Table 5 Bands generated and polymorphism percentages obtained from RAPD-PCR primers of T. tabaci after exposure to high concentrations of plant extracts and Aerosol 200® at 4 ml/l
Primer | No. bands generated | Polymorphic bands compared to control | Average of Polymorphic bands | Polymorphism % | Average of polymorphism within primer | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
C | M | F | Ca | Ch | N | M | F | Ca | Ch | N | M | F | Ca | Ch | N | |||
OPA/08 | 6 | 5 | 6 | 6 | 4 | 6 | 4 | 1 | 5 | 1 | 5 | 3.2 | 80 | 16 | 83 | 25 | 83 | 57.4% |
OPA/11 | 12 | 10 | 9 | 7 | 12 | 12 | 4 | 5 | 7 | 2 | 8 | 5.2 | 40 | 55 | 100 | 16 | 66 | 55.4% |
OPA/16 | 8 | 7 | 7 | 10 | 7 | 8 | 6 | 1 | 8 | 1 | 7 | 4.6 | 85 | 14 | 80 | 10 | 87 | 55.2% |
OPA/19 | 9 | 8 | 9 | 5 | 9 | 9 | 6 | 0 | 5 | 0 | 6 | 3.4 | 75 | 0 | 100 | 0 | 66 | 48.2% |
OPAI/21 | 10 | 5 | 8 | 8 | 9 | 5 | 5 | 3 | 5 | 2 | 3 | 3.6 | 100 | 37 | 62 | 22 | 60 | 56.2% |
OPAL/20 | 11 | 10 | 11 | 9 | 11 | 11 | 7 | 0 | 6 | 4 | 7 | 4.8 | 70 | 0 | 66 | 36 | 63 | 47.0% |
OPAB/09 | 8 | 8 | 8 | 7 | 8 | 8 | 5 | 1 | 5 | 0 | 4 | 3.0 | 62 | 12 | 71 | 0 | 50 | 39.0% |
OPH/11 | 14 | 13 | 14 | 14 | 12 | 13 | 8 | 3 | 10 | 3 | 8 | 6.4 | 61 | 21 | 71 | 25 | 61 | 47.8% |
Average of polymorphism % | 71 | 17 | 73 | 16 | 67 | |||||||||||||