Table 1 Characteristics of universal primers for cry1, cry2, cry3, cry4, and cry7, 8 group genes
| Primer pair | Sequence of primer | Gene recognized | Expected size (bp) |
|---|---|---|---|
|
Un1(d) Un1(r) |
5′CATGATTCATGCGGCAGATAAAC-3′ 5′-TTGTGACACTTCTGCTTCCCATT-3′ | cry1 |
274 277 |
|
Un2(d) Un2(r) |
5′-GTTATTCTTAATGCAGATGAATGGG-3′ 5′-CGGATAAAATAATCTGGGAAATAGT-3′ | cry2 |
689 701 |
|
Un3(d) Un3(r), |
5′CGTTATCGCAGAGAGATGACATTAAC3′ 5′-CATCTGTTGTTTCTGGAGGCAAT-3′ | cry3 |
589 595 604 |
|
Un4(d) Un4(r) |
5′-GCATATGATGTAGCGAAACAAGCC3′ 5′-GCGTGACATACCCATTTCCAGGTCC-3′ | cry4 | 439 |
|
Un7,8(d) Un7,8(r) |
5′AAGCAGTGAATGCCTTGTTTAC-3′ 5′-CTTCTAAACCTTGACTACTT-3′ | cry7 and cry8 |
420 423 |