Fig. 5

PCR amplification of polh, lef-8, and pif-1 gene coding regions. a Amplification of polh gene. M—1 kb ladder; 1 and 2—659 bp (2 replicas). b Amplifications of lef-8 gene. M—50 bp step up ladder; 1—940 bp; 2—855 bp; and 3—949 bp. c Amplification of pif-1 gene. M—50 bp step up ladder; 1 and 2—1392 bp (2 replicas)