Table 1 List of oligonucleotide primers used for the characterization of Sclerotium rolfsii at molecular level
From: Use of Neem leaves as soil amendment for the control of collar rot disease of chickpea
No. | Primer name | 5′ to 3′ sequence | Annealing temperature |
|---|---|---|---|
1 | ITS 1 forward | TCCGTAGGTGAACCTGCGG | 60 °C |
2 | ITS 4 reverse | TCCTCCGCTTATTGATATGC | |
3 | β-tubulin forward | GGTAACCAAATCGGTGCTGCTTTC | 62 °C |
4 | β-tubulin reverse | ACCCTCAGTGTAGTGACCCTTGGC |