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Table 1 Details of the baculovirus target core genes, primer sequences, and annealing temperature for amplification of DNA fragments

From: Characterization of alphabaculovirus: HearNPV-IIPR05 isolate infecting Helicoverpa armigera (Hubner) larvae

Target genes Primer sequence Amplicon size (bp) Annealing temperature (°C)
Polyhedrin (polh) F: 5′-ACTCGTTACAGTTACAGCCCTACT-3′ 659 54
R: 5′-CAGTGTATAGCGGAGCGTCA-3′
Per os infectivity factor-1 (pif-1) F: 5′-CCGCTACCAATTCACGAAG-3′ 1392 48
R: 5′-TCATGTGTACGTGGAATAATTGG-3′
Late expression factor-8 (lef-8) F: 5′-GACGTGATTGCCGATTTC-3′ 940 58
R: 5′-TTTCAATGTTGTTGTTGCTG-3′
F: 5′-GATCGTAGACACTATAGTCAATGG-′3′ 855 60
R: 5′-GCGTTTTTCAAATTGGTC-3′
F: 5′-CAATAATCGAAAAGGACACAG-3′ 949 58
R: 5′-TTGAAAATATTATCGGGAGTG-3′