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Table 2 Influence of successive subculture on the activity of Pr1 enzyme and the LT50 values of ARSEF4556, AUMC 3262, and V275 after 15 days growth on SDAY at 25 ± 2 °C

From: Comparative study of fungal stability between Metarhizium strains after successive subculture

Subcultures Pr1 (μmol ml−1 min−1 ) LT50
ARSEF4556 AUMC 2362 V275 ARSEF 4556 AUMC3262 V275
1st 0.73 ± 0.015a 0.68 ± 0.056a 0.77 ± 0.025a 2.27 ± 0.07b 2.14 ± 0.04b 1.69 ± 0.12c
3rd 0.72 ± 0.013a 0.73 ± 0.030a 0.77 ± 0.016a 2.34 ± 0.09b 2.07 ± 0.02bc 2.41 ± 0.02a
5th 0.72 ± 0.014a 0.71 ± 0.11a 0.73 ± 0.036a 2.21 ± 0.05b 1.92 ± 0.03c 2.05 ± 0.03b
7th 0.73 ± 0.002a 0.66 ± 0.10a 0.79 ± 0.010a 2.71 ± 0.01a 2.21 ± 0.05ab 2.05 ± 0.03b
9th 0.73 ± 0.014a 0.70 ± 0.021a 0.74 ± 0.027a 2.36 ± 0.02b 2.34 ± 0.09a 2.27 ± 0.07ab
  1. Each value represents the mean of three replicates. The means within a column followed by the same letter are not significantly different (LSD test, P > 0.05). LT50 median lethal time. Pr1 activity is expressed as μmol ml1 min1 released from succinyl-ala-ala-pro-phe-p-nitroanilide