Fig. 5

The SDS-PAGE analysis of δ-endotoxin (130 kD) for Bacillus thuringiensis culture obtained from different modes of cultivation. Cultures, collected at 48 h of cultivation, were applied to 10% polyacrylamide gel and stained with Coomassie brilliant blue. M, standard marker; 1, intermittent fed-batch cultivation with feeding at exponential growth phase and stationary growth phase; 2, fed-batch cultivation with feeding at stationary growth phase; 3, fed-batch cultivation with feeding at lag growth phase; 4, fed-batch cultivation with feeding at exponential growth phase; and 5, batch cultivation