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Fig. 2 | Egyptian Journal of Biological Pest Control

Fig. 2

From: Ingestion of bacteria expressing dsRNA to maggots produces severe mortality and deformities in fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Fig. 2

cDNA-PCR amplification of ECR and RPL19 genes fragments, their endogenous expression in adult (A) and maggot (M) Bactrocera dorsalis, and dsRNA expression construct preparation and dsRNA production in bacteria. a, b cDNA-PCR amplification of ecr and rpl19 genes-specific sequences from adult and maggot stages of fruit fly with gene-specific primers. Where L: 1 kb DNA ladder, M: maggot, A: adult. Lower panel gel picture of b shows the expression of actin gene as an internal control. The relative transcript level of each of the genes in whole body of fruit fly is represented graphically in c. Mean with SEM of three independent experiments were shown. Asterisks and ns indicate significant and non-significant differences (**p < 0.01 and ns, non-significant, Independent t test). d Diagrammatically represented strategy of cloning ECR and RPL19 gene fragments in pL4440 vector at multiple cloning sites (MCS) in between two T7 promoters and double-stranded (ds) RNA production upon IPTG induction in HT115 bacterial strain. e Total RNA was extracted from bacteria. The position of dsRNA produced is indicated with arrowhead

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