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Table 1 Primers employed for identification of Eubacterial antagonistic strains and screening for the antibiotic producing ability of fluorescent pseudomonades (Raaijmakers et al. 1997)

From: Microbial biodiversity as related to crop succession and potato intercropping for management of brown rot disease

Target gene

Primer

Sequence (5´-3´)

Amplicon size

16S rDNA

U968-f (eubacteria)

5′ACCGCGAAGAACCTTAC3′

450 bp

L1401-r (eubacteria)

5′-CGG TGT GTA CAA GACCC-3′

phlD1

Phl2a

GAGGACGTCGAAGACCACCA

745 bp

Phl2b

ACCGCAGCATCGTGTATGAG

phzCD2

PCA2a

TTGCCAAGCCTCGCTCCAAC

1150 bp

PCA3b

CCGCGTTGTTCCTCGTTCAT

  1. 1Gene responsible for producing 2,4-diacetylphloroglucinol (Phl) antibiotic
  2. 2Gene responsible for producing phenazine-1-carboxylic acid (PCA) antibiotic