Morphological, molecular, and pathogenicity characteristics of the native isolates of Metarhizium anisopliae against the tomato leafminer, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in Ethiopia

Ayele, Birhan Aynalem; Muleta, Diriba; Venegas, Juan; Assefa, Fassil

doi:10.1186/s41938-020-00261-w

Egyptian Journal of Biological Pest Control

Table 3 Summary of Probit analysis, calculated lethal time, and concentration-mortality response at 10 days of post inoculation to third larval instar of Tuta absoluta

From: Morphological, molecular, and pathogenicity characteristics of the native isolates of Metarhizium anisopliae against the tomato leafminer, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in Ethiopia

Spore ml⁻¹	AAUM43	AAUM76	AAUM20	AAUM80	AAUM78	AAUM39
Mortality (mean ± SE)
10⁴	50.33 ± 4.41^e	52.33 ± 4.41^e	50.00 ± 5.77^e	55.00 ± 2.89^e	78.33 ± 4.41^a	65.00 ± 10.41^c
10⁵	61.67 ± 10.1^c	61.67 ± 6.01^c	61.67 ± 4.41^c	60.00 ± 5.77^c	83.33 ± 1.67^ab	73.33 ± 4.41^bc
10⁶	81.67 ± 3.33^b	70.00 ± 5.77^d	83.33 ± 6.01^bc	76.67 ± 4.41^c	86.67 ± 1.67^b	83.33 ± 1.67^bc
10⁷	95.00 ± 1.89^a	80.00 ± 5.00^bc	89.00 ± 2.89^ab	88.33 ± 1.67^b	93.33 ± 1.67^a	91.33 ± 1.67^b
10⁸	95.00 ± 0.00^a	86.67 ± 1.67^c	91.67 ± 1.67^b	90.00 ± 2.89^b	96.67 ± 1.67^a	93.33 ± 1.67^ab
LT₅₀ (mean ± SE)
10⁴	5.33 ± 0.58^c	4.00 ± 1.00^b	4.00 ± 1.00^b	4.00 ± 1.00^b	3.93 ± 0.90^ab	3.50 ± 0.50^a
10⁶	3.00 ± 0.00^b	3.60 ± 0.53^a	3.33 ± 0.58^b	3.87 ± 0.12^ab	3.33 ± 0.58^b	3.00 ± 0.00^b
10⁸	2.81 ± 0.00^b	2.98 ± 0.00^a	2.87 ± 0.12^b	2.93 ± 0.12^a	1.93 ± 0.00^a	2.80 ± 0.20^b
Summary of Probit analysis
LC₅₀	1.4 × 10⁴	7 × 10⁴	4.3 × 10⁴	4.1 × 10⁴	1.2 × 10³	2.9 × 10³
95% FL	4 × 10³–7.2 × 10⁴	2.8 × 10³–3.6 × 10⁵	3.8 × 10³–1.7 × 10⁵	2.5 × 10³–1.8 × 10⁵	8.3 × 10²–3.8 × 10³	1.9 × 10²–2.9 × 10⁴
LC₉₀	8.2 × 10⁶	1.7 × 10⁸	1.6 × 10⁷	2.8 × 10⁷	3.5 × 10⁶	7.1 × 10⁶
95% FL	1.6 × 10⁶–3.6 × 10⁷	1.6 × 10⁷–1.1 × 10⁹	3.1 × 10⁶–4.5 × 10⁸	4.6 × 10⁶–1.7 × 10⁸	1.2 × 10⁴–3.4 × 10⁶	1 × 10⁶–1.7 × 10⁷
Int ± SE	1.93 ± 0.66	1.83 ± 0.66	2.32 ± 0.64	2.10 ± 0.63	0.59 ± 0.80	1.31 ± 0.66
S ± SE	4.6 ± 0.12	3.8 ± 0.10	5 ± 0.11	4.5 ± 0.11	3.4 ± 0.15	3.8 ± 0.12
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05

Letters in the columns (a, b, c, d, and e,) are ranks of the mean
FL fiducial limit, SE standard error, LT lethal time taken to kill 50% of experimental organisms, LC lethal concentration, Int intercept, S slope

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