCCAGATCGTCCATCAT-3′		943 bp	Bultreys et al. (2006)
syrB	Primer B1	5′-CTTTCCGTGGTCTTGATGAGC-3′	The program was as follows: an initial denaturation at 94 °C for 5 min; 35 cycles at 94 °C for 1.5 min, 60 °C for 1.5 min, and 72 °C for 3 min; and a final elongation at 72 °C for 10 min	752 bp	Sorensen et al. (1998)
syrB	Primer B2	5′-TCGATTTTGCCGTGATGAGTC-3′		752 bp	Sorensen et al. (1998)
cfl	Primer1	5′-GGCGCTCCCTCGCACTT-3′	The program was as follows: an initial denaturation at 93 °C for 2 min; 37 cycles at 93 °C for 1 min, 67 °C for 1 min, and 72 °C for 2 min; and a final elongation at 72 °C for 10 min	650 bp	Bereswill et al. (1994)
cfl	Primer2	5′-GGTATTGGCGGGGGTGC-3′		650 bp	Bereswill et al. (1994)