Table 2 Influence of successive subculture on the activity of Pr1 enzyme and the LT₅₀ values of ARSEF4556, AUMC 3262, and V275 after 15 days growth on SDAY at 25 ± 2 °C

1st

0.73 ± 0.015^a

0.68 ± 0.056^a

0.77 ± 0.025^a

2.27 ± 0.07^b

2.14 ± 0.04^b

1.69 ± 0.12^c

3rd

0.72 ± 0.013^a

0.73 ± 0.030^a

0.77 ± 0.016^a

2.34 ± 0.09^b

2.07 ± 0.02^bc

2.41 ± 0.02^a

5th

0.72 ± 0.014^a

0.71 ± 0.11^a

0.73 ± 0.036^a

2.21 ± 0.05^b

1.92 ± 0.03^c

2.05 ± 0.03^b

7th

0.73 ± 0.002^a

0.66 ± 0.10^a

0.79 ± 0.010^a

2.71 ± 0.01^a

2.21 ± 0.05^ab

2.05 ± 0.03^b

9th

0.73 ± 0.014^a

0.70 ± 0.021^a

0.74 ± 0.027^a

2.36 ± 0.02^b

2.34 ± 0.09^a

2.27 ± 0.07^ab